Enterobacter massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Enterobacter massiliensis is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 55.1%. Enterobacter massiliensis is probably a rare gut coloniser. (Lagier2013)



  • This organism has been recovered from human faeces. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal. Robust growth can have positive consequences for gut health.
    • GENERAL CHARACTERISTICS (Lagier2013);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 6.5% salt;
  • 🌡
  • Temperature tolerance:
  • grows weakly at 10℃; grows at 25℃; grows at 37℃; grows weakly at 45℃; Grows optimally at 30-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; rhamnose; amygdalin; cellubiose; gentiobiose; lactose; maltose; trehalose; mannitol; sorbitol; arbutin; β-methyl glucoside; N-Ac glucosamine;
  • Active enzymes:
  • N-Ac β-glucosaminidase; catalase; β-galactosidase; β-glucosidase; Leu arylamidase; β-mannosidase;
    • SPECIAL FEATURES (Lagier2013);
    Character Response
  • ✓
  • Metabolites produced:
  • indole;
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS (Lagier2013);
    Class Active Resistant
  • Penicillins:
  • imipenem; ticarcillin;
  • Aminoglycosides:
  • amikacin; gentamicin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;
  • fosfomycin; nitrofurantoin;
  • Miscellaneous antibiotics:
  • colistin;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Enterobacterales Family:  Enterobacteriaceae Genus:  Enterobacter Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  neg
    Health:   Positive
    Source:  human faeces
    DNA G+C(%):  55.1
    Opt. T:  30-37℃
    Low T(℃):  10(w)
    Lower T(℃):  25(+)
    Mid T(℃):  37(+)
    High T(℃):  45(w)
    NaCl >6%:  6.5(neg)
    		Aesculin:  + Urea:  neg Starch:  neg Hippurate:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  w(+) Ribose:  neg Sorbose:  neg D-Tagatose:  neg L-Xylose:  neg Cellubiose:  + Gentiobiose:  + Lactose:  + Maltose:  + Melezitose:  neg Sucrose:  neg Trehalose:  + Amygdalin:  + Dextrin:  neg Glycogen:  neg Inulin:  neg Adonitol:  neg D-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  + Sorbitol:  + Xylitol:  neg Arbutin:  + Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg NAc-α-GA:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg Ac-β-glcamnd:  + α-Galactosidase:  neg β-Galactosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg β-Mannosidase:  + ArgDH:  neg AlaPheProAA:  neg LeuAA:  + PyrrolidAA:  neg AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  + Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ticarcillin:  Sens
    imipenem:  Sens
    amikacin:  Sens
    gentamicin:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    fosfomycin:  Res
    nitrofurantoin:  Res
    SXT:  Sens
    colistin:  Sens

    References

    SPECIFIC REFERENCES FOR ENTEROBACTER MASSILIENSIS
  • Lagier2013 - Non contiguous-finished genome sequence and description of Enterobacter massiliensis sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ENTEROBACTER MASSILIENSIS
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021