Dietzia natronolimnaea

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Dietzia natronolimnaea is a Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 66.1%. Dietzia natronolimnaea is probably a rare gut coloniser. (Duckworth1998)



  • This organism has been recovered from an alkaline lake and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Duckworth1998);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • pH
  • Acidity tolerance:
  • tolerates pH 6; tolerates pH 10; Grows optimally at pH 9.
  • 🌡
  • Temperature tolerance:
  • grows weakly at 10℃; grows at 20℃; grows at 40℃; grows weakly at 45℃; Grows optimally at 30℃.
  • ⥅
  • Substrates assimilated or utilised:
  • fructose; glucose; mannitol; arginine; glutamate; glycine; methionine; phenylalanine; serine; valine; acetate; citrate; fumarate; 3-hydroxybutyrate; propionate; suberate; succinate; valerate; glutamine;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; esterase C4; esterase lipase C8; α-glucosidase; Leu arylamidase; pyrrolidine arylamidase;
    • SPECIAL FEATURES
    Character Response
  • ✓
  • Nitrate:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Duckworth1998);
    Class Active Resistant
  • Penicillins:
  • methicillin;
  • penicillin G;
  • Macrolides:
  • erythromycin; oleandomycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • neomycin; streptomycin;
  • kanamycin;
  • Polypep/ketides:
  • bacitracin; rifampicin;
  • Heterocycles:
  • chloramphenicol; fusidic-acid;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • novobiocin; polymyxin B;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Mycobacteriales Family:  Dietziaceae Genus:  Dietzia Gram stain:  + Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  an alkaline lake and human faeces
    DNA G+C(%):  66.1
    Opt. T:  30℃
    Low T(℃):  10(w)
    Lower T(℃):  20(+)
    Mid T(℃):  40(+)
    High T(℃):  45(w)
    Opt. pH:  9
    pH 6.0-8.0:  6(+)
    pH >8:  10(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg Ribose:  neg Xylose:  neg Lactose:  neg Maltose:  neg Glycogen:  neg Mannitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  + Fucose:  neg Galactose:  neg Glucose:  + Lactose:  neg Melibiose:  neg Sucrose:  neg Inositol:  neg Mannitol:  + Salicin:  neg Sorbitol:  neg Ala:  neg Arg:  + Glu:  + Gly:  + His:  neg Lys:  neg Met:  + Phe:  + Ser:  + Val:  + Acetate:  + Caprate:  neg Citrate:  + Fumarate:  + 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Itaconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg DL-Lactate:  neg Malonate:  neg Propionate:  + Suberate:  + Succinate:  + Valerate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg Chymotrypsin:  vr Trypsin:  vr CystineAA:  neg LeuAA:  + PyrrolidAA:  + ValAA:  vr AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  + Lipase(C14):  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  Var(MIC50): 25; disc), MIC90: Var(25; disc
    methicillin:  S(10; disc)
    penicillin_G:  R(1U)
    gentamicin:  Var(MIC50): 10; disc), MIC90: Var(10; disc
    kanamycin:  R(30; disc)
    neomycin:  S(30; disc)
    streptomycin:  S(10; disc)
    erythromycin:  S(5; disc)
    oleandomycin:  S(5; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(25; disc)
    vancomycin:  S(30; disc)
    bacitracin:  S(10U)
    rifampicin:  S(2; disc)
    chloramphenicol:  S(25; disc)
    novobiocin:  R(5; disc)
    polymyxin_B:  R(300U)
    fusidic-acid:  S(10; disc)

    References

    SPECIFIC REFERENCES FOR DIETZIA NATRONOLIMNAEA
  • Duckworth1998 - Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR DIETZIA NATRONOLIMNAEA
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021