Brucella anthropi

(aka Ochrobactrum anthropi)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brucella anthropi, (aka Ochrobactrum anthropi), is a Gram-negative, non-spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 56-59%. Brucella anthropi is likely to be transient and not a long-term gut coloniser. (Holmes1988; Velasco1998; Holmes2005Bergey)



  • This organism has been recovered from clinical sources (infection, blood, cerebrospinal fluid - CCUG) and human faeces (CCUG - as Ochrobactrum anthropi). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread (notes: human and animal pathogen). It is an opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe; unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Holmes1988); (Velasco1998); (Holmes2005Bergey);
    Character Response
  • 🌡
  • Temperature tolerance:
  • grows at 18℃; grows at 37℃; Grows optimally at 20-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • arabinose; fructose; glucose; rhamnose; xylose; cellubiose; maltose; sucrose; trehalose; adonitol; glycerol; inositol; mannitol; sorbitol;
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; arabinose; L-arabinose; d_arabitol; cellubiose; dulcitol; erythritol; fucose; D-fucose; galactose; gluconate; gentiobiose; glycerol; inositol; D-lyxose; maltose; mannitol; rhamnose; ribose; sorbitol; sucrose; D-tagatose; trehalose; D-turanose; xylose; D-alanine; β-alanine; δ-aminovalerate; arginine; aspartate; betaine; L-citrulline; glycine; isoleucine; leucine; lysine; norleucine; ornithine; sarcosine; serine; valine; butyrate; caprate; citrate; fumarate; glutarate; glycolate; 3-hydroxybutyrate; 4-hydroxybenzoate; isobutyrate; 2-ketogluconate; 5-ketogluconate; 2-ketoglutarate; lactate; DL-lactate; L-malate; propionate; pyruvate; succinate; valerate; norvaline; D-malate;
  • ±
  • Strain-dependent substrate utilisation:
  • arbutin; methyl-α-glucoside; salicin; xylitol; 2-aminobutyrate; cysteine; caproate; isovalerate; maleate; nonanoate; 2-aminobenzoate;
  • Active enzymes:
  • Ala arylamidase; arabinosidase; catalase; β-glucosidase; Gly arylamidase; oxidase; pyrrolidine arylamidase; urease;
  • ±
  • Strain-dependent active enzymes:
  • His arylamidase; Leu arylamidase; Phe arylamidase;
    • SPECIAL FEATURES (Holmes1988); (Velasco1998); (Holmes2005Bergey);
    Character Response
  • ✓
  • Metabolites produced:
  • H₂S (variable);
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • ONPG test:
  • not active
  • ±
  • KCN growth:
  • strain-dependent
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • Nitrite:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Holmes1988); (Holmes2005Bergey);
    Class Active Resistant
  • Penicillins:
  • carbenicillin; imipenem;
  • amoxicillin-clavulanic acid; ticarcillin-clavulanic acid;
  • Cephalosporins:
  • cefepime; cefotetan; ceftazidime;
  • Quinolines:
  • ciprofloxacin; nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; neomycin; streptomycin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Brucellaceae Genus:  Brucella Alt. name:  Ochrobactrum anthropi Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  clinical sources (infection, blood, cerebrospinal fluid - CCUG) and human faeces (CCUG - as Ochrobactrum anthropi)
    DNA G+C(%):  56-59
    Opt. T:  20-37℃
    Lower T(℃):  18(+)
    Mid T(℃):  37(+)
    		Aesculin:  neg Gelatin:  neg DNA:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  + Fructose:  + Glucose:  + Rhamnose:  + Xylose:  + Cellubiose:  + Lactose:  neg Maltose:  + Sucrose:  + Trehalose:  d(+) Adonitol:  + Glycerol:  + Inositol:  + Mannitol:  + Sorbitol:  d(+) Ethanol:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  + Fucose:  + D-Fucose:  + Galactose:  + Glucose:  neg D-Lyxose:  + Mannose:  vr Rhamnose:  + Ribose:  + D-Tagatose:  + Xylose:  + Cellubiose:  + Gentiobiose:  + Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  + D-Turanose:  + Trehalose:  + N_Acetyl_glucosamine:  + Adonitol:  vr Amygdalin:  neg D-Arabitol:  + L-Arabitol:  neg Arbutin:  d Dulcitol:  + Erythritol:  + Aesculin:  neg Gluconate:  + Glycerol:  + Inositol:  + Mannitol:  + Me-α-D-Glc:  d Salicin:  d Sorbitol:  + Starch:  neg Xylitol:  d D-Ala:  + β-Ala:  + 5-Aminovalerate:  + Arg:  + Asp:  + Betaine:  + L-Citrulline:  + Cys:  d Gly:  + Ile:  + Leu:  + Lys:  + Met:  neg Norleucine:  + Orn:  + Phe:  neg Sarcosine:  + Ser:  + Trigonelline:  neg Trp:  neg Tyr:  neg Val:  + Aconitate:  neg Adipate:  neg Azelate:  neg Benzoate:  neg Butyrate:  + Caprate:  + Caproate:  d(neg) Citraconate:  neg Citrate:  + Fumarate:  + Glutarate:  + Glycolate:  + 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  d(+) Isobutyrate:  + Isovalerate:  d(neg) 2-Ketogluconate:  + 5-Ketogluconate:  d(+) Lactate:  + DL-Lactate:  + L-Malate:  + Maleate:  d(neg) Malonate:  neg L-Mandelate:  neg Nonanoate:  d(neg) Phenylacetate:  neg Propionate:  + Pyruvate:  + Sebacate:  neg Suberate:  neg Succinate:  + D-Tartrate:  neg Valerate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  + α-Arab:  + Ac-β-glcamnd:  neg β-Galactosidase:  neg β-Glucosidase:  + β-Glucuronidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg AlanineAA:  + GlyAA:  + HisAA:  d LeuAA:  d ProAA:  neg PyrrolidAA:  + PheAA:  d(neg) TyrAA:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  vr Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  R(MIC50): >64, MIC90: >64, RNG: (32->64)
    carbenicil:  Sens
    tica-clav:  R(MIC50): >64, MIC90: >64, RNG: (64->64)
    imipenem:  S(MIC50): 1, MIC90: 2, RNG: (1-2)
    cefepime:  R(MIC50): 16, MIC90: 64, RNG: (8-64)
    cefotetan:  R(MIC50): 64, MIC90: >64, RNG: (16->64)
    ceftazidime:  R(MIC50): >64, MIC90: >64, RNG: (64->64)
    gentamicin:  Sens
    neomycin:  Sens
    streptomycin:  Sens
    ciprofloxacin:  S(MIC50): 0.5, MIC90: 0.5, RNG: (≤0.06-0.5)
    nalidixic-acid:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    SXT:  Sens

    References

    SPECIFIC REFERENCES FOR BRUCELLA ANTHROPI
  • Holmes1988 - Ochrobactrum anthropi gen. nov., sp. nov. from Human Clinical Specimens and Previously Known as Group Vd.
  • Velasco1998 - Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.
  • Holmes2005Bergey - Bergey's manual of systematic bacteriology. Vol. 2, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria Part C. Family Brucellaceae, Genus III. Ochrobactrum
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BRUCELLA ANTHROPI
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR BRUCELLA ANTHROPI
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: July 12, 2021