Cloacibacterium normanense

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Cloacibacterium normanense is a Gram-negative, non-spore-forming, facultatively anaerobic, non-motile, pleiomorph rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 31%. Cloacibacterium normanense is probably a rare gut coloniser. (Allen2006; Editorial2010aBergey)



  • This organism has been recovered from human faeces, wastewater, and human gut. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Allen2006); (Editorial2010aBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; casein; gelatin; starch;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.9; tolerates pH 8; growth at pH >8(vr) is variable; Grows optimally at pH 7.
  • 🌡
  • Temperature tolerance:
  • grows at 18℃; grows at 36℃; doesn't grow at 40℃; Grows optimally at 30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; mannose;
  • Active enzymes:
  • Ala arylamidase; Ala-Phe-Pro arylamidase; alkaline phosphatase; acid phosphatase; Arg arylamidase; catalase; chymotrypsin; DNase; esterase lipase C8; α-glucosidase; β-glucosidase; Glu-Glu arylamidase; Gly arylamidase; His arylamidase; Leu arylamidase; Leu-Gly arylamidase; oxidase; Phe arylamidase; Pro arylamidase; Ser arylamidase; Tyr arylamidase; Val arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Allen2006); (Editorial2010aBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • pyruvate (major); indole;
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • yellow
    • RESPONSE TO ANTIBIOTICS (Allen2006); (Editorial2010aBergey);
    Class Active Resistant
  • Penicillins:
  • carbenicillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline; oxytetracycline; tetracycline;
  • Quinolines:
  • ciprofloxacin;
  • Aminoglycosides:
  • gentamicin;
  • kanamycin;
  • Heterocycles:
  • chloramphenicol;

  • Frank, D. N., St Amand, A. L., Feldman, R. A., Boedeker, E. C., Harpaz, N., & Pace, N. R. (2007). Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proceedings of the National Academy of Sciences of the United States of America, 104(34), 13780–13785.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Flavobacteriia Order:  Flavobacteriales Family:  Weeksellaceae Genus:  Cloacibacterium Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Sessile Morphology:  Pleiomorph rod Pigment:  yellow
    Health:  Unknown
    Source:  human faeces, wastewater, and human gut
    DNA G+C(%):  31
    Opt. T:  30℃
    Lower T(℃):  18(+)
    Mid T(℃):  36(+)
    High T(℃):  40(neg)
    Opt. pH:  7
    pH 4.2-5.9:  5.9(neg)
    pH 6.0-8.0:  8(+)
    pH >8:  >8(vr)
    		Aesculin:  + Urea:  neg Gelatin:  + Starch:  + Casein:  + Agar:  neg Alginate:  neg Chitin:  neg DNA:  w Pectin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Glucose:  + Mannose:  + Ribose:  neg D-Tagatose:  neg Lactose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Glycogen:  neg D-Arabitol:  neg Mannitol:  neg Sorbitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Hippurate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg α-Arab:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg α-Mannosidase:  neg β-Mannosidase:  neg ArgDH:  w Chymotrypsin:  + Trypsin:  neg AlanineAA:  + AlaPheProAA:  + ArgAA:  + GluGluAA:  + GlyAA:  + HisAA:  + LeuAA:  + LeuGlyAA:  + ProAA:  + PheAA:  + PyrogluAA:  neg SerAA:  + TyrAA:  + ValAA:  + AlkalineP:  + AcidP:  + DNAse:  + Esterase(C4):  w EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Pyruvate:  Major(+) Indole:  + Pigment:  yellow
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    carbenicil:  S(100; disc)
    gentamicin:  S(10; disc)
    kanamycin:  R(30; disc)
    erythromycin:  R(15; disc)
    ciprofloxacin:  S(5; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(30; disc)
    oxytetracycline:  S(30; disc)
    tetracycline:  S(30; disc)
    chloramphenicol:  S(30; disc)

    References

    SPECIFIC REFERENCES FOR CLOACIBACTERIUM NORMANENSE
  • Allen2006 - Cloacibacterium normanense gen. nov., sp. nov., a novel bacterium in the family Flavobacteriaceae isolated from municipal wastewater.
  • Editorial2010aBergey - Bergey's manual of systematic bacteriology. Vol. 4, The Fusobacteria. Family Flavobacteriaceae, Genus XI. Cloacibacterium
  • Cassir2015 - Clostridium butyricum Strains and Dysbiosis Linked to Necrotizing Enterocolitis in Preterm Neonates
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CLOACIBACTERIUM NORMANENSE
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Cassir2015 - Clostridium butyricum Strains and Dysbiosis Linked to Necrotizing Enterocolitis in Preterm Neonates
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021