Dialister pneumosintes

(aka Bacteroides pneumosintes)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Dialister pneumosintes, (aka Bacteroides pneumosintes), is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. The DNA G+C content is 35%. Dialister pneumosintes is probably a common, although minor, coloniser of the gut. (Downes2003; JumasBilak2005; Moore1994b; Wade2011bBergey)



  • This organism has been recovered from human faeces, oral cavity and clinical sources (abscesses, infections, blood). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • GENERAL CHARACTERISTICS (Downes2003); (JumasBilak2005); (Moore1994b); (Wade2011bBergey);
    Character Response
  • 💧
  • Bile tolerance:
  • Doesn't tolerate 20% bile
  • Active enzymes:
  • Arg arylamidase; His arylamidase; Leu arylamidase;
    • SPECIAL FEATURES (Downes2003); (JumasBilak2005); (Moore1994b); (Wade2011bBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate (trace); propionate (trace); lactate (trace); succinate (trace);
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Downes2003); (JumasBilak2005); (Goldstein2006); (Goldstein2000a);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin-sulbactam; imipenem; penicillin G; ticarcillin-clavulanic acid;
  • Cephalosporins:
  • cefotaxime; ceftizoxime;
  • Macrolides:
  • azithromycin; erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • ciprofloxacin; levofloxacin;
  • Aminoglycosides:
  • kanamycin;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;
  • colistin;

  • Finegold, S. M., Howard, R. A., & Vera, L. S. (1974). Effect of diet on human intestinal fecal flora: comparison of Japanese and American diets. Am. J. Clin. Nutr, 27, 1456–1469.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Negativicutes Order:  Veillonellales Family:  Veillonellaceae Genus:  Dialister Alt. name:  Bacteroides pneumosintes Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, oral cavity and clinical sources (abscesses, infections, blood)
    DNA G+C(%):  35
    Bile reaction(%):  20(neg)
    		Aesculin:  neg Urea:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Mannose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Succinate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg GluDC:  vr AlanineAA:  neg ArgAA:  + GluGluAA:  neg GlyAA:  vr HisAA:  + LeuAA:  + LeuGlyAA:  vr PyrrolidAA:  neg PheAA:  neg TyrAA:  neg AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  trace(+) Propionate:  trace(+) Lactate:  trace(+) Succinate:  trace(+) Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (≤0.015-0.25)
    amp-sulb:  S(MIC50): 0.5, MIC90: 4, RNG: (0.06->32)
    penicillin_G:  S(MIC50): 0.125, MIC90: 0.5, RNG: (≤0.03-1)
    piper-taz:  Var(MIC50): 0.25, MIC90: >128, RNG: (0.06->128)
    tica-clav:  S(MIC50): 0.25, MIC90: 4, RNG: (0.06->128)
    imipenem:  S(MIC50): 0.125, MIC90: 0.5, RNG: (0.015–16)
    cefalexin:  Var(MIC50): 0.25, MIC90: >32, RNG: (≤0.03->32)
    cefotaxime:  S(0.5)
    cefoxitin:  Var(MIC50): 2, MIC90: 32, RNG: (0.25–>128)
    ceftizoxime:  S(0.06)
    kanamycin:  S(30; disc)
    azithromycin:  S(MIC50): 0.125, MIC90: 0.25, RNG: (≤0.03-0.25)
    erythromycin:  S(MIC50): 0.125, MIC90: 2, RNG: (≤0.03-2)
    ciprofloxacin:  S(MIC50): ≤0.5, MIC90: 1, RNG: (≤0.5->8)
    levofloxacin:  S(MIC50): ≤0.06, MIC90: 0.5, RNG: (≤0.06->8)
    moxifloxacin:  Var(MIC50): 0.25, MIC90: 4, RNG: (0.06–>16)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(MIC50): 0.25, MIC90: 2, RNG: (0.06-4)
    vancomycin:  R(5; disc)
    chloramphenicol:  S(MIC50): 2, MIC90: 8, RNG: (0.25–32)
    metronidazole:  S(MIC50): 0.125, MIC90: 4, RNG: (≤0.03-4)
    clindamycin:  S(MIC50): ≤0.03, MIC90: 2, RNG: (≤0.03-2)
    colistin:  R(10; disc)

    References

    SPECIFIC REFERENCES FOR DIALISTER PNEUMOSINTES
  • Downes2003 - Dialister invisus sp. nov., isolated from the human oral cavity.
  • JumasBilak2005 - Dialister micraerophilus sp. nov. and Dialister propionicifaciens sp. nov., isolated from human clinical samples.
  • Moore1994b - Oribaculum catoniae gen. nov., sp. nov.: Catonella morbi gen. nov., sp. nov.: Hallella seregens gen. nov., sp. nov.: Johnsonella ignava gen. nov., sp. nov.: and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic Gram-Negative Bacilli from the Human Gingival Crevice.
  • Wade2011bBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Veillonellaceae, Genus XII. Dialister
  • Weir2013 - Stool microbiome and metabolome differences between colorectal cancer patients and healthy adults
  • Goldstein2006 - In vitro activity of moxifloxacin against 923 anaerobes isolated from human intra-abdominal infections.
  • Goldstein2000a - Comparative In vitro activities of ertapenem (MK-0826) against 1,001 anaerobes isolated from human intra-abdominal infections.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR DIALISTER PNEUMOSINTES
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Finegold1974 - Effect of diet on human fecal flora: comparison of Japanese and American diets
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • ...............................
    • GENERAL REFERENCES FOR DIALISTER PNEUMOSINTES
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021