Bifidobacterium ruminantium

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bifidobacterium ruminantium is a Gram-positive, non-spore-forming, anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 57%. Bifidobacterium ruminantium is probably a common, although minor, coloniser of the gut. (Biavati1991)



  • This organism has been recovered from human faeces, and rumen of cattle. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • In 70% of Europeans (unseenbio.com). Minor coloniser.
    • GENERAL CHARACTERISTICS (Biavati1991);
    Character Response
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.9; grows weakly at pH 6.3 7.2; doesn't tolerate pH >8; Grows optimally at pH 6.5-6.9.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 15℃; grows at 25℃; grows at 45℃; Grows optimally at 38-42℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; ribose; dextrin; glycogen; starch; maltose; melibiose; raffinose; sucrose; mannitol; arbutin; α-methyl glucoside;
  • ⥅
  • Substrates assimilated or utilised:
  • melibiose;
    • SPECIAL FEATURES (Biavati1991);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate (major); lactate;
  • ✗
  • Metabolites not produced:
  • propionate; butyrate; indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Goldstein2013);
    Class Active Resistant
  • Penicillins:
  • imipenem; piperacillin-tazobactam;
  • Heterocycles:
  • chloramphenicol;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Bifidobacteriales Family:  Bifidobacteriaceae Genus:  Bifidobacterium Gram stain:  + O2 Relation.:  anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, and rumen of cattle
    DNA G+C(%):  57
    Opt. T:  38-42℃
    Low T(℃):  15(neg)
    Lower T(℃):  25(+)
    High T(℃):  45(+)
    Opt. pH:  6.5-6.9
    pH 4.2-5.9:  5.9(neg)
    pH 6.0-8.0:  6.3(w) 7.2(w)
    pH >8:  >8(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  neg D-Lyxose:  neg Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  vr Maltose:  + Melezitose:  neg Melibiose:  + Sucrose:  + Trehalose:  neg Turanose:  neg Amygdalin:  neg Dextrin:  + Glycogen:  + Inulin:  neg Starch:  + Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  + Sorbitol:  neg Xylitol:  neg Arbutin:  + Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  + Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  vr
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  + Lactate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  Major(+) Propionate:  neg Butyrate:  neg Lactate:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    piper-taz:  S(MIC50): 0.06, MIC90: 1, RNG: (0.015–2)
    imipenem:  S(MIC50): 0.06, MIC90: 0.5, RNG: (0.015–2)
    moxifloxacin:  Var(MIC50): 1, MIC90: 8, RNG: (0.06–16)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    chloramphenicol:  Sens
    metronidazole:  Var(MIC50): 16, MIC90: >32, RNG: (0.5–>32)

    References

    SPECIFIC REFERENCES FOR BIFIDOBACTERIUM RUMINANTIUM
  • Biavati1991 - Bifidobacterium ruminantium sp. nov. and Bifidobacterium merycicum sp. nov. from the Rumens of Cattle.
  • Goldstein2013 - In vitro activity of Biapenem plus RPX7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BIFIDOBACTERIUM RUMINANTIUM
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • Added: April 08, 2021