Caldibacillus thermoamylovorans

(aka Bacillus thermoamylovorans)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Caldibacillus thermoamylovorans, (aka Bacillus thermoamylovorans), is a Gram-positive, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 5 gut microbiome compilation studies or metastudies. The DNA G+C content is 38.8%. Caldibacillus thermoamylovorans is likely to be transient and not a long-term gut coloniser. (Logan2011Bergey; Combetblanc1995)



  • This organism has been recovered from human faeces and palm wine. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal. Robust growth can have positive consequences for gut health.
    • QUIRKS
  • High optimum growth temperature so unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Logan2011Bergey); (Combetblanc1995);
    Character Response
  • Substrates hydrolysed or digested:
  • starch;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.0; tolerates pH 6.0-8.0; doesn't tolerate pH 9.0;
  • 🌡
  • Temperature tolerance:
  • grows at 50-55, 60(neg)℃; Grows optimally at 50℃.
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; fructose; galactose; glucose; mannose; rhamnose; ribose; xylose; amygdalin; glycogen; starch; cellubiose; gentiobiose; lactose; maltose; melezitose; sucrose; trehalose; arbutin; gluconate; α-methyl glucoside; N-Ac glucosamine; salicin;
  • Active enzymes:
  • catalase;
    • SPECIAL FEATURES (Logan2011Bergey); (Combetblanc1995);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Sulfate:
  • not reduced

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Caldibacillus Alt. name:  Bacillus thermoamylovorans Gram stain:  + O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:   Positive
    Source:  human faeces and palm wine
    DNA G+C(%):  38.8
    Opt. T:  50℃
    High T(℃):  50-55(+), 60(neg)
    pH 4.2-5.9:  5.0(neg)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  9.0(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  neg Starch:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  + Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  neg Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  + Gentiobiose:  + Lactose:  w(+) Maltose:  + Melezitose:  w(+) Melibiose:  neg Sucrose:  + Trehalose:  + Turanose:  neg Amygdalin:  + Glycogen:  + Inulin:  neg Starch:  + Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  + Gluconate:  + 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  + Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  + Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg β-Galactosidase:  neg ArgDH:  vr LysDC:  neg OrnDC:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg

    References

    SPECIFIC REFERENCES FOR CALDIBACILLUS THERMOAMYLOVORANS
  • Logan2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Bacillaceae Genus I. Bacillus
  • Combetblanc1995 - Bacillus thermoamylovorans sp. nov., a Moderately Thermophilic and Amylolytic Bacterium.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CALDIBACILLUS THERMOAMYLOVORANS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Hoyles2012 - Recognition of greater diversity of Bacillus species and related bacteria in human faeces.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • PerezBrocal2015 - Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects Potential Interactions and Biomarkers
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR CALDIBACILLUS THERMOAMYLOVORANS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
  • Hoyles2012 - Recognition of greater diversity of Bacillus species and related bacteria in human faeces.
    • Added: April 08, 2021