Bradyrhizobium denitrificans

(aka Blastobacter denitrificans)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Bradyrhizobium denitrificans, (aka Blastobacter denitrificans), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. Bradyrhizobium denitrificans is probably a rare gut coloniser. (vanBerkum2006; Trotha2002)



  • This organism has been recovered from human faeces, plant endosymbiotic, and clinical sources. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (vanBerkum2006); (Trotha2002);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; urea;
  • ⥅
  • Substrates assimilated or utilised:
  • fructose; galactose; glucose; glycerol; lactose; mannitol; ribose; glutamate; glutarate; pyruvate; succinate; glutamine; oxoglutarate;
  • ±
  • Strain-dependent substrate utilisation:
  • N-acetylglucosamine; ethanol; maltose; methanol; sucrose; glycine; hippurate; leucine; proline; acetate; citrate; formate; fumarate; malate; D-tartrate; L-tartrate;
  • Active enzymes:
  • catalase; oxidase;
    • SPECIAL FEATURES (vanBerkum2006);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrite:
  • not reduced
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Trotha2002);
    Class Active Resistant
  • Penicillins:
  • imipenem;

  • Bik, E. M., Eckburg, P. B., Gill, S. R., Nelson, K. E., Purdom, E. A., Francois, F., Perez-Perez, G., Blaser, M. J., & Relman, D. A. (2006). Molecular analysis of the bacterial microbiota in the human stomach. Proceedings of the National Academy of Sciences of the United States of America, 103(3), 732–737.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Bradyrhizobiaceae Genus:  Bradyrhizobium Alt. name:  Blastobacter denitrificans Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, plant endosymbiotic, and clinical sources
    		Aesculin:  + Urea:  + Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Fructose:  + Galactose:  + Glucose:  + Ribose:  + Cellubiose:  neg Lactose:  + Maltose:  d Sucrose:  d N_Acetyl_glucosamine:  d Dextrin:  neg Ethanol:  d Glycerol:  + Inulin:  neg Mannitol:  + Methanol:  d Salicin:  neg Starch:  neg Ala:  neg Arg:  neg Asp:  neg Glu:  + Gly:  d Hippurate:  d Leu:  d Phe:  neg Pro:  d Ser:  neg Acetate:  d Caproate:  neg Citrate:  d Formate:  d Fumarate:  d Glutarate:  + Lactate:  neg Malate:  d Propionate:  neg Pyruvate:  + Succinate:  + D-Tartrate:  d L-Tartrate:  d
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  vr β-Galactosidase:  neg ArgDH:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    imipenem:  S(clinic)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)

    References

    SPECIFIC REFERENCES FOR BRADYRHIZOBIUM DENITRIFICANS
  • vanBerkum2006 - Proposal for combining Bradyrhizobium spp. (Aeschynomene indica) with Blastobacter denitrificans and to transfer Blastobacter denitrificans (Hirsch and Muller, 1985) to the genus Bradyrhizobium as Bradyrhizobium denitrificans (comb. nov.).
  • Hugenholtz1994 - A Phylogenetic Analysis of the Genus Blastobacter with a View to its Future Reclassification.
  • Hirsch1985 - Blastobacter aggregatus sp. nov., Blastobacter capsulatus sp. nov., and Blastobacter denitrificans sp. nov., New Budding Bacteria from Freshwater Habitats.
  • Trotha2002 - Disseminated Infection Due to Blastobacter denitrificans following Routine Appendectomy in an Adolescent.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BRADYRHIZOBIUM DENITRIFICANS
  • Bik2006 - Molecular analysis of the bacterial microbiota in the human stomach.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021