Brevibacillus centrosporus

(aka Bacillus centrosporus)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brevibacillus centrosporus, (aka Bacillus centrosporus), is a Gram-positive, spore-forming, strictly aerobic, motile, rod-shaped bacterium. The DNA G+C content is 49-51%. Brevibacillus centrosporus is likely to be transient and not a long-term gut coloniser. (Shida1996; Logan2011bBergey; Nakamura1993)



  • This organism has been recovered from human faeces (child - Logan2011bBergey), clinical sources (respiratory lavage, blood), spinach and seagrass. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). Can cause opportunistic infections, particularly in immunocompromised people. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Borderline cold mesophile, with optimum growth temp of 28C. Unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Shida1996); (Logan2011bBergey); (Nakamura1993);
    Character Response
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 3.0% salt;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.6; Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 10℃; grows at 40℃; doesn't grow at 50℃; Grows optimally at 28℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; rhamnose; ribose; cellubiose; maltose; sucrose; trehalose; mannitol;
  • ⥅
  • Substrates assimilated or utilised:
  • glucose; mannitol; rhamnose; 2-ketoglutarate; phenylacetate; succinate;
  • ±
  • Strain-dependent substrate utilisation:
  • ribose; tyrosine; citrate; 3-hydroxybutyrate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; esterase C4; esterase lipase C8;
    • SPECIAL FEATURES (Logan2011bBergey); (Nakamura1993);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S; indole;
  • ✗
  • VP test:
  • not active
  • ✗
  • ONPG test:
  • not active
  • Lysozyme:
  • inhibited (0.001%)
  • Nitrate:
  • reduction is variable
  • ±
  • NO3➔NO2:
  • strain dependent
  • ✗
  • Pigments:
  • not produced

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Paenibacillaceae Genus:  Brevibacillus Alt. name:  Bacillus centrosporus Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod Pigment:  neg
    Health:  Unknown
    Source:  human faeces (child - Logan2011bBergey), clinical sources (respiratory lavage, blood), spinach and seagrass
    DNA G+C(%):  49-51
    Opt. T:  28℃
    Low T(℃):  10(+)
    Mid T(℃):  40(+)
    High T(℃):  50(neg)
    NaCl 3-5%:  3.0(neg)
    Opt. pH:  7.0
    pH 4.2-5.9:  5.6(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  vr Starch:  neg Casein:  neg Alginate:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + Mannose:  neg Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  + Gentiobiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Sucrose:  + Trehalose:  + Turanose:  neg Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  w(neg) Inositol:  neg Mannitol:  + Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  w(neg) Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  neg Galactose:  neg Glucose:  + Rhamnose:  + Ribose:  d Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Isomaltose:  neg Lactose:  neg Maltose:  neg Maltotriose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Adonitol:  neg L-Arabitol:  neg Ethanolamine:  neg Glycerol:  neg Inositol:  neg Mannitol:  + Me-α-D-Glc:  neg Sorbitol:  neg Ala:  neg D-Ala:  neg 4-Aminobutyrate:  neg Pro:  neg Ser:  neg Trp:  neg Tyr:  d Citrate:  d DL-Glycerate:  neg 3-Hydroxybutyrate:  d 2-Ketogluconate:  neg 5-Ketogluconate:  neg L-Malate:  neg Mucate:  neg Phenylacetate:  + Propionate:  neg Quinate:  neg Saccharate:  neg Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg LeuAA:  neg PyrrolidAA:  neg AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  + Lecithinase:  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  neg

    References

    SPECIFIC REFERENCES FOR BREVIBACILLUS CENTROSPORUS
  • Shida1996 - Proposal for Two New Genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.
  • Logan2011bBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Paenibacillaceae, Genus IV. Brevibacillus
  • Nakamura1993 - DNA Relatedness of Bacillus brevis Migula 1900 Strains and Proposal of Bacillus agri sp. nov., nom. rev., and Bacillus centrosporus sp. nov., nom. rev.
  • ...............................
    • GENERAL REFERENCES FOR BREVIBACILLUS CENTROSPORUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 22, 2021