Brucella melitensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brucella melitensis is a Gram-negative, non-spore-forming, aerobic, non-motile, oval-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 58%. Brucella melitensis is unlikely to be a gut coloniser. (Meyer1920; Corbel2005Bergey)



  • This organism has been recovered from humans and animals. The risk classification (www.baua.de) for this organism is 3, i.e., high risk of individual infection, and some risk of spread (notes: zoonotic pathogen). This is a dangerous pathogen. Unlikely to be a commensal. Robust growth can have negative consequences for gut health.
    • GENERAL CHARACTERISTICS (Meyer1920); (Corbel2005Bergey);
    Character Response
  • ⥅
  • Substrates assimilated or utilised:
  • erythritol; glucose; alanine; glutamate; glutamine;
  • Active enzymes:
  • catalase;
    • SPECIAL FEATURES (Corbel2005Bergey);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • VP test:
  • not active
  • ✓
  • Nitrate:
  • reduced
  • ±
  • Pigments:
  • golden yellow
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Tetracyclines:
  • doxycycline;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Brucellaceae Genus:  Brucella Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Oval-shaped Pigment:  golden yellow
    Health:   Negative
    Source:  humans and animals
    DNA G+C(%):  58
    		Gelatin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Galactose:  neg Glucose:  + Ribose:  neg Xylose:  neg Erythritol:  + Ala:  + Arg:  neg L-Citrulline:  neg Glu:  + Lys:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  golden yellow
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    azithromycin:  Var(MIC50): <0.125->256), MIC90: Var(<0.125->256
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  SensRNG: (0.12-8)

    References

    SPECIFIC REFERENCES FOR BRUCELLA MELITENSIS
  • Meyer1920 - A Comparison of the Morphologic, Cultural and Biochemical Characteristics of B. Abortus and B. Melitensis: Studies on the Genus Brucella Nov. Gen. I
  • Ficht1996 - Species-specific sequences at the omp2 locus of Brucella type strains.
  • Corbel2005Bergey - Bergey's manual of systematic bacteriology. Vol. 2, The The Alpha-, Beta-, Delta-, and Epsilonproteobacteria Part C. Family Brucellaceae, Genus I. Brucella
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BRUCELLA MELITENSIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • ...............................
    • Added: January 14, 2022