Afipia broomeae

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Afipia broomeae is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 61.5%. Afipia broomeae is probably a rare gut coloniser. (Brenner1991)



  • This organism has been recovered from human faeces and clinical sources (sputum - CCUG). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. Could be a possible pathogen in humans, but unknown at this stage. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Brenner1991);
    Character Response
  • Substrates hydrolysed or digested:
  • urea;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 6% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows weakly at 35℃;
  • Active enzymes:
  • catalase; oxidase; urease;
    • SPECIAL FEATURES (Brenner1991);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S; indole;
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Brenner1991);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin-sulbactam; imipenem;
  • piperacillin;
  • Cephalosporins:
  • cefoxitin; cephalothin;
  • cefoperazone;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • ciprofloxacin;
  • Aminoglycosides:
  • tobramycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Bradyrhizobiaceae Genus:  Afipia Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and clinical sources (sputum - CCUG)
    DNA G+C(%):  61.5
    Lower T(℃):  25(+)
    Mid T(℃):  35(w)
    NaCl >6%:  6(neg)
    		Aesculin:  neg Urea:  + Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg Xylose:  w Lactose:  neg Maltose:  neg Sucrose:  neg Mannitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Citrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(2/4)
    ampicillin:  Var(MIC50): 8), MIC90: Var(8
    amp-sulb:  S(2/4)
    piperacillin:  R(64)
    ticarcillin:  Var(MIC50): 16), MIC90: Var(16
    imipenem:  S(<2)
    cefoperazone:  R(64)
    cefoxitin:  S(<4)
    cephalothin:  S(<4)
    amikacin:  Var(MIC50): 16), MIC90: Var(16
    gentamicin:  Var(MIC50): 8), MIC90: Var(8
    tobramycin:  S(4)
    ciprofloxacin:  S(4)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  R(>16)

    References

    SPECIFIC REFERENCES FOR AFIPIA BROOMEAE
  • Brenner1991 - Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR AFIPIA BROOMEAE
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
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    • Added: December 15, 2021