Bacillus smithii

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bacillus smithii is a Gram-positive, spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 38.7-39.7%. Bacillus smithii is probably a rare gut coloniser. (Nakamura1988)



  • This organism has been recovered from food and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Nakamura1988);
    Character Response
  • Substrates hydrolysed or digested:
  • DNA; hippurate;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • starch;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 7% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 5.7;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 60℃;
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; ribose; xylose; aesculin; maltose; trehalose; mannitol;
  • ±
  • Strain-dependent acid from carbs:
  • arabinose; L-arabinose; mannose; rhamnose; glycerol; sorbitol;
  • ⥅
  • Substrates assimilated or utilised:
  • acetate; citrate; fumarate; malate; succinate;
  • ±
  • Strain-dependent substrate utilisation:
  • propionate;
  • Active enzymes:
  • oxidase;
    • SPECIAL FEATURES (Nakamura1988);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S; indole;
  • ±
  • VP test:
  • active for a few strains
  • ✗
  • ONPG test:
  • not active
  • ✗
  • NO3➔NO2:
  • not reduced

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Bacillus Gram stain:  + O2 Relation.:  facultatively anaerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  food and human faeces
    DNA G+C(%):  38.7-39.7
    Lower T(℃):  25(+)
    High T(℃):  60(+)
    NaCl >6%:  7(neg)
    pH 4.2-5.9:  5.7(+)
    		Aesculin:  vr Urea:  neg Gelatin:  neg Starch:  w(d) Casein:  neg Chitin:  neg DNA:  + Tyrosine:  neg Hippurate:  + Milk:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  d D-Arabinose:  neg L-Arabinose:  d(neg) Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + Mannose:  d(neg) D-Lyxose:  neg Rhamnose:  d(neg) Ribose:  d(+) Sorbose:  neg D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  d(+) Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  + Turanose:  neg Amygdalin:  neg Aesculin:  d(+) Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  d Inositol:  neg Mannitol:  d(+) Sorbitol:  d(neg) Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Acetate:  + Citrate:  + Fumarate:  + Malate:  + Propionate:  d Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Urease:  neg β-Galactosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg TrpAA:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg

    References

    SPECIFIC REFERENCES FOR BACILLUS SMITHII
  • Nakamura1988 - Taxonomic Study of Bacillus coagulans Hammer 1915 with a Proposal for Bacillus smithii sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BACILLUS SMITHII
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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    • Added: December 15, 2021