Comamonas denitrificans

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Comamonas denitrificans is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 60.4-60.8%. Comamonas denitrificans is probably a rare gut coloniser. (Gumaelius2001)



  • This organism has been recovered from human faeces and activated sludge. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Gumaelius2001);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 2% salt; doesn't tolerate 9% salt;
  • pH
  • Acidity tolerance:
  • Grows optimally at pH 7.5.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 4℃; grows at 20℃; grows at 37℃; Grows optimally at 30℃.
  • ⥅
  • Substrates assimilated or utilised:
  • salicin; alanine; arginine; glutamate; lysine; citrate; fumarate; DL-glycerate; glycolate; 3-hydroxybutyrate; L-malate; malonate; pyruvate; saccharate; succinate; D-tartrate; L-tartrate; glutamine; α-ketovalerate; D-glucuronate; L-lactate;
  • ±
  • Strain-dependent substrate utilisation:
  • aesculin; 2-aminobutyrate; hippurate; serine; aconitate; 3-hydroxybenzoate; gentisate;
  • Active enzymes:
  • catalase; oxidase;
    • SPECIAL FEATURES (Gumaelius2001);
    Character Response
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • Nitrite:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • Pigments:
  • variable
    • RESPONSE TO ANTIBIOTICS (Gumaelius2001);
    Class Active Resistant
  • Penicillins:
  • ampicillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • streptomycin;
  • Heterocycles:
  • chloramphenicol;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Comamonadaceae Genus:  Comamonas Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  variable
    Health:  Unknown
    Source:  human faeces and activated sludge
    DNA G+C(%):  60.4-60.8
    Opt. T:  30℃
    Low T(℃):  4(neg)
    Lower T(℃):  20(+)
    Mid T(℃):  37(+)
    NaCl 0.5-2%:  2(+)
    NaCl >6%:  9(neg)
    Opt. pH:  7.5
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg D-Lyxose:  neg Mannose:  neg Rhamnose:  neg Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg N_Acetyl_glucosamine:  neg Adonitol:  neg Amygdalin:  neg D-Arabitol:  neg L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  neg Aesculin:  d(neg) Gluconate:  neg Glycerol:  neg Glycogen:  neg Inositol:  neg Inulin:  neg Mannitol:  neg Salicin:  + Sorbitol:  neg Starch:  neg Xylitol:  neg Ala:  + Arg:  + Glu:  + Hippurate:  d His:  neg Lys:  + Orn:  neg Ser:  d Trp:  neg Aconitate:  d Adipate:  neg Caprate:  neg Citrate:  d(+) Fumarate:  + DL-Glycerate:  + Glycolate:  + 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  d 5-Ketogluconate:  neg L-Malate:  + Maleate:  neg Malonate:  + Phenylacetate:  neg Pyruvate:  + Saccharate:  + Succinate:  + D-Tartrate:  d(+) L-Tartrate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Pigment:  variable
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(10; disc)
    penicillin_G:  Var(MIC50): 10; disc), MIC90: Var(10; disc
    streptomycin:  S(30; disc)
    erythromycin:  S(15; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(30; disc)
    rifampicin:  Var(MIC50): 5; disc), MIC90: Var(5; disc
    chloramphenicol:  S(30; disc)

    References

    SPECIFIC REFERENCES FOR COMAMONAS DENITRIFICANS
  • Gumaelius2001 - Comamonas denitrificans sp. nov., an efficient denitrifying bacterium isolated from activated sludge.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR COMAMONAS DENITRIFICANS
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
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    • Added: December 15, 2021