Alloprevotella rava

(aka Prevotella sp. oral taxon 302)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Alloprevotella rava, (aka Prevotella sp. oral taxon 302), is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 47%. Alloprevotella rava is probably a rare gut coloniser. (Downes2013)



  • This organism has been recovered from oral cavity. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • Probably an oral commensal that passes through the GI tract.
    • GENERAL CHARACTERISTICS (Downes2013);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin;
  • 💧
  • Bile tolerance:
  • Doesn't tolerate 20% bile
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5; doesn't tolerate pH 8; Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 42℃; Grows optimally at 35℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; lactose; maltose;
  • Active enzymes:
  • Ala arylamidase; alkaline phosphatase; N-Ac β-glucosaminidase; fucosidase; α-galactosidase; β-galactosidase; α-glucosidase; Leu-Gly arylamidase;
  • ±
  • Strain-dependent active enzymes:
  • Glu-Glu arylamidase;
    • SPECIAL FEATURES (Downes2013);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; succinate (major); isovalerate (trace);
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Downes2013); (Goldstein2013a);
    Class Active Resistant
  • Macrolides:
  • fidaxomicin;
  • Aminoglycosides:
  • kanamycin;
  • Heterocycles:
  • metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • colistin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Bacteroidia Order:  Bacteroidales Family:  Prevotellaceae Genus:  Alloprevotella Alt. name:  Prevotella sp. oral taxon 302 Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  oral cavity
    DNA G+C(%):  47
    Opt. T:  35℃
    Lower T(℃):  30(+)
    High T(℃):  42(+)
    Opt. pH:  7.0
    pH 4.2-5.9:  5(neg)
    pH 6.0-8.0:  8(neg)
    Bile reaction(%):  20(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  + Arginine:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Glucose:  + Mannose:  + Rhamnose:  neg Xylose:  neg Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Mannitol:  neg Sorbitol:  neg Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Ac-β-glcamnd:  + α-Fucosidase:  + α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + AlanineAA:  + GluGluAA:  d LeuGlyAA:  + AlkalineP:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Succinate:  Major(+) Isovalerate:  trace(+) H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    kanamycin:  R(1000; disc)
    fidaxomicin:  R(MIC50): >512, MIC90: >512, RNG: (64->512)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    vancomycin:  R(MIC50): 128, MIC90: >512, RNG: (64->512)
    metronidazole:  S(MIC50): 0.5, MIC90: 1, RNG: (0.25–1)
    colistin:  R(10; disc)

    References

    SPECIFIC REFERENCES FOR ALLOPREVOTELLA RAVA
  • Downes2013 - Description of Alloprevotella rava gen. nov., sp. nov., isolated from the human oral cavity, and reclassification of Prevotella tannerae Moore et al. 1994 as Alloprevotella tannerae gen. nov., comb. nov.
  • Goldstein2013a - Comparative in vitro activities of SMT19969, a new antimicrobial agent, against Clostridium difficile and 350 gram-positive and gram-negative aerobic and anaerobic intestinal flora isolates.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ALLOPREVOTELLA RAVA
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • De2020 - Metagenomic analysis of gut microbiome and resistome of diarrheal fecal samples from Kolkata, India, reveals the core and variable microbiota including signatures of microbial dark matter.
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    • Added: January 19, 2022