Aneurinibacillus aneurinilyticus

(aka Bacillus aneurinilyticus)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Aneurinibacillus aneurinilyticus, (aka Bacillus aneurinilyticus), is a Gram-positive, spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 42.9%. Aneurinibacillus aneurinilyticus is probably a rare gut coloniser. (Heyndrickx1997; Shida1996; Subhash2017; Logan2011aBergey)



  • This organism has been recovered from human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Heyndrickx1997); (Shida1996); (Subhash2017); (Logan2011aBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • starch; tyrosine;
  • 🧂
  • Salt tolerance:
  • tolerates 0% salt; grows weakly in 7.0% salt; Grows optimally with 3-4% salt.
  • pH
  • Acidity tolerance:
  • tolerates pH 5.0; tolerates pH 9.0; Grows optimally at pH 6.5-7.
  • 🌡
  • Temperature tolerance:
  • strain-variable at 20(d); grows at 35℃; strain-variable at 50(d); Grows optimally at 35-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; mannose; maltose; melezitose; melibiose; sucrose;
  • ⥅
  • Substrates assimilated or utilised:
  • ethanolamine; glycerol; mannose; melezitose; melibiose; xylose; δ-aminovalerate; proline; fumarate; glutarate; 2-ketogluconate; DL-lactate; malonate; propionate; succinate; putrescine;
  • ±
  • Strain-dependent substrate utilisation:
  • N-acetylglucosamine; adonitol; cellubiose; gluconate; lactose; ribose; D-tagatose; trehalose; alanine; aspartate; serine; 2-ketoglutarate; L-malate; phenylacetate; D-tartrate; methyl-β-glucoside; D-malate;
  • Active enzymes:
  • chymotrypsin; cystine arylamidase; esterase C4; esterase lipase C8; Leu arylamidase; oxidase; trypsin; Val arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Heyndrickx1997); (Subhash2017); (Logan2011aBergey);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • Pigments:
  • pale yellow
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Penicillins:
  • penicillin;
  • ampicillin;
  • Quinolines:
  • nalidixic-acid;
  • Heterocycles:
  • chloramphenicol;
  • NOTES

    Aneurinibacillus aneurinilyticus was isolated from human feces.  Like A. migulanus, A. aneurinilyticus can utilise both hydroxyquinoline-b-glucuronide and octanoate.

  • Shida, O., Takagi, H., Kadowaki, K., Yano, H., Abe, M., Udaka, S., & Komagata, K. (1994). Bacillus aneurinolyticus sp. nov., nom. rev. International Journal of Systematic Bacteriology, 44(1), 143–150.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Paenibacillaceae Genus:  Aneurinibacillus Alt. name:  Bacillus aneurinilyticus Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod Pigment:  pale yellow
    Health:  Unknown
    Source:  human faeces
    DNA G+C(%):  42.9
    Opt. T:  35-37℃
    Lower T(℃):  20(d)
    Mid T(℃):  35(+)
    High T(℃):  50(d)
    NaCl 0.5-2%:  0(+)
    NaCl >6%:  7.0(w)
    Opt. pH:  6.5-7
    pH 4.2-5.9:  5.0(+)
    pH >8:  9.0(+)
    		Aesculin:  neg Urea:  neg Gelatin:  neg Starch:  + Casein:  neg Tyrosine:  + Tween:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg Fructose:  neg Fucose:  neg Galactose:  neg Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  neg Sorbose:  neg L-Sorbose:  neg D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  + Melezitose:  + Melibiose:  + Sucrose:  + Trehalose:  neg Adonitol:  neg Dulcitol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg NAc-α-GA:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  neg Fucose:  neg Galactose:  neg Glucose:  neg D-Lyxose:  neg Mannose:  + Rhamnose:  neg Ribose:  d Sorbose:  neg D-Tagatose:  d Xylose:  + Cellubiose:  d Gentiobiose:  neg Lactose:  d Maltose:  neg Maltotriose:  neg Melezitose:  + Melibiose:  + Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  d N_Acetyl_glucosamine:  d Adonitol:  d D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Ethanolamine:  + Gluconate:  d Glucosamine:  neg Glycerol:  + Inositol:  neg Mannitol:  neg Me-α-D-Glc:  neg Sorbitol:  neg Xylitol:  neg Ala:  d D-Ala:  neg 4-Aminobutyrate:  neg 5-Aminovalerate:  + Asp:  d Betaine:  neg Glu:  neg Histamine:  neg His:  neg Pro:  + Ser:  d Trigonelline:  neg Tryptamine:  neg Trp:  neg Tyr:  neg Aconitate:  neg Benzoate:  neg Caprate:  neg Caprylate:  neg Citrate:  neg Fumarate:  + Glutarate:  + DL-Glycerate:  neg 3-Hydroxybutyrate:  neg 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Itaconate:  neg 2-Ketogluconate:  + 5-Ketogluconate:  neg DL-Lactate:  + L-Malate:  d Malonate:  + Mucate:  neg Phenylacetate:  d Phenylpropionate:  neg Propionate:  + Quinate:  neg Saccharate:  neg Succinate:  + D-Tartrate:  d L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  w Urease:  neg Ac-β-glcamnd:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg ArgDC:  neg Chymotrypsin:  + LysDC:  neg Trypsin:  + CystineAA:  + LeuAA:  + ValAA:  + AlkalineP:  neg AcidP:  neg Esterase(C4):  + EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  pale yellow
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  Res
    penicillin:  Sens
    nalidixic-acid:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    chloramphenicol:  Sens

    References

    SPECIFIC REFERENCES FOR ANEURINIBACILLUS ANEURINILYTICUS
  • Heyndrickx1997 - A Polyphasic Reassessment of the Genus Aneurinibacillus, Reclassification of Bacillus thermoaerophilus (Meier-Stauffer et al. 1996) as Aneurinibacillus thermoaerophilus comb, nov., and Emended Descriptions of A. aneurinilyticus corrig., A. migulanus, and A. thermoaerophilus.
  • Shida1996 - Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.
  • Subhash2017 - Aneurinibacillus sediminis sp. nov., isolated from lagoon sediments.
  • Logan2011aBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Paenibacillaceae, Genus III. Aneurinibacillus
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ANEURINIBACILLUS ANEURINILYTICUS
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR ANEURINIBACILLUS ANEURINILYTICUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021