Cupriavidus metallidurans

(aka Ralstonia metallidurans)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Cupriavidus metallidurans, (aka Ralstonia metallidurans), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 63.7%. Cupriavidus metallidurans is likely to be transient and not a long-term gut coloniser. (Goris2001; Yabuuchi2005Bergey)



  • This organism has been recovered from human faeces, and wastewater. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Borderline cold mesophile, with optimum growth temp of 27C. Unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Goris2001); (Yabuuchi2005Bergey);
    Character Response
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 3.0% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 7.0-8.0;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 4℃; grows at 27℃; grows at 37℃; doesn't grow at 41℃; Grows optimally at 27℃.
  • H+
  • Acid from carbohydrates usually produced:
  • melibiose;
  • ⥅
  • Substrates assimilated or utilised:
  • gluconate; adipate; caprate; citrate; malate; L-malate; phenylacetate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; esterase C4; Leu arylamidase; oxidase; Val arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Goris2001); (Yabuuchi2005Bergey);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • Nitrite:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Yabuuchi2005Bergey);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; ampicillin; piperacillin;
  • aztreonam; imipenem; meropenem; penicillin;
  • Cephalosporins:
  • cefmetazole; cefoperazone; cefotaxime;
  • cefaclor; cefazolin; ceftazidime; moxalactam;
  • Macrolides:
  • clarithromycin; erythromycin; roxithromycin;
  • Tetracyclines:
  • doxycycline; minocycline; tetracycline;
  • Quinolines:
  • levofloxacin; ofloxacin; sparfloxacin;
  • ciprofloxacin; norfloxacin;
  • Aminoglycosides:
  • amikacin; gentamicin;
  • Heterocycles:
  • trimethoprim; trimethoprim-sulfamethoxazole;
  • NOTES

    Unable to ferment glucose.

  • McLaughlin, S. D., Walker, A. W., Churcher, C., Clark, S. K., Tekkis, P. P., Johnson, M. W., Parkhill, J., Ciclitira, P. J., Dougan, G., Nicholls, R. J., & Petrovska, L. (2010). The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing. Annals of Surgery, 252(1), 90–98.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Burkholderiaceae Genus:  Cupriavidus Alt. name:  Ralstonia metallidurans Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, and wastewater
    DNA G+C(%):  63.7
    Opt. T:  27℃
    Low T(℃):  4(neg)
    Lower T(℃):  27(+)
    Mid T(℃):  37(+)
    High T(℃):  41(neg)
    NaCl 3-5%:  3.0(neg)
    pH 6.0-8.0:  7.0-8.0(+)
    		Aesculin:  neg Urea:  neg Gelatin:  neg Starch:  neg Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fructose:  neg Galactose:  neg Glucose:  neg Rhamnose:  neg Ribose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  + Sucrose:  neg Trehalose:  neg Inulin:  neg Adonitol:  neg Dulcitol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Ethanol:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Glucose:  neg Mannose:  neg Maltose:  neg N_Acetyl_glucosamine:  neg Gluconate:  + Mannitol:  neg Lys:  neg Met:  neg Adipate:  + Caprate:  + Citrate:  + Malate:  + L-Malate:  + Phenylacetate:  d(+)
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg Trypsin:  neg CystineAA:  neg LeuAA:  + ValAA:  + AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  vr Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  S(25; disc)
    ampicillin:  S(10; disc)
    aztreonam:  R(30; disc)
    penicillin:  R(25; disc)
    piperacillin:  S(100; disc)
    imipenem:  R(10; disc)
    meropenem:  R(10; disc)
    cefaclor:  R(30; disc)
    cefazolin:  R(30; disc)
    cefmetazole:  S(30; disc)
    cefoperazone:  S(75; disc)
    cefotaxime:  S(30; disc)
    ceftazidime:  R(30; disc)
    moxalactam:  R(30; disc)
    amikacin:  R(30; disc)
    gentamicin:  R(10; disc)
    erythromycin:  R(15; disc)
    clarithromycin:  R(15; disc)
    roxithromycin:  R(15; disc)
    ciprofloxacin:  R(5; disc)
    levofloxacin:  S(5; disc)
    norfloxacin:  R(10; disc)
    ofloxacin:  S(5; disc)
    sparfloxacin:  S(5; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(10; disc)
    minocycline:  S(30; disc)
    tetracycline:  S(30; disc)
    trimethoprim:  R(5; disc)
    SXT:  R(25; disc)
    polymyxin_B:  Var(MIC50): 300; disc), MIC90: Var(300; disc

    References

    SPECIFIC REFERENCES FOR CUPRIAVIDUS METALLIDURANS
  • Goris2001 - Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al. 1998 emend.
  • Yabuuchi2005Bergey - Bergey's manual of systematic bacteriology. Vol. 2, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria Part C. Family Burkholderiaceae, Genus I. Ralstonia
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR CUPRIAVIDUS METALLIDURANS
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021