Bifidobacterium thermophilum

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bifidobacterium thermophilum is a Gram-positive, non-spore-forming, microaerophilic, non-motile, rod-shaped bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 59.7%. Bifidobacterium thermophilum is probably a rare gut coloniser. (Ah2007; Scardovi1971)



  • This organism has been recovered from human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal. Robust growth can have positive consequences for gut health.
    • GENERAL CHARACTERISTICS (Ah2007); (Scardovi1971);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • tolerates 4.5% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 47℃;
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; starch; maltose; raffinose; sucrose;
  • ±
  • Strain-dependent acid from carbs:
  • aesculin; α-methyl glucoside;
  • ⥅
  • Substrates assimilated or utilised:
  • melibiose;
    • SPECIAL FEATURES (Ah2007); (Scardovi1971);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; lactate;
  • ✗
  • Metabolites not produced:
  • indole;
    • RESPONSE TO ANTIBIOTICS (Kheadr2007); (Matteuzzi1983);
    Class Active Resistant
  • Penicillins:
  • ampicillin; penicillin G;
  • cloxacillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • kanamycin; neomycin;
  • Polypep/ketides:
  • bacitracin; nisin A;
  • rifampicin;
  • Heterocycles:
  • chloramphenicol; nitrofurantoin;
  • Miscellaneous antibiotics:
  • novobiocin; polymyxin B;

  • N/A

  • RBL67 is a moderately oxygen-tolerant strain that reaches high cell numbers in fermentation. Furthermore, it produces a bacteriocin-like inhibitory substance (BLIS) that is active against Listeria spp. [PMID: 23640377]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    The infant feces isolate B. thermophilum RBL67 (RBL67) is a promising probiotic candidate. The strain can grow under low oxygen, at pHs as low as 4.0 and at temperatures up to 47 ��C. [PMID: 26988691]

  • Finegold, S. M., Howard, R. A., & Vera, L. S. (1974). Effect of diet on human intestinal fecal flora: comparison of Japanese and American diets. Am. J. Clin. Nutr, 27, 1456–1469.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Bifidobacteriales Family:  Bifidobacteriaceae Genus:  Bifidobacterium Gram stain:  + O2 Relation.:  microaerophilic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:   Positive
    Source:  human faeces
    DNA G+C(%):  59.7
    Lower T(℃):  25(+)
    High T(℃):  47(+)
    NaCl 3-5%:  4.5(+)
    		Aesculin:  + Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  neg D-Lyxose:  neg Rhamnose:  neg Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Sucrose:  + Trehalose:  neg Turanose:  neg Amygdalin:  neg Aesculin:  d Glycogen:  neg Inulin:  w Starch:  + Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  d Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    Bacteriocin

    		 Acetate:  + Lactate:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(<0.98)
    cloxacillin:  R(15.6)
    penicillin_G:  S(MIC50): 0.16, MIC90: 0.27, RNG: (0.1-0.3)
    kanamycin:  R(MIC50): 130, MIC90: 170, RNG: (100-200)
    neomycin:  R(>400)
    streptomycin:  Var(MIC50): 39, MIC90: 89, RNG: 30-100)
    erythromycin:  SensRNG: (0.98-1.95)
    nalidixic-acid:  R(125)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  R(MIC50): 139, MIC90: 238, RNG: (50-250)
    bacitracin:  S(MIC50): 0.13, MIC90: 0.6, RNG: (0.05-1.5)
    nisin_A:  Sens
    rifampicin:  R(>32)
    chloramphenicol:  S(MIC50): 2.3, MIC90: 3.5, RNG: (2-4)
    nitrofurantoin:  S(MIC50): <15, MIC90: 21, RNG: (15-30)
    novobiocin:  R(>32)
    polymyxin_B:  R(>700)

    References

    SPECIFIC REFERENCES FOR BIFIDOBACTERIUM THERMOPHILUM
  • Ah2007 - Classification of a moderately oxygen-tolerant isolate from baby faeces as Bifidobacterium thermophilum.
  • Kheadr2007 - Antibiotic Susceptibility Profile of Bifidobacteria as Affected by Oxgall, Acid, and Hydrogen Peroxide Stress.
  • Matteuzzi1983 - Antimicrobial susceptibility of Bifidobacterium.
  • Scardovi1971 - Deoxyribonucleic Acid Homology Relationships Among Species of the Genus Bifidobacterium
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BIFIDOBACTERIUM THERMOPHILUM
  • King2019 - Baseline human gut microbiota profile in healthy people and standard reporting template.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • Added: February 08, 2021