Brevundimonas diminuta

(aka Pseudomonas diminuta)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brevundimonas diminuta, (aka Pseudomonas diminuta), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 6 gut microbiome compilation studies or metastudies. The DNA G+C content is 66-68%. Brevundimonas diminuta is probably a rare gut coloniser. (Segers1994)



  • This organism has been recovered from human faeces and clinical sources (blood, wound, urine - CCUG). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Segers1994);
    Character Response
  • 🧂
  • Salt tolerance:
  • Grows optimally with 0.5-2.0% salt.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 4℃; Grows optimally at 30-37℃.
  • ⥅
  • Substrates assimilated or utilised:
  • aspartate; glutamate; histidine; isoleucine; leucine; proline; serine; threonine; acetate; butyrate; 3-hydroxybutyrate; isovalerate; pyruvate; glutamine; norvaline;
  • ±
  • Strain-dependent substrate utilisation:
  • alanine; arginine; norleucine; 2-ketogluconate; 2-ketoglutarate; L-malate; succinate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; chymotrypsin; esterase lipase C8; Leu arylamidase; lipase; oxidase; phosphoamidase; trypsin;
    • SPECIAL FEATURES (Segers1994);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • cream
    • RESPONSE TO ANTIBIOTICS (Han2005);
    Class Active Resistant
  • Penicillins:
  • imipenem; penicillin G; ticarcillin-clavulanic acid;
  • ampicillin;
  • Cephalosporins:
  • cefepime; ceftazidime;
  • Quinolines:
  • gatifloxacin;
  • ciprofloxacin;
  • Aminoglycosides:
  • amikacin; streptomycin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;
  • NOTES

    Requires both biotin and cyanocobalamin for growth to occur.

  • Walker, A. W., Sanderson, J. D., Churcher, C., Parkes, G. C., Hudspith, B. N., Rayment, N., Brostoff, J., Parkhill, J., Dougan, G., & Petrovska, L. (2011). High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease. BMC Microbiology, 11, 7.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Caulobacterales Family:  Caulobacteraceae Genus:  Brevundimonas Alt. name:  Pseudomonas diminuta Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  cream
    Health:  Unknown
    Source:  human faeces and clinical sources (blood, wound, urine - CCUG)
    DNA G+C(%):  66-68
    Opt. T:  30-37℃
    Low T(℃):  4(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  neg Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Glucose:  neg Rhamnose:  neg Ribose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Sucrose:  neg Trehalose:  neg Glycogen:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg 5-Ketogluconate:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg D-Lyxose:  neg Mannose:  neg Rhamnose:  neg Ribose:  neg D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg N_Acetyl_glucosamine:  neg Adonitol:  neg Amygdalin:  neg D-Arabitol:  neg L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  neg Ethanolamine:  neg Gluconate:  neg Glycerol:  neg Glycogen:  neg Inositol:  neg Inulin:  neg Mannitol:  neg Me-α-D-Glc:  neg Methanol:  neg Salicin:  neg Sorbitol:  neg Starch:  neg Xylitol:  neg Ala:  d(neg) D-Ala:  vr 4-Aminobutyrate:  neg 5-Aminovalerate:  neg Arg:  d(neg) Asp:  + Betaine:  neg Cys:  neg Glu:  + Gly:  neg Histamine:  neg His:  + Ile:  d(+) DL-Kynurenine:  neg Leu:  + Lys:  neg Met:  neg Norleucine:  d(neg) Orn:  neg Phe:  neg Pro:  + Sarcosine:  neg Ser:  + Thr:  d(+) Trigonelline:  neg Tryptamine:  neg Trp:  neg Tyr:  vr Acetate:  + Aconitate:  neg Adipate:  neg Azelate:  neg Benzoate:  neg Butyrate:  + Caprate:  neg Caproate:  neg Caprylate:  neg Citraconate:  neg Citrate:  neg Fumarate:  vr Glutarate:  neg Glycerate:  neg DL-Glycerate:  neg Heptanoate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Isobutyrate:  neg Isovalerate:  d(+) Itaconate:  neg 2-Ketogluconate:  d(neg) 5-Ketogluconate:  neg Lactate:  neg DL-Lactate:  neg Levulinate:  neg Malate:  neg L-Malate:  d(neg) Maleate:  neg Malonate:  neg L-Mandelate:  neg Mesaconate:  neg Mucate:  neg Phenol:  neg Phenylacetate:  neg Pimelate:  neg Propionate:  neg Pyruvate:  + Quinate:  neg Saccharate:  neg Sebacate:  neg Suberate:  neg Succinate:  d(neg) D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  d(+) LysDC:  neg OrnDC:  neg Trypsin:  + LeuAA:  + PyrrolidAA:  neg ValAA:  neg AlkalineP:  + AcidP:  + Esterase(C4):  vr EstLip(C8):  + Lecithinase:  neg Lipase:  + Lipase(C14):  neg Phosphoamidase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg Pigment:  cream
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  R(32)
    penicillin_G:  Sens
    tica-clav:  S(6)
    imipenem:  S(2)
    cefepime:  R(>32)
    ceftazidime:  R(128)
    amikacin:  S(6)
    streptomycin:  Sens
    ciprofloxacin:  R(8)
    gatifloxacin:  S(1)
    levofloxacin:  I(3)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    SXT:  S(0.05)

    References

    SPECIFIC REFERENCES FOR BREVUNDIMONAS DIMINUTA
  • Han2005 - Brevundimonas diminuta infections and its resistance to fluoroquinolones.
  • Segers1994 - Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Busing, Doll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., Respectively.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BREVUNDIMONAS DIMINUTA
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • PerezBrocal2015 - Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects Potential Interactions and Biomarkers
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • Added: April 08, 2021