Clostridium neonatale

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Clostridium neonatale is a Gram-positive, spore-forming, strictly anaerobic, motile, rod-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. The DNA G+C content is 28.5%. Clostridium neonatale is probably a common, although minor, coloniser of the gut. (Bernard2018)



  • This organism has been recovered from human faeces and clinical sources (blood - CCUG). Is a rare opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Bernard2018);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; starch;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • milk;
  • ⥅
  • Substrates assimilated or utilised:
  • cellubiose; fructose; glucose; lactose; maltose; mannose; melezitose; melibiose; ribose; raffinose; salicin; sorbitol; starch; sucrose; trehalose; xylose;
    • SPECIAL FEATURES (Bernard2018);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; butyrate; lactate; succinate (trace); Hâ‚‚;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin; ampicillin-sulbactam; imipenem; meropenem; penicillin; piperacillin; piperacillin-tazobactam;
  • Cephalosporins:
  • cefotetan; cefoxitin;
  • Tetracyclines:
  • tetracycline;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Miscellaneous antibiotics:
  • clindamycin;

  • Li, M., Wang, B., Zhang, M., Rantalainen, M., Wang, S., Zhou, H., Zhang, Y., Shen, J., Pang, X., Zhang, M., Wei, H., Chen, Y., Lu, H., Zuo, J., Su, M., Qiu, Y., Jia, W., Xiao, C., Smith, L. M., … Zhao, L. (2008). Symbiotic gut microbes modulate human metabolic phenotypes. Proceedings of the National Academy of Sciences of the United States of America, 105(6), 2117–2122.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Clostridiaceae Genus:  Clostridium Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and clinical sources (blood - CCUG)
    DNA G+C(%):  28.5
    		Aesculin:  + Gelatin:  neg Starch:  + Milk:  d Meat:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg Fructose:  + Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  + Xylose:  + Cellubiose:  + Lactose:  + Maltose:  + Melezitose:  + Melibiose:  + Raffinose:  + Sucrose:  d(+) Trehalose:  + Mannitol:  neg Salicin:  + Sorbitol:  + Starch:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg Lecithinase:  neg Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Butyrate:  + Lactate:  + Succinate:  trace(+) H2:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  Sens
    ampicillin:  Sens
    amp-sulb:  Sens
    penicillin:  Sens
    piperacillin:  Sens
    piper-taz:  Sens
    imipenem:  Sens
    meropenem:  Sens
    cefotetan:  Sens
    cefoxitin:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  Sens
    chloramphenicol:  Sens
    metronidazole:  Sens
    clindamycin:  Sens

    References

    SPECIFIC REFERENCES FOR CLOSTRIDIUM NEONATALE
  • Bernard2018 - Clostridium neonatale sp. nov. linked to necrotizing enterocolitis in neonates and a clarification of species assignable to the genus Clostridium (Prazmowski 1880) emend. Lawson and Rainey 2016.
  • Cassir2015 - Clostridium butyricum Strains and Dysbiosis Linked to Necrotizing Enterocolitis in Preterm Neonates
  • Stiemsma2016 - Shifts in Lachnospira and Clostridium sp. in the 3-month stool microbiome are associated with preschool age asthma
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CLOSTRIDIUM NEONATALE
  • Aujoulat2014 - Temporal dynamics of the very premature infant gut dominant microbiota.
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Cassir2015 - Clostridium butyricum Strains and Dysbiosis Linked to Necrotizing Enterocolitis in Preterm Neonates
  • Favier2002 - Molecular monitoring of succession of bacterial communities in human neonates.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Li2019b - Disordered intestinal microbes are associated with the activity of Systemic Lupus Erythematosus
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR CLOSTRIDIUM NEONATALE
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: April 08, 2021