Bacillus methanolicus

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bacillus methanolicus is a Gram-positive, spore-forming, strictly aerobic, non-motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 48-50%. Bacillus methanolicus is unlikely to be a gut coloniser. (Logan2011Bergey; Arfman1992)



  • This organism has been recovered from soil, wastewater treatment systems and hotsprings. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe and high salt required for optimum growth, so likely a gut non-coloniser.
    • GENERAL CHARACTERISTICS (Logan2011Bergey); (Arfman1992);
    Character Response
  • ±
  • Strain-dependent hydrolysis or digestion:
  • starch;
  • 🧂
  • Salt tolerance:
  • strain-variable at 2.0(d)%; doesn't tolerate 5.0% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0(neg), 7.0;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 30℃; grows at 35℃; grows at 55℃; Grows optimally at 55℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; maltose; raffinose; trehalose; mannitol; sorbitol;
  • ±
  • Strain-dependent acid from carbs:
  • ribose;
  • ⥅
  • Substrates assimilated or utilised:
  • mannitol; methanol;
  • ±
  • Strain-dependent substrate utilisation:
  • ethanol; acetate; pyruvate;
  • Active enzymes:
  • catalase; oxidase;
    • SPECIAL FEATURES (Logan2011Bergey); (Arfman1992);
    Character Response
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Arfman1992);
    Class Active Resistant
  • Penicillins:
  • ampicillin; cloxacillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • gentamicin; kanamycin; neomycin; streptomycin;
  • Heterocycles:
  • chloramphenicol; fusidic-acid; trimethoprim;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • novobiocin; polymyxin B;

  • N/A

  • Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value-added products. Its capabilities of producing and secreting the commercially important amino acids l-glutamate and l-lysine to high concentrations. [PMID: 25431011]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    Bacillus methanolicus MGA3 was isolated from freshwater marsh soil and characterised as a thermotolerant and methylotrophic L-glutamate producer. The complete genome consists of a circular chromosome and the two plasmids pBM19 and pBM69. It includes genomic information about C1 metabolism and amino acid biosynthetic pathways. [PMID: 25152427]

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Bacillus Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  soil, wastewater treatment systems and hotsprings
    DNA G+C(%):  48-50
    Opt. T:  55℃
    Lower T(℃):  30(neg)
    Mid T(℃):  35(+)
    High T(℃):  55(+)
    NaCl 0.5-2%:  2.0(d)
    NaCl 3-5%:  5.0(neg)
    pH 6.0-8.0:  6.0(neg), 7.0(+)
    		Starch:  d Casein:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  d(+) Galactose:  neg Glucose:  + Ribose:  d L-Xylose:  neg Lactose:  neg Maltose:  d(+) Sucrose:  neg Trehalose:  d(+) Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  + Sorbitol:  d(+) NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Ethanol:  d Mannitol:  + Methanol:  + Hippurate:  neg Acetate:  d Pyruvate:  d Succinate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(10; disc)
    cloxacillin:  S(5; disc)
    gentamicin:  S(10; disc)
    kanamycin:  S(5; disc)
    neomycin:  S(10; disc)
    streptomycin:  S(10; disc)
    erythromycin:  S(10; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(10; disc)
    vancomycin:  S(5; disc)
    chloramphenicol:  S(30; disc)
    trimethoprim:  S(5; disc)
    novobiocin:  S(5; disc)
    polymyxin_B:  S(300; disc)
    fusidic-acid:  S(10; disc)

    References

    SPECIFIC REFERENCES FOR BACILLUS METHANOLICUS
  • Logan2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Bacillaceae Genus I. Bacillus
  • Arfman1992 - Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BACILLUS METHANOLICUS
  • King2019 - Baseline human gut microbiota profile in healthy people and standard reporting template.
  • ...............................
    • GENERAL REFERENCES FOR BACILLUS METHANOLICUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: February 08, 2021