Amedibacillus dolichus

(aka Eubacterium dolichum)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Amedibacillus dolichus, (aka Eubacterium dolichum), is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 9 gut microbiome compilation studies or metastudies. The DNA G+C content is 38.1%. Amedibacillus dolichus is probably a common, although minor, coloniser of the gut. (Ikeyama2020; Moore1976; Paek2017; Wade2011Bergeys)



  • This organism has been recovered from human faeces (Ikeyama2020). The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Ikeyama2020); (Moore1976); (Paek2017); (Wade2011Bergeys);
    Character Response
  • 🧂
  • Salt tolerance:
  • Grows optimally with 1% salt.
  • 💧
  • Bile tolerance:
  • Resistant to 20% bile
  • 🌡
  • Temperature tolerance:
  • Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • xylose;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; arginine dihydrolase; N-Ac β-glucosaminidase; glutamic acid decarboxylase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Ikeyama2020); (Moore1976); (Paek2017); (Wade2011Bergeys);
    Character Response
  • ✓
  • Metabolites produced:
  • formate (minor); acetate (minor); butyrate (minor); lactate; H₂S;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Macrolides:
  • erythromycin;
  • Quinolines:
  • ciprofloxacin;
  • Polypep/ketides:
  • bacitracin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Erysipelotrichia Order:  Erysipelotrichales Family:  Erysipelotrichales Genus:  Amedibacillus Alt. name:  Eubacterium dolichum Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod Pigment:  neg
    Health:  Unknown
    Source:  human faeces (Ikeyama2020)
    DNA G+C(%):  38.1
    Opt. T:  37℃
    Bile reaction(%):  20(+)
    		Aesculin:  neg Urea:  neg Gelatin:  neg Starch:  neg Milk:  neg Meat:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Fructose:  neg Glucose:  neg Mannose:  neg Rhamnose:  neg Ribose:  neg Xylose:  + Cellubiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Amygdalin:  neg Aesculin:  neg Glycogen:  neg Starch:  neg Mannitol:  neg Sorbitol:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Urease:  neg Ac-β-glcamnd:  + α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  + GluDC:  + AlanineAA:  neg GluGluAA:  neg GlyAA:  neg LeuAA:  neg LeuGlyAA:  neg PyrrolidAA:  neg AlkalineP:  + AcidP:  + Esterase:  w EstLip(C8):  w
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  minor(+) Acetate:  minor(+) Butyrate:  minor(+) Lactate:  + H2S:  + Indole:  neg Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    erythromycin:  S(15; disc)
    ciprofloxacin:  S(5; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    oxytetracycline:  I(30; disc)
    bacitracin:  R(10U)

    References

    SPECIFIC REFERENCES FOR AMEDIBACILLUS DOLICHUS
  • Ikeyama2020 - Amedibacterium intestinale gen. nov., sp. nov., isolated from human faeces, and reclassification of Eubacterium dolichum Moore et al. 1976 (Approved Lists 1980) as Amedibacillus dolichus gen. nov., comb. nov.
  • Jackson2016 - Signatures of early frailty in the gut microbiota
  • Moore1976 - Emendation of Bacteroidaceae and Butyrivibrio and Descriptions of Desulfomonas gen. nov. and Ten New Species in the Genera Desulfomonas, Butyrivibrio, Eubacterium, Clostridium, and Ruminococcus.
  • Paek2017 - Description of Absiella argi gen. nov., sp. nov., and transfer of Eubacterium dolichum and Eubacterium tortuosum to the genus Absiella as Absiella dolichum comb. nov. and Absiella tortuosum comb. nov.
  • Schwiertz2000 - Quantification of Different Eubacterium spp. in Human Fecal Samples with Species-Specific 16S rRNA-Targeted Oligonucleotide Probes.
  • Wade2011Bergeys - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Eubacteriaceae, Genus I. Eubacterium
  • Margiotta2020 - Gut microbiota composition and frailty in elderly patients with Chronic Kidney Disease
  • Laue2020 - Prospective associations of the infant gut microbiome and microbial function with social behaviors related to autism at age 3 years
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR AMEDIBACILLUS DOLICHUS
  • Dubinkina2017 - Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • Jeong2021 - The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
  • PerezBrocal2015 - Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects Potential Interactions and Biomarkers
  • Wang2020a - Aberrant gut microbiota alters host metabolome and impacts renal failure in humans and rodents
  • Woodmansey2004 - Comparison of compositions and metabolic activities of fecal microbiotas in young adults and in antibiotic-treated and non-antibiotic-treated elderly subjects.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR AMEDIBACILLUS DOLICHUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: July 12, 2021