Anaerobiospirillum thomasii

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Anaerobiospirillum thomasii is a Gram-negative, non-spore-forming, strictly anaerobic, motile, spirochete bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 39-42%. Anaerobiospirillum thomasii is probably a rare gut coloniser. (Hippe1999; Malnick1997; Morotomi2010; Malnick2005Bergey)



  • This organism has been recovered from human faeces (CCUG, diarrhoea) and animals. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread (notes: human and animal pathogen). It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Hippe1999); (Malnick1997); (Morotomi2010); (Malnick2005Bergey);
    Character Response
  • 🌡
  • Temperature tolerance:
  • grows at 33℃; grows at 43℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • galactose; glucose; maltose; adonitol;
  • Active enzymes:
  • N-Ac β-glucosaminidase; Leu arylamidase;
    • SPECIAL FEATURES (Hippe1999); (Morotomi2010); (Malnick2005Bergey);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; succinate;
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Malnick2005Bergey); (Goldstein2006c); (Goldstein1999b);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; imipenem; meropenem; mezlocillin; penicillin G; piperacillin-tazobactam;
  • Cephalosporins:
  • cefotaxime; cefoxitin;
  • cefalexin;
  • Macrolides:
  • azithromycin;
  • roxithromycin;
  • Tetracyclines:
  • doxycycline; tigecycline;
  • Quinolines:
  • ciprofloxacin; gemifloxacin; moxifloxacin; trovafloxacin;
  • nalidixic-acid;
  • Heterocycles:
  • chloramphenicol;
  • sulfamethoxazole; trimethoprim;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; lincomycin; polymyxin B;
  • NOTES

    Anaerobiospirillum thomasii was originally isolated from human feces.

  • Malnick, H. (1997). Anaerobiospirillum thomasii sp. nov., an anaerobic spiral bacterium isolated from the feces of cats and dogs and from diarrheal feces of humans, and emendation of the genus Anaerobiospirillum. International Journal of Systematic Bacteriology, 47(2), 381–384.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Aeromonadales Family:  Succinivibrionaceae Genus:  Anaerobiospirillum Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Swimming Morphology:  Spirochete
    Health:  Unknown
    Source:  human faeces (CCUG, diarrhoea) and animals
    DNA G+C(%):  39-42
    Opt. T:  37℃
    Mid T(℃):  33(+)
    High T(℃):  43(+)
    		Aesculin:  neg Urea:  neg Hippurate:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  neg Galactose:  + Glucose:  + Cellubiose:  neg Lactose:  neg Maltose:  + Sucrose:  neg Trehalose:  neg Inulin:  neg Adonitol:  + Mannitol:  neg Sorbitol:  neg Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Ac-β-glcamnd:  + α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg Chymotrypsin:  neg Trypsin:  neg CystineAA:  neg LeuAA:  + ValAA:  neg AlkalineP:  neg AcidP:  neg Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 0.125, MIC90: 0.5, RNG: (0.125–0.5)
    ampicillin:  Var
    mezlocillin:  Sens
    penicillin_G:  S(MIC50): 0.06, MIC90: 0.5, RNG: (0.06–1)
    piper-taz:  S(MIC50): 0.125, MIC90: 4, RNG: (0.03–4)
    imipenem:  S(MIC50): 0.06, MIC90: 0.125, RNG: (0.06–0.125)
    meropenem:  S(MIC50): 0.03, MIC90: 0.03, RNG: (0.015–0.03)
    cefalexin:  Res
    cefotaxime:  S(MIC50): ≤0.015, MIC90: 0.06, RNG: (≤0.015–0.06)
    cefoxitin:  Sens
    azithromycin:  S(MIC50): 0.5, MIC90: 1, RNG: (0.125–1)
    erythromycin:  Var(MIC50): 4, MIC90: 16, RNG: (1–16)
    clarithromycin:  Var(MIC50): 4, MIC90: 32, RNG: (2–32)
    roxithromycin:  R(MIC50): 16, MIC90: >32, RNG: (4–>32)
    telithromycin:  Var(MIC50): 2, MIC90: 8, RNG: (0.5–8)
    ciprofloxacin:  Sens
    gemifloxacin:  S(MIC50): 0.125, MIC90: 0.125, RNG: (0.06–0.25)
    moxifloxacin:  S(MIC50): 0.125, MIC90: 0.5, RNG: (0.06–1)
    nalidixic-acid:  Res
    trovafloxacin:  S(MIC50): 0.125, MIC90: 1, RNG: (0.06–2)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.125–0.5)
    tigecycline:  S(MIC50): 0.06, MIC90: 0.125, RNG: (0.06–0.25)
    vancomycin:  Res
    chloramphenicol:  Sens
    metronidazole:  Var(MIC50): 4, MIC90: 8, RNG: (1–8)
    sulfamethoxazole:  Res
    trimethoprim:  Res
    clindamycin:  R(MIC50): 32, MIC90: >32, RNG: (8–>32)
    lincomycin:  Res
    polymyxin_B:  Res

    References

    SPECIFIC REFERENCES FOR ANAEROBIOSPIRILLUM THOMASII
  • Hippe1999 - Phylogenetic analysis of Formivibrio citricus, Propionivibrio dicarboxylicus, Anaerobiospirillum thomasii, Succinimonas amylolytica and Succinivibrio dextrinosolvens and proposal of Succinivibrionaceae fam. nov.
  • Malnick1997 - Anaerobiospirillum thomasii sp. nov., an Anaerobic Spiral Bacterium Isolated from the Feces of Cats and Dogs and from Diarrheal Feces of Humans, and Emendation of the Genus Anaerobiospirillum.
  • Morotomi2010 - Succinatimonas hippei gen. nov., sp. nov., isolated from human faeces.
  • Malnick2005Bergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Succinivibrionaceae, Genus II. Anaerobiospirillum
  • Goldstein2006c - Comparative in vitro susceptibilities of 396 unusual anaerobic strains to tigecycline and eight other antimicrobial agents.
  • Goldstein1999b - Activities of telithromycin (HMR 3647, RU 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and other antimicrobial agents against unusual anaerobes.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ANAEROBIOSPIRILLUM THOMASII
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR ANAEROBIOSPIRILLUM THOMASII
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: April 08, 2021