Brevibacillus borstelensis

(aka Bacillus borstelensis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brevibacillus borstelensis, (aka Bacillus borstelensis), is a Gram-positive, spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 50-52%. Brevibacillus borstelensis is probably a rare gut coloniser. (Shida1996; Logan2011bBergey; Shida1995)



  • This organism has been recovered from human faeces, soil and clinical sources (blood - CCUG). The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Could be a possible pathogen in humans, but unknown at this stage. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Shida1996); (Logan2011bBergey); (Shida1995);
    Character Response
  • Substrates hydrolysed or digested:
  • casein; DNA; gelatin; tyrosine;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 2.0% salt;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.6; Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 15℃; grows at 20℃; grows at 50℃; Grows optimally at 30℃.
  • ±
  • Strain-dependent acid from carbs:
  • glycerol;
  • ⥅
  • Substrates assimilated or utilised:
  • ethanolamine; fructose; ribose; alanine; D-alanine; γ-aminobutyrate; proline; serine; DL-glycerate; 3-hydroxybutyrate;
  • ±
  • Strain-dependent substrate utilisation:
  • glycerol; tyrosine; 2-ketoglutarate;
  • Active enzymes:
  • catalase;
    • SPECIAL FEATURES (Logan2011bBergey); (Shida1995);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • VP test:
  • activity is variable
  • ✗
  • ONPG test:
  • not active
  • Lysozyme:
  • inhibited (0.001%)
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • ±
  • Pigments:
  • brown-red

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Paenibacillaceae Genus:  Brevibacillus Alt. name:  Bacillus borstelensis Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod Pigment:  brown-red
    Health:  Unknown
    Source:  human faeces, soil and clinical sources (blood - CCUG)
    DNA G+C(%):  50-52
    Opt. T:  30℃
    Low T(℃):  15(neg)
    Lower T(℃):  20(+)
    High T(℃):  50(+)
    NaCl 0.5-2%:  2.0(neg)
    Opt. pH:  7.0
    pH 4.2-5.9:  5.6(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  + Starch:  neg Casein:  + DNA:  + Tyrosine:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  w Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg Rhamnose:  neg Ribose:  w(neg) Sorbose:  neg D-Tagatose:  w(neg) Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  d(w) Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  w(neg) Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  + Galactose:  neg Glucose:  neg Rhamnose:  neg Ribose:  + Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Isomaltose:  neg Lactose:  neg Maltose:  neg Maltotriose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Adonitol:  neg L-Arabitol:  neg Ethanolamine:  + Gluconate:  neg Glycerol:  d Inositol:  neg Mannitol:  neg Me-α-D-Glc:  neg Sorbitol:  neg Ala:  + D-Ala:  + 4-Aminobutyrate:  + Asp:  neg Pro:  + Ser:  + Trp:  neg Tyr:  d Acetate:  neg Citrate:  neg Fumarate:  neg Glutarate:  neg DL-Glycerate:  + 3-Hydroxybutyrate:  + 2-Ketogluconate:  neg 5-Ketogluconate:  neg L-Malate:  neg Mucate:  neg Phenylacetate:  neg Propionate:  neg Quinate:  neg Saccharate:  neg Succinate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg β-Galactosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg Lecithinase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  brown-red

    References

    SPECIFIC REFERENCES FOR BREVIBACILLUS BORSTELENSIS
  • Shida1996 - Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.
  • Logan2011bBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Paenibacillaceae, Genus IV. Brevibacillus
  • Shida1995 - Proposal of Bacillus reuszeri sp. nov., Bacillus formosus sp. nov., nom. rev., and Bacillus borstelensis sp. nov., nom. rev.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BREVIBACILLUS BORSTELENSIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR BREVIBACILLUS BORSTELENSIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021