Bacteroides cellulosilyticus

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bacteroides cellulosilyticus is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 22 gut microbiome compilation studies or metastudies. The DNA G+C content is 41.1%. Bacteroides cellulosilyticus is a common gut coloniser. (Robert2007)



  • This organism has been recovered from human faeces (CCUG) and clinical sources (blood - CCUG). The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Is a rare opportunistic pathogen. Is a known gut commensal. Robust growth can have mixed consequences for gut health.
    • QUIRKS
  • A common gut microbe and one that digests cellulose.
    • GENERAL CHARACTERISTICS (Robert2007);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; pectin; starch; cellulose;
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 39℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; mannose; rhamnose; xylose; cellulose; pectin; starch; maltose; sucrose;
  • ⥅
  • Substrates assimilated or utilised:
  • lactulose;
  • Active enzymes:
  • Ala arylamidase; alkaline phosphatase; acid phosphatase; N-Ac β-glucosaminidase; esterase C4; α-galactosidase; β-galactosidase; α-glucosidase; β-glucosidase; glutamic acid decarboxylase; Glu-Glu arylamidase; Leu-Gly arylamidase; xylosidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Robert2007);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate; succinate; indole;
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • brown
    • RESPONSE TO ANTIBIOTICS (Goldstein2013b);
    Class Active Resistant
  • Penicillins:
  • meropenem; piperacillin-tazobactam;
  • Cephalosporins:
  • ceftazidime;
  • Heterocycles:
  • metronidazole;
  • NOTES

    This is a common gut inhabitant.

    Fuel sources used:
    It can use numerous types of fibre, as well as resistant starch, simple sugars, protein and mucus for energy.

    Metabolites produced:
    Our genomic analysis indicates that most members of this species can produce the following metabolites: acetate, B-glucuronidase, BCAAs, butyrate, GABA, lactate, LPS, propionate, succinate, cobalamin, folate, biotin, riboflavin.

    Metabolites consumed:
    In addition, our genomic analysis indicates that most members of this species do not consume any reported metabolites.

    Disease associations:
    Elevated levels have been observed in patients with hypertension.

  • References: [1] [2] [3] [4]

  • This organism contains many genes invovled in carbohydrate utilization. [PMID: 23976882]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    B. cellulosilyticus WH2 is an adaptive forager that tailors its versatile carbohydrate utilization strategy to available dietary polysaccharides, with a strong emphasis on plant-derived xylans abundant in dietary staples like cereal grains. [PMID: 23976882]

  • Robert, C., & Bernalier-Donadille, A. (2003). The cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects. FEMS Microbiology Ecology, 46(1), 81–89.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Bacteroidia Order:  Bacteroidales Family:  Bacteroidaceae Genus:  Bacteroides Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod Pigment:  brown
    Health:  Mixed
    Source:  human faeces (CCUG) and clinical sources (blood - CCUG)
    DNA G+C(%):  41.1
    Opt. T:  37℃
    Lower T(℃):  30(+)
    Mid T(℃):  39(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Starch:  + Pectin:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  w Fucose:  neg D-Fucose:  neg Glucose:  + Mannose:  + Rhamnose:  + Xylose:  + Cellubiose:  w Lactose:  w Maltose:  + Melezitose:  neg Sucrose:  + Trehalose:  neg Cellulose:  + Aesculin:  w Starch:  + Pectin:  + Xylan:  w Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Salicin:  w
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg Ac-β-glcamnd:  + α-Fucosidase:  w α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg α-Mannosidase:  neg Xylosidase:  + ArgDH:  neg GluDC:  + AlanineAA:  + GluGluAA:  + GlyAA:  neg LeuAA:  neg LeuGlyAA:  + PyrrolidAA:  neg PheAA:  w TyrAA:  w AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  w Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    Fibre, Resistant Starch, Mucus, Simple Sugars, Protein

    None/Unknown

    Branched-Chain AA, Cobalamin, Folate, Biotin, Riboflavin, Acetate, Lactate, Propionate, Butyrate, Succinate, GABA

    Beta-glucuronidase, LPS Antigen

    		 Acetate:  + Propionate:  + Succinate:  + Indole:  + Pigment:  brown
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amp-sulb:  Var(MIC50): 4, MIC90: 16, RNG: (1–32)
    piper-taz:  S(MIC50): 2, MIC90: 8, RNG: (0.06–16)
    meropenem:  S(MIC50): 0.5, MIC90: 1, RNG: (0.125–32)
    cefoxitin:  Var(MIC50): 16, MIC90: 64, RNG: (4–64)
    ceftazidime:  R(MIC50): >128, MIC90: >128, RNG: (16–>128)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tigecycline:  Var(MIC50): 0.5, MIC90: 8, RNG: (0.125–32)
    metronidazole:  S(MIC50): 2, MIC90: 2, RNG: (0.5–2)
    clindamycin:  Var(MIC50): 2, MIC90: >128, RNG: (0.06–>128)

    References

    SPECIFIC REFERENCES FOR BACTEROIDES CELLULOSILYTICUS
  • Robert2007 - Bacteroides cellulosilyticus sp. nov., a cellulolytic bacterium from the human gut microbial community.
  • Gryp2020 - Isolation and Quantification of Uremic Toxin Precursor-Generating Gut Bacteria in Chronic Kidney Disease Patients
  • Kim2020a - Altered Gut Microbiome Profile in Patients With Pulmonary Arterial Hypertension
  • Tarallo2019 - Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
  • Zhang2019a - Fecal microbiota in patients with ankylosing spondylitis: Correlation with dietary factors and disease activity
  • Goldstein2013b - Comparative in vitro activities of GSK2251052, a novel boron-containing leucyl-tRNA synthetase inhibitor, against 916 anaerobic organisms.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BACTEROIDES CELLULOSILYTICUS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Chen2020 - Structural and Functional Characterization of the Gut Microbiota in Elderly Women With Migraine
  • Chen2020a - Featured Gut Microbiomes Associated With the Progression of Chronic Hepatitis B Disease
  • Dubinkina2017 - Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • Jeong2021 - The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
  • Jie2017 - The gut microbiome in atherosclerotic cardiovascular disease
  • Karlsson2013 - Gut metagenome in European women with normal, impaired and diabetic glucose control
  • King2019 - Baseline human gut microbiota profile in healthy people and standard reporting template.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • Nielsen2014 - MetaHIT Consortium. Identification and assembly of genomes and genetic elements in complex metagenomic samples without using reference genomes.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Rothschild2018 - Environment dominates over host genetics in shaping human gut microbiota.
  • Tyakht2013 - Human gut microbiota community structures in urban and rural populations in Russia.
  • Urban2020 - Altered Fecal Microbiome Years after Traumatic Brain Injury
  • Wang2020a - Aberrant gut microbiota alters host metabolome and impacts renal failure in humans and rodents
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zou2019 - 1,520 reference genomes from cultivated human gut bacteria enable functional microbiome analyses.
  • ...............................
    • GENERAL REFERENCES FOR BACTEROIDES CELLULOSILYTICUS
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: February 08, 2021