Brevundimonas vesicularis

(aka Pseudomonas vesicularis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brevundimonas vesicularis, (aka Pseudomonas vesicularis), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 65-66%. Brevundimonas vesicularis is probably a rare gut coloniser. (Segers1994)



  • This organism has been recovered from clinical sources (wounds, vagina, pleural liquid - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). It is an opportunistic pathogen. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Segers1994);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • Grows optimally with 0.5-2.0% salt.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 4℃; Grows optimally at 30-37℃.
  • ⥅
  • Substrates assimilated or utilised:
  • glucose; maltose; aspartate; glutamate; histidine; isoleucine; leucine; proline; serine; threonine; acetate; butyrate; 3-hydroxybutyrate; isovalerate; pyruvate; glutamine;
  • ±
  • Strain-dependent substrate utilisation:
  • alanine; arginine; norleucine; 2-ketogluconate; L-malate; succinate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; chymotrypsin; esterase lipase C8; Leu arylamidase; lipase; phosphoamidase; trypsin;
    • SPECIAL FEATURES (Segers1994);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • cream
    • RESPONSE TO ANTIBIOTICS (Stabler2018);
    Class Active Resistant
  • Penicillins:
  • ertapenem; meropenem; piperacillin; piperacillin-tazobactam;
  • amoxicillin-clavulanic acid; ticarcillin-clavulanic acid;
  • Cephalosporins:
  • cefepime; cefotetan; ceftazidime;
  • Aminoglycosides:
  • gentamicin; tobramycin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;

  • Ley, R. E., Turnbaugh, P. J., Klein, S., & Gordon, J. I. (2006). Microbial ecology: human gut microbes associated with obesity. Nature, 444(7122), 1022–1023.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Caulobacterales Family:  Caulobacteraceae Genus:  Brevundimonas Alt. name:  Pseudomonas vesicularis Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  cream
    Health:  Unknown
    Source:  clinical sources (wounds, vagina, pleural liquid - CCUG) and human faeces
    DNA G+C(%):  65-66
    Opt. T:  30-37℃
    Low T(℃):  4(neg)
    		Aesculin:  + Urea:  neg Gelatin:  neg Starch:  neg Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Glucose:  neg Rhamnose:  neg Ribose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Sucrose:  neg Trehalose:  neg Glycogen:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg 5-Ketogluconate:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + D-Lyxose:  neg Mannose:  neg Rhamnose:  neg Ribose:  neg D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg N_Acetyl_glucosamine:  neg Adonitol:  neg Amygdalin:  neg D-Arabitol:  neg L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  neg Ethanolamine:  neg Gluconate:  neg Glycerol:  neg Glycogen:  neg Inositol:  neg Inulin:  neg Mannitol:  neg Me-α-D-Glc:  neg Methanol:  neg Salicin:  neg Sorbitol:  neg Starch:  neg Xylitol:  neg Ala:  d(neg) D-Ala:  vr 4-Aminobutyrate:  neg 5-Aminovalerate:  neg Arg:  d(neg) Asp:  + Betaine:  neg Cys:  neg Glu:  + Gly:  neg Histamine:  neg His:  + Ile:  d(+) DL-Kynurenine:  neg Leu:  + Lys:  neg Met:  neg Norleucine:  d(neg) Orn:  neg Phe:  neg Pro:  + Sarcosine:  neg Ser:  + Thr:  d(+) Trigonelline:  neg Tryptamine:  neg Trp:  neg Tyr:  vr Val:  neg Acetate:  + Aconitate:  neg Adipate:  neg Azelate:  neg Benzoate:  neg Butyrate:  + Caprate:  neg Caproate:  neg Caprylate:  neg Citraconate:  neg Citrate:  neg Fumarate:  vr Glutarate:  neg Glycerate:  neg DL-Glycerate:  neg Heptanoate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Isobutyrate:  neg Isovalerate:  d(+) Itaconate:  neg 2-Ketogluconate:  d(neg) 5-Ketogluconate:  neg Lactate:  neg DL-Lactate:  neg Levulinate:  neg Malate:  neg L-Malate:  d(neg) Maleate:  neg Malonate:  neg L-Mandelate:  neg Mesaconate:  neg Mucate:  neg Phenol:  neg Phenylacetate:  neg Pimelate:  neg Propionate:  neg Pyruvate:  + Quinate:  neg Saccharate:  neg Sebacate:  neg Suberate:  neg Succinate:  d(neg) D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  d(+) LysDC:  neg OrnDC:  neg Trypsin:  + LeuAA:  + PyrrolidAA:  neg ValAA:  neg AlkalineP:  + AcidP:  + Esterase(C4):  vr EstLip(C8):  + Lecithinase:  neg Lipase:  + Lipase(C14):  neg Phosphoamidase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg Pigment:  cream
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  R(MIC50): 8, MIC90: 32, RNG: (0.12->32)
    piperacillin:  Sens
    piper-taz:  Sens
    tica-clav:  R(MIC50): 16, MIC90: >64, RNG: (4->64)
    ertapenem:  S(MIC50): 0.03, MIC90: 4, RNG: (≤0.008->16)
    imipenem:  Var(MIC50): 0.5, MIC90: >64, RNG: (0.25->64)
    meropenem:  Sens
    cefepime:  R(MIC50): 8, MIC90: 32, RNG: (0.25-32)
    cefotetan:  R(MIC50): 32, MIC90: >64, RNG: (8->64)
    ceftazidime:  R(MIC50): 32, MIC90: >64, RNG: (0.5->64)
    gentamicin:  Sens
    tobramycin:  Sens
    ciprofloxacin:  Var(MIC50): 4, MIC90: 16, RNG: (0.25-16)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    SXT:  Sens

    References

    SPECIFIC REFERENCES FOR BREVUNDIMONAS VESICULARIS
  • Segers1994 - Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Busing, Doll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., Respectively.
  • Stabler2018 - Brevundimonas vesicularis Causing Bilateral Pneumosepsis in an Immunocompetent Adult: A Case Report and Literature Review.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BREVUNDIMONAS VESICULARIS
  • Bik2006 - Molecular analysis of the bacterial microbiota in the human stomach.
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021