Brucella tritici

(aka Ochrobactrum tritici)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Brucella tritici, (aka Ochrobactrum tritici), is a Gram-negative, non-spore-forming, strictly aerobic, motile, bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 59%. Brucella tritici is likely to be transient and not a long-term gut coloniser. (Lebuhn2000)



  • This organism has been recovered from wheat rhizosphere. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe; unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Lebuhn2000);
    Character Response
  • Substrates hydrolysed or digested:
  • urea;
  • pH
  • Acidity tolerance:
  • tolerates pH 3.0; tolerates pH 9; Grows optimally at pH 6-7.
  • 🌡
  • Temperature tolerance:
  • grows at 4℃; grows at 40℃; Grows optimally at 30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • arabinose; fructose; xylose;
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; adonitol; L-arabinose; d_arabitol; dextrin; erythritol; fructose; fucose; galactose; gluconate; glucose; glycogen; inositol; maltose; rhamnose; sorbitol; sucrose; trehalose; D-turanose; alanine; D-alanine; γ-aminobutyrate; aspartate; glutamate; glycine; histidine; leucine; ornithine; proline; serine; threonine; acetate; aconitate; formate; 3-hydroxybutyrate; DL-lactate; malate; propionate; sebacate; succinate; psicose; glutamine; L-asparagine; alaninamide; L-hydroxyproline; α-ketovalerate; α-ketobutyrate; D-glucuronate; methyl succinate; methyl pyruvate; inosine;
  • ±
  • Strain-dependent substrate utilisation:
  • mannose; citrate;
  • Active enzymes:
  • catalase; oxidase; urease;
    • SPECIAL FEATURES (Lebuhn2000);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • ONPG test:
  • not active
  • ✓
  • NO3➔NO2:
  • reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS (Lebuhn2000);
    Class Active Resistant
  • Miscellaneous antibiotics:
  • colistin; polymyxin B;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Brucellaceae Genus:  Brucella Alt. name:  Ochrobactrum tritici Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Pigment:  neg
    Health:  Unknown
    Source:  wheat rhizosphere
    DNA G+C(%):  59
    Opt. T:  30℃
    Low T(℃):  4(+)
    Mid T(℃):  40(+)
    Opt. pH:  6-7
    pH 4.2-5.9:  3.0(+)
    pH >8:  9(+)
    		Aesculin:  neg Urea:  + Gelatin:  neg Tween:  40(+) 80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  + Fructose:  + Glucose:  neg Rhamnose:  neg Xylose:  + Sucrose:  neg Amygdalin:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Ethanol:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  + Fructose:  + Fucose:  + Galactose:  + Glucose:  + Mannose:  d Rhamnose:  + Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  + Melibiose:  neg Raffinose:  neg Sucrose:  + D-Turanose:  + Trehalose:  + N_Acetyl_glucosamine:  d(+) Adonitol:  + D-Arabitol:  + Dextrin:  + Erythritol:  + Ethanolamine:  neg Gluconate:  + Glycerol:  neg Glycogen:  + Inositol:  + Mannitol:  neg Sorbitol:  + Xylitol:  neg Ala:  + D-Ala:  + 4-Aminobutyrate:  + Asp:  + Glu:  + Gly:  + His:  + Leu:  + Orn:  + Phe:  neg Pro:  + Ser:  + Thr:  + Acetate:  + Aconitate:  cis(+) Caprate:  w Citrate:  d Formate:  + 3-Hydroxybutyrate:  + Itaconate:  neg DL-Lactate:  + Malate:  + Malonate:  neg Phenylacetate:  neg Propionate:  + Quinate:  neg Sebacate:  + Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  + β-Galactosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg TrpAA:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    chloramphenicol:  Var
    colistin:  Sens
    polymyxin_B:  Sens

    References

    SPECIFIC REFERENCES FOR BRUCELLA TRITICI
  • Lebuhn2000 - Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR BRUCELLA TRITICI
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • ...............................
    • Added: January 14, 2022