Acidovorax facilis

(aka Pseudomonas facilis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Acidovorax facilis, (aka Pseudomonas facilis), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 64–65%. Acidovorax facilis is probably a rare gut coloniser. (Willems1990; Willems2005Bergey)



  • This organism has been recovered from human faeces, and lawn soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Willems1990); (Willems2005Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin;
  • 🧂
  • Salt tolerance:
  • tolerates 0.5% salt; doesn't tolerate 3.5% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; strain-variable at 37(d); doesn't grow at 41(d), 42℃;
  • ⥅
  • Substrates assimilated or utilised:
  • arabinose; L-arabinose; d_arabitol; fructose; galactose; glucose; glycerol; mannitol; mannose; ribose; sorbitol; alanine; β-alanine; γ-aminobutyrate; aspartate; glutamate; histidine; isoleucine; leucine; phenylalanine; proline; serine; threonine; tryptophan; tyrosine; valine; azelate; butyrate; fumarate; glycerate; 3-hydroxybutyrate; isovalerate; DL-lactate; malate; L-malate; propionate; pyruvate; sebacate; suberate; succinate; glutamine;
  • ±
  • Strain-dependent substrate utilisation:
  • D-alanine; methionine; norleucine; ornithine; acetate; glutarate; 4-hydroxybenzoate; lactate; levulinate; pimelate;
  • Active enzymes:
  • alkaline phosphatase; catalase; esterase C4; esterase lipase C8; Leu arylamidase; oxidase;
  • ±
  • Strain-dependent active enzymes:
  • urease;
    • SPECIAL FEATURES (Willems1990); (Willems2005Bergey);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • ONPG test:
  • not active
  • ✗
  • Haemolysis:
  • absent
  • ✓
  • Nitrate:
  • reduced
  • ✗
  • Nitrite:
  • not reduced
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant

    Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Comamonadaceae Genus:  Acidovorax Alt. name:  Pseudomonas facilis Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, and lawn soil
    DNA G+C(%):  64–65
    Lower T(℃):  30(+)
    Mid T(℃):  37(d)
    High T(℃):  41(d), 42(neg)
    NaCl 0.5-2%:  0.5(+)
    NaCl 3-5%:  3.5(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  + DNA:  neg Tween:  80(vr)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  neg Glucose:  neg Maltose:  neg Adonitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  + Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + D-Lyxose:  neg Mannose:  + Rhamnose:  neg Ribose:  + D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg Adonitol:  neg Amygdalin:  neg D-Arabitol:  + L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  neg Aesculin:  neg Ethanolamine:  neg Glucosamine:  neg Glycerol:  + Glycogen:  neg Inulin:  neg Mannitol:  + Me-α-D-Glc:  neg Salicin:  neg Sorbitol:  + Starch:  neg Xylitol:  neg Ala:  + D-Ala:  d β-Ala:  + 4-Aminobutyrate:  + 5-Aminovalerate:  neg Asp:  + Betaine:  neg L-Citrulline:  neg Cys:  neg Glu:  + Gly:  neg Histamine:  neg His:  + Ile:  + DL-Kynurenine:  neg Leu:  + Lys:  neg Met:  d Norleucine:  d Orn:  d Phe:  + Pro:  + Sarcosine:  neg Ser:  + Thr:  + Trigonelline:  neg Tryptamine:  neg Trp:  + Tyr:  + Val:  + Acetate:  d Aconitate:  neg Adipate:  neg Azelate:  + Benzoate:  neg Butyrate:  + Caprate:  neg Caproate:  neg Caprylate:  neg Citraconate:  neg Citrate:  neg Fumarate:  + Glutarate:  d Glycerate:  + Glycolate:  neg Heptanoate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  d Isobutyrate:  neg Isovalerate:  + Itaconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Lactate:  d DL-Lactate:  + Levulinate:  d Malate:  + L-Malate:  + Maleate:  neg Malonate:  neg L-Mandelate:  neg Mesaconate:  neg Nonanoate:  neg Phenylacetate:  neg Pimelate:  d Propionate:  + Pyruvate:  + Sebacate:  + Suberate:  + Succinate:  + D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  d Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg LeuAA:  + AlkalineP:  + AcidP:  vr Esterase(C4):  + EstLip(C8):  + Lipase(C14):  vr
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    penicillin:  Var(MIC50): 10; disc), MIC90: Var(10; disc
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)

    References

    SPECIFIC REFERENCES FOR ACIDOVORAX FACILIS
  • Willems1990 - Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov.
  • Willems2005Bergey - Bergey's manual of systematic bacteriology. Vol. 2, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria Part C. Family Comamonadaceae, Genus II. Acidovorax
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ACIDOVORAX FACILIS
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
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    • Added: April 08, 2021