Clostridium methylpentosum

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Clostridium methylpentosum is a Gram-positive, spore-forming, strictly anaerobic, non-motile, rod-shaped - curved bacterium. It has been detected in at least 11 gut microbiome compilation studies or metastudies. The DNA G+C content is 46%. Clostridium methylpentosum is a common gut coloniser. (Himelbloom1989; Rainey2011dBergey)



  • This organism has been recovered from human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal. Robust growth can have positive consequences for gut health.
    • QUIRKS
  • Seen in 83% of subjects in study by Qin2010, but at low levels. Moderate coloniser.
    • GENERAL CHARACTERISTICS (Himelbloom1989); (Rainey2011dBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; milk; pectin; starch;
  • 💧
  • Bile tolerance:
  • Resistant to 20% bile
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 25℃; doesn't grow at 50℃; Grows optimally at 45℃.
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; fucose; rhamnose;
  • ⥅
  • Substrates assimilated or utilised:
  • arabinose; fucose; rhamnose;
    • SPECIAL FEATURES (Himelbloom1989); (Rainey2011dBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate; propanol; COâ‚‚; Hâ‚‚;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Sulfate:
  • not reduced
  • NOTES

    In the human intestinal tract, dietary pectin is a major source
    of L-rhamnose. Pectinolytic bacteria that inhabit the human
    intestine produce extracellular enzymes that depolymerize
    pectin (Jensen and Canale-Parola 1986), thus releasing Lrhamnose
    and L-rhamnose-containing oligomers that may serve as fermentable substrates for non-pectinolytic bacteria
    such as C. methylpentosum.

  • Himelbloom, B. H., & Canale-Parola, E. (1989). Clostridium methylpentosum sp. nov.: a ring-shaped intestinal bacterium that ferments only methylpentoses and pentoses. Archives of Microbiology, 151(4), 287–293.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Clostridiaceae Genus:  Clostridium Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  Endospore Motility:  Sessile Morphology:  Rod - curved
    Health:   Positive
    Source:  human faeces
    DNA G+C(%):  46
    Opt. T:  45℃
    Lower T(℃):  25(neg)
    High T(℃):  50(neg)
    Bile reaction(%):  20(+)
    		Aesculin:  + Starch:  + Milk:  + Meat:  neg Pectin:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  + Fucose:  + Rhamnose:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  neg Fructose:  neg Fucose:  + D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg Rhamnose:  + Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Raffinose:  neg Sucrose:  neg N_Acetyl_glucosamine:  neg L-Arabitol:  neg Gluconate:  neg Glycerol:  neg Mannitol:  neg Pectin:  neg Starch:  neg Lactate:  neg Pyruvate:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Propionate:  + Propanol:  + CO2:  + H2:  + Indole:  neg

    References

    SPECIFIC REFERENCES FOR CLOSTRIDIUM METHYLPENTOSUM
  • Himelbloom1989 - Clostridium methylpentosum sp. nov.: a ring-shaped intestinal bacterium that ferments only methylpentoses and pentoses.
  • Rainey2011dBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Clostridiaceae, Genus I. Clostridium - Cluster IV
  • DeAngelis2013 - Fecal microbiota and metabolome of children with autism and pervasive developmental disorder not otherwise specified
  • Finegold2010 - Pyrosequencing study of fecal microflora of autistic and control children
  • Ventura2019 - Gut microbiome of treatment-naïve MS patients of different ethnicities early in disease course
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CLOSTRIDIUM METHYLPENTOSUM
  • Dubinkina2017 - Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease
  • Jie2017 - The gut microbiome in atherosclerotic cardiovascular disease
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • PerezBrocal2015 - Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects Potential Interactions and Biomarkers
  • Qin2012 - Metagenome-wide association study of gut microbiota in type 2 diabetes
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Tyakht2013 - Human gut microbiota community structures in urban and rural populations in Russia.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • Zupancic2012 - Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome.
  • ...............................
    • GENERAL REFERENCES FOR CLOSTRIDIUM METHYLPENTOSUM
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021