Citroniella saccharovorans

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Citroniella saccharovorans is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, coccus bacterium. The DNA G+C content is 29.9%. Citroniella saccharovorans is probably a rare gut coloniser. (Patel2019)



  • This organism has been recovered from human faeces (CCUG). Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Patel2019);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 0.5% salt; tolerates 6% salt; Grows optimally with 1-3% salt.
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.9; tolerates pH 9.0; Grows optimally at pH 6.5-7.3.
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 37℃; Grows optimally at 30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; maltose;
  • ⥅
  • Substrates assimilated or utilised:
  • cellubiose; dextrin; glucose; maltose; maltotriose; D-turanose; methionine; serine; threonine; pyruvate; palatinose; L-asparagine; methyl pyruvate; uridine; inosine;
  • Active enzymes:
  • Arg arylamidase; Leu arylamidase;
    • SPECIAL FEATURES (Patel2019);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate (major); indole;
  • NOTES

    A novel Gram-stain-positive, non-motile, non-spore-forming coccus-shaped obligately anaerobic bacterium was recovered from a fecal sample obtained from an individual from a traditional community located on the southern coast of Peru. The results of analysis based on 16S rRNA gene sequencing indicated the novel bacterium to be phylogenetically distinct from other genera of members of the Peptoniphilaceae family, sharing a loose affinity with the genera Ezakiella, Finegoldia, Gallicola and Parvimonas. The major cellular fatty acids of the novel isolate were determined to be C16:0, C17:1ω8c, and C18:1ω9c. The DNA G+C content was 29.9 mol%. End products of metabolism from peptone yeast glucose broth (PYG) were determined to be acetate and methyl succinate. The diagnostic diamino acid present in the cell wall was lysine. On the basis of the phenotypic, chemotaxonomic and phylogenetic results the organism is a member of a novel genus belonging to the family Peptoniphilaceae for which the name Citroniella saccharovorans gen nov. sp. nov., is proposed. The type strain is M6.X9T (DSM 29873T=CCUG 66799T).

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Peptoniphilaceae Genus:  Citroniella Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus
    Health:  Unknown
    Source:  human faeces (CCUG)
    DNA G+C(%):  29.9
    Opt. T:  30℃
    Lower T(℃):  25(+)
    Mid T(℃):  37(+)
    NaCl 0.5-2%:  0.5(+)
    NaCl >6%:  6(+)
    Opt. pH:  6.5-7.3
    pH 4.2-5.9:  5.9(neg)
    pH >8:  9.0(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  + Mannose:  neg Maltose:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Fructose:  w Fucose:  w Galactose:  w Glucose:  + Cellubiose:  + Maltose:  + Maltotriose:  + D-Turanose:  + N_Acetyl_glucosamine:  w Dextrin:  + Met:  + Ser:  + Thr:  + Pyruvate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg ArgAA:  + HisAA:  neg LeuAA:  + ProAA:  neg PheAA:  neg PyrogluAA:  neg TyrAA:  w AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  Major(+) Indole:  +

    References

    SPECIFIC REFERENCES FOR CITRONIELLA SACCHAROVORANS
  • Patel2019 - Citroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member.
  • ...............................
    • GENERAL REFERENCES FOR CITRONIELLA SACCHAROVORANS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: April 08, 2021