Aneurinibacillus migulanus

(aka Bacillus migulanus)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Aneurinibacillus migulanus, (aka Bacillus migulanus), is a Gram-positive, spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 42.5%. Aneurinibacillus migulanus is probably a rare gut coloniser. (Heyndrickx1997; Shida1996; Logan2011aBergey; Takagi1993)



  • This organism has been recovered from human faeces and garden soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Heyndrickx1997); (Shida1996); (Logan2011aBergey); (Takagi1993);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin; starch;
  • 🧂
  • Salt tolerance:
  • tolerates 2.0% salt; tolerates 5.0% salt; grows weakly in 7.0% salt;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.0; tolerates pH 6.0-8.0; tolerates pH >9.0;
  • 🌡
  • Temperature tolerance:
  • grows at 20℃; grows at 48℃; Grows optimally at 30-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; inositol;
  • ⥅
  • Substrates assimilated or utilised:
  • ethanolamine; fructose; gluconate; glycerol; ribose; alanine; D-alanine; γ-aminobutyrate; δ-aminovalerate; aspartate; glutamate; proline; serine; trigonelline; tryptophan; tyrosine; fumarate; 3-hydroxybutyrate; 3-hydroxybenzoate; 2-ketogluconate; 5-ketogluconate; DL-lactate; malate; malonate; phenylacetate; propionate; succinate; glutamine;
  • ±
  • Strain-dependent substrate utilisation:
  • inositol; histamine; histidine; aconitate; glutarate; 2-ketoglutarate; L-malate;
  • Active enzymes:
  • catalase; oxidase;
    • SPECIAL FEATURES (Heyndrickx1997); (Logan2011aBergey); (Takagi1993);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Takagi1993);
    Class Active Resistant
  • Penicillins:
  • penicillin;
  • ampicillin;
  • Quinolines:
  • nalidixic-acid;
  • Heterocycles:
  • chloramphenicol;
  • NOTES

    Weakly utilises both trans-aconitate and cis-aconitate. Like A. aneurinilyticus, A. migulanus can utilise hydroxyquinoline-b-glucuronide and octanoate. Unlike its relative, this organism can also use protocatechuate: https://opsin.ch.cam.ac.uk/opsin/Protocatechuic%20acid.png

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Paenibacillaceae Genus:  Aneurinibacillus Alt. name:  Bacillus migulanus Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and garden soil
    DNA G+C(%):  42.5
    Opt. T:  30-37℃
    Lower T(℃):  20(+)
    High T(℃):  48(+)
    NaCl 0.5-2%:  2.0(+)
    NaCl 3-5%:  5.0(+)
    NaCl >6%:  7.0(w)
    pH 4.2-5.9:  5.0(neg)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  >9.0(+)
    		Gelatin:  + Starch:  + Casein:  neg Tween:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg Fructose:  + Glucose:  neg Mannose:  neg Sorbose:  neg L-Sorbose:  neg D-Tagatose:  neg Xylose:  neg Adonitol:  neg Dulcitol:  neg Inositol:  + Sorbitol:  neg Xylitol:  neg NAc-α-GA:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  + Fucose:  neg Galactose:  neg Glucose:  neg D-Lyxose:  neg Mannose:  neg Ribose:  + D-Tagatose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Maltotriose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg N_Acetyl_glucosamine:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Erythritol:  neg Aesculin:  neg Ethanolamine:  + Gluconate:  + Glucosamine:  neg Glycerol:  + Inositol:  d Mannitol:  neg Me-α-D-Glc:  neg Xylitol:  neg Ala:  + D-Ala:  + 4-Aminobutyrate:  + 5-Aminovalerate:  + Asp:  + Betaine:  neg Glu:  + Histamine:  d His:  d Pro:  + Ser:  + Trigonelline:  + Tryptamine:  neg Trp:  + Tyr:  + Aconitate:  d Benzoate:  neg Caprate:  neg Caprylate:  neg Citrate:  neg Fumarate:  + Glutarate:  d DL-Glycerate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  + 4-Hydroxybenzoate:  neg Itaconate:  neg 2-Ketogluconate:  + 5-Ketogluconate:  + DL-Lactate:  + Malate:  + L-Malate:  d Malonate:  + Mucate:  neg Phenylacetate:  + Phenylpropionate:  neg Propionate:  + Quinate:  neg Saccharate:  neg Succinate:  + D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  Res
    penicillin:  Sens
    nalidixic-acid:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    chloramphenicol:  Sens

    References

    SPECIFIC REFERENCES FOR ANEURINIBACILLUS MIGULANUS
  • Heyndrickx1997 - A Polyphasic Reassessment of the Genus Aneurinibacillus, Reclassification of Bacillus thermoaerophilus (Meier-Stauffer et al. 1996) as Aneurinibacillus thermoaerophilus comb, nov., and Emended Descriptions of A. aneurinilyticus corrig., A. migulanus, and A. thermoaerophilus.
  • Shida1996 - Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.
  • Logan2011aBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Paenibacillaceae, Genus III. Aneurinibacillus
  • Takagi1993 - Characterization of Bacillus brevis with Descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ANEURINIBACILLUS MIGULANUS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR ANEURINIBACILLUS MIGULANUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021