Brucella cytisi

(aka Ochrobactrum cytisi)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brucella cytisi, (aka Ochrobactrum cytisi), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 56.4%. Brucella cytisi is probably a rare gut coloniser. (ZurdoPineiro2007)



  • This organism has been recovered from root nodules. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (ZurdoPineiro2007);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🌡
  • Temperature tolerance:
  • grows at 25-30℃;
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; adonitol; arabinose; L-arabinose; d_arabitol; arbutin; erythritol; fucose; D-fucose; glucose; maltose; mannitol; mannose; rhamnose; ribose; sucrose; D-tagatose; D-turanose; citrate; 2-ketogluconate; 5-ketogluconate;
  • Active enzymes:
  • catalase; chymotrypsin; cystine arylamidase; esterase C4; esterase lipase C8; α-glucosidase; Leu arylamidase; lipase C14; oxidase; phosphoamidase; trypsin; Val arylamidase;
  • ±
  • Strain-dependent active enzymes:
  • β-galactosidase;
    • SPECIAL FEATURES (ZurdoPineiro2007);
    Character Response
  • ✓
  • Metabolites produced:
  • indole;
  • ✓
  • VP test:
  • active
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (ZurdoPineiro2007);
    Class Active Resistant
  • Penicillins:
  • ampicillin; cloxacillin; penicillin;
  • Cephalosporins:
  • cefuroxime;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • oxytetracycline;
  • Quinolines:
  • ciprofloxacin;
  • Aminoglycosides:
  • neomycin;
  • Heterocycles:
  • chloramphenicol;
  • Miscellaneous antibiotics:
  • polymyxin B;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Brucellaceae Genus:  Brucella Alt. name:  Ochrobactrum cytisi Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  root nodules
    DNA G+C(%):  56.4
    Lower T(℃):  25-30(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  + Fructose:  w Fucose:  + D-Fucose:  + Galactose:  w Glucose:  + Mannose:  + Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  + Xylose:  w Cellubiose:  w Gentiobiose:  neg Lactose:  w Maltose:  + Melezitose:  neg Raffinose:  neg Sucrose:  + D-Turanose:  + Trehalose:  w N_Acetyl_glucosamine:  + Adonitol:  + Amygdalin:  neg D-Arabitol:  + L-Arabitol:  neg Arbutin:  + Dulcitol:  w Erythritol:  + Gluconate:  neg Glycerol:  w Glycogen:  neg Inositol:  w Inulin:  neg Mannitol:  + Me-α-D-Glc:  w Salicin:  neg Sorbitol:  neg Starch:  neg Xylitol:  neg Adipate:  neg Caprate:  neg Citrate:  + 2-Ketogluconate:  + 5-Ketogluconate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  d α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  + LysDC:  neg OrnDC:  neg Trypsin:  + CystineAA:  + LeuAA:  + ValAA:  + Esterase(C4):  + EstLip(C8):  + Lipase(C14):  + Phosphoamidase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  R(2; disc)
    cloxacillin:  R(1; disc)
    penicillin:  R(10U)
    cefuroxime:  R(30; disc)
    gentamicin:  Var(MIC50): 10; disc), MIC90: Var(10; disc
    neomycin:  R(5; disc)
    erythromycin:  R(2; disc)
    ciprofloxacin:  S(5; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    oxytetracycline:  R(30; disc)
    chloramphenicol:  Res
    polymyxin_B:  R(300U)

    References

    SPECIFIC REFERENCES FOR BRUCELLA CYTISI
  • ZurdoPineiro2007 - Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BRUCELLA CYTISI
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • ...............................
    • Added: February 09, 2022