Cellulomonas denverensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Cellulomonas denverensis is a Gram-positive, non-spore-forming, aerobic, motile, short rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 68.5%. Cellulomonas denverensis is probably a rare gut coloniser. (Brown2005; Stackebrandt2012Bergey)



  • This organism has been recovered from clinical sources (blood - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). Is a rare opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Brown2005); (Stackebrandt2012Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 25℃; grows at 35℃; grows at 45℃;
  • H+
  • Acid from carbohydrates usually produced:
  • galactose; glucose; mannose; rhamnose; ribose; xylose; cellubiose; lactose; maltose; sucrose; trehalose; sorbitol; salicin;
  • ±
  • Strain-dependent acid from carbs:
  • L-arabinose; melibiose; glycerol;
  • Active enzymes:
  • acid phosphatase; N-Ac β-glucosaminidase; catalase; β-galactosidase; α-glucosidase; β-glucosidase; Leu arylamidase; pyrrolidine arylamidase;
    • SPECIAL FEATURES (Brown2005); (Stackebrandt2012Bergey);
    Character Response
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Brown2005);
    Class Active Resistant
  • Penicillins:
  • imipenem;
  • Macrolides:
  • clarithromycin;
  • Tetracyclines:
  • minocycline;
  • Quinolines:
  • ciprofloxacin;
  • Aminoglycosides:
  • amikacin;
  • Polypep/ketides:
  • rifampicin;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Micrococcales Family:  Cellulomonadaceae Genus:  Cellulomonas Gram stain:  + O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Short Rod
    Health:  Unknown
    Source:  clinical sources (blood - CCUG) and human faeces
    DNA G+C(%):  68.5
    Lower T(℃):  25(neg)
    Mid T(℃):  35(+)
    High T(℃):  45(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Casein:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  d Galactose:  + Glucose:  + Mannose:  + Rhamnose:  + Ribose:  + Xylose:  + Cellubiose:  + Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  d Sucrose:  + Trehalose:  + Glycogen:  neg Adonitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  d Inositol:  neg Mannitol:  neg Sorbitol:  + Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Raffinose:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  + Urease:  neg Ac-β-glcamnd:  + α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg α-Mannosidase:  neg LeuAA:  + PyrrolidAA:  + AcidP:  + Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  Var(MIC50): 8, MIC90: 16
    ampicillin:  Var(MIC50): 16), MIC90: Var(16
    imipenem:  S(1)
    amikacin:  R(>32)
    clarithromycin:  S(0.5)
    ciprofloxacin:  R(>8)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    minocycline:  S(0.13)
    vancomycin:  S(0.5)
    rifampicin:  S(0.25)
    SXT:  Var(MIC50): 2, MIC90: 76
    clindamycin:  SensRNG: (2-4)

    References

    SPECIFIC REFERENCES FOR CELLULOMONAS DENVERENSIS
  • Brown2005 - Phenotypic and Genetic Characterization of Clinical Isolates of CDC Coryneform Group A-3: Proposal of a New Species of Cellulomonas , Cellulomonas denverensis sp. nov.
  • Stackebrandt2012Bergey - Bergey's manual of systematic bacteriology. Vol. 5, The Actinobacteria. Part A & B. Cellulomonadaceae, Genus I. Cellulomonas
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CELLULOMONAS DENVERENSIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • Added: April 08, 2021