Clostridium isatidis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Clostridium isatidis is a Gram-positive, spore-forming, anaerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 27%. Clostridium isatidis is likely to be transient and not a long-term gut coloniser. (Padden1999)



  • This organism has been recovered from a woad vat. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • Indigo is reduced. High optimum growth temperature so unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Padden1999);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • pH
  • Acidity tolerance:
  • tolerates pH 5.6; tolerates pH 9.9; Grows optimally at pH 7.2.
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 55℃; Grows optimally at 49-52℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; amygdalin; starch; cellubiose; maltose; sucrose; trehalose; sorbitol; salicin;
    • SPECIAL FEATURES (Padden1999);
    Character Response
  • ✓
  • Metabolites produced:
  • formate; acetate; lactate; ethanol; COâ‚‚; Hâ‚‚;
  • ✗
  • NO3➔NO2:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Padden1999);
    Class Active Resistant
  • Penicillins:
  • penicillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Heterocycles:
  • chloramphenicol;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Clostridiaceae Genus:  Clostridium Gram stain:  + O2 Relation.:  anaerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  a woad vat
    DNA G+C(%):  27
    Opt. T:  49-52℃
    Lower T(℃):  30(+)
    High T(℃):  55(+)
    Opt. pH:  7.2
    pH 4.2-5.9:  5.6(+)
    pH >8:  9.9(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Starch:  neg Milk:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Galactose:  + Glucose:  + Mannose:  + Ribose:  neg Xylose:  neg Cellubiose:  + Lactose:  neg Maltose:  + Melezitose:  neg Sucrose:  + Trehalose:  + Amygdalin:  + Glycogen:  neg Inulin:  neg Starch:  + Mannitol:  neg Sorbitol:  + Salicin:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    penicillin:  Sens
    erythromycin:  Res
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  Sens
    chloramphenicol:  Res

    References

    SPECIFIC REFERENCES FOR CLOSTRIDIUM ISATIDIS
  • Padden1999 - An indigo-reducing moderate thermophile from a woad vat, Clostridium isatidis sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CLOSTRIDIUM ISATIDIS
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • ...............................
    • Added: March 18, 2022