Bacillus endophyticus

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bacillus endophyticus is a Gram-positive, spore-forming, strictly aerobic, non-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. Bacillus endophyticus is likely to be transient and not a long-term gut coloniser. (Logan2011Bergey; Reva2002)



  • This organism has been recovered from plants and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal. Robust growth can have positive consequences for gut health.
    • QUIRKS
  • Borderline cold mesophile, with optimum growth temp of 28C. Unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Logan2011Bergey); (Reva2002);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 2.0% salt; tolerates 5.0% salt; tolerates 10% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 7.0;
  • 🌡
  • Temperature tolerance:
  • grows at 0-5(neg), 10℃; grows at 40℃; doesn't grow at 50℃; Grows optimally at 28℃.
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; glucose; mannose; rhamnose; ribose; xylose; maltose; melibiose; raffinose; sucrose; inositol; mannitol;
  • ⥅
  • Substrates assimilated or utilised:
  • gluconate; melibiose; citrate;
  • Active enzymes:
  • catalase; β-galactosidase; oxidase;
    • SPECIAL FEATURES (Logan2011Bergey); (Reva2002);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • VP test:
  • not active
  • ✓
  • ONPG test:
  • active
  • Lysozyme:
  • growth observed (1000)
  • ✗
  • Nitrate:
  • not reduced
  • ✓
  • Pigments:
  • produced
    • RESPONSE TO ANTIBIOTICS (Reva2002);
    Class Active Resistant
  • Penicillins:
  • ampicillin;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Bacillus Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Sessile Morphology:  Rod Pigment:  +
    Health:   Positive
    Source:  plants and human faeces
    Opt. T:  28℃
    Low T(℃):  0-5(neg), 10(+)
    Mid T(℃):  40(+)
    High T(℃):  50(neg)
    NaCl 0.5-2%:  2.0(+)
    NaCl 3-5%:  5.0(+)
    NaCl >6%:  10(+)
    pH 6.0-8.0:  7.0(+)
    		Urea:  neg Gelatin:  neg Starch:  neg Casein:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  + Galactose:  neg Glucose:  + Mannose:  + Rhamnose:  + Ribose:  + Xylose:  d(+) Cellubiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  + Sucrose:  + Trehalose:  neg Glycogen:  neg Adonitol:  neg Glycerol:  neg Inositol:  + Mannitol:  + Sorbitol:  neg Me-α-D-Mann:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  + Gluconate:  + Acetate:  neg Citrate:  + Propionate:  neg D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + β-Galactosidase:  + ArgDH:  neg Phe deaminase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg Pigment:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  R(100)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)

    References

    SPECIFIC REFERENCES FOR BACILLUS ENDOPHYTICUS
  • Logan2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Bacillaceae Genus I. Bacillus
  • Reva2002 - Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.).
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BACILLUS ENDOPHYTICUS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR BACILLUS ENDOPHYTICUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021