Corynebacterium tuberculostearicum

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Corynebacterium tuberculostearicum is a Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, pleiomorph rod-shaped bacterium. It has been detected in at least 6 gut microbiome compilation studies or metastudies. The DNA G+C content is 58%. Corynebacterium tuberculostearicum is probably a rare gut coloniser. (Brown1984; Feurer2004; Bernard2012Bergey)



  • This organism has been recovered from clinical sources (blood, wound, tissue - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. A possible gut commensal.
    • QUIRKS
  • Lipophilic.
    • GENERAL CHARACTERISTICS (Brown1984); (Feurer2004); (Bernard2012Bergey);
    Character Response
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; mannose; ribose; sucrose; glycerol; 5-ketogluconate;
  • ⥅
  • Substrates assimilated or utilised:
  • glucose; glycerol; ribose; sucrose; alanine; aspartate; glutamate; proline; serine; fumarate; 2-ketogluconate; L-malate; succinate; glutamine;
  • Active enzymes:
  • Ala arylamidase; Ala-Phe-Pro arylamidase; catalase; esterase C4; Gly arylamidase; Leu arylamidase; phosphoamidase; pyrrolidine arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Brown1984); (Feurer2004);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • VP test:
  • not active
  • ±
  • Nitrate:
  • reduced by few strains
    • RESPONSE TO ANTIBIOTICS (Tyrrell2012); (Goldstein2006a);
    Class Active Resistant
  • Penicillins:
  • ampicillin; piperacillin-tazobactam;
  • Cephalosporins:
  • cephalothin;
  • ceftazidime;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • amikacin; gentamicin; kanamycin; tobramycin;
  • Heterocycles:
  • sulfadiazine;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • daptomycin; linezolid;
  • lincomycin;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Corynebacteriales Family:  Corynebacteriaceae Genus:  Corynebacterium Gram stain:  + O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Sessile Morphology:  Pleiomorph rod
    Health:  Unknown
    Source:  clinical sources (blood, wound, tissue - CCUG) and human faeces
    DNA G+C(%):  58
    		Aesculin:  neg Urea:  neg Gelatin:  neg DNA:  neg Hippurate:  vr
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Fructose:  + Galactose:  + Glucose:  vr Mannose:  + Ribose:  + D-Tagatose:  neg Xylose:  neg Lactose:  neg Maltose:  vr Melezitose:  neg Sucrose:  d(+) Trehalose:  vr Dextrin:  neg Glycogen:  neg Inulin:  neg Starch:  neg D-Arabitol:  neg Glycerol:  + Mannitol:  neg Sorbitol:  neg Gluconate:  vr 5-Ketogluconate:  + NAc-α-GA:  vr
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Glucose:  + Ribose:  + Sucrose:  + Glycerol:  + Ala:  + Asp:  + Glu:  + Pro:  + Ser:  + Caprate:  neg Citrate:  neg Fumarate:  + DL-Glycerate:  neg 2-Ketogluconate:  + L-Malate:  + Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg β-Mannosidase:  neg ArgDH:  vr Chymotrypsin:  neg GluDC:  vr Trypsin:  neg AlanineAA:  + AlaPheProAA:  + CystineAA:  vr GluGluAA:  neg GlyAA:  + LeuAA:  + LeuGlyAA:  vr PyrrolidAA:  + ValAA:  vr AlkalineP:  vr AcidP:  vr DNAse:  neg Esterase(C4):  + EstLip(C8):  vr Lipase(C14):  neg Phosphoamidase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(MIC50): 1, MIC90: >32, RNG: (0.03–32)
    piper-taz:  S(MIC50): 4, MIC90: >128, RNG: (0.03->128)
    imipenem:  Var(MIC50): 0.06, MIC90: >32, RNG: (0.03->32)
    cefepime:  Var(MIC50): 1, MIC90: >32, RNG: (0.06–>32)
    cefotaxime:  Var(MIC50): 1, MIC90: >32, RNG: (0.03->32)
    cefoxitin:  Var(MIC50): 8, MIC90: 64, RNG: (0.125–>128)
    ceftazidime:  R(MIC50): 32, MIC90: >64, RNG: (0.5–>64)
    cephalothin:  S(30; disc)
    amikacin:  S(30; disc)
    gentamicin:  S(10; disc)
    kanamycin:  S(30; disc)
    tobramycin:  S(10; disc)
    erythromycin:  S(15; disc)
    linezolid:  S(MIC50): 0.5, MIC90: 1, RNG: (0.25–2)
    levofloxacin:  Var(MIC50): 0.25, MIC90: >16, RNG: (0.125–>16)
    nalidixic-acid:  R(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(30; disc)
    vancomycin:  S(MIC50): 0.5, MIC90: 0.5, RNG: (0.125–1)
    sulfadiazine:  R(300; disc)
    lincomycin:  R(2; disc, variable)
    daptomycin:  S(MIC50): 0.5, MIC90: 2, RNG: (0.06–2)

    References

    SPECIFIC REFERENCES FOR CORYNEBACTERIUM TUBERCULOSTEARICUM
  • Brown1984 - Description of Corynebacterium tuberculostearicum sp. nov., a leprosy-derived corynebacterium.
  • Feurer2004 - Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.
  • Bernard2012Bergey - Bergey's manual of systematic bacteriology. Vol. 5, The Actinobacteria. Part A & B. Corynebacteriaceae, Genus I. Corynebacterium
  • Tyrrell2012 - In vitro activity of TD-1792, a multivalent glycopeptide-cephalosporin antibiotic, against 377 strains of anaerobic bacteria and 34 strains of Corynebacterium species.
  • Goldstein2006a - In vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CORYNEBACTERIUM TUBERCULOSTEARICUM
  • Aujoulat2014 - Temporal dynamics of the very premature infant gut dominant microbiota.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
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    • Added: April 08, 2021