Ethanoligenens harbinense

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Ethanoligenens harbinense is a Gram-positive, non-spore-forming, strictly anaerobic, motile, rod-shaped bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 47.8%. Ethanoligenens harbinense is probably a rare gut coloniser. (Xing2006)



  • This organism has been recovered from wastewater. Has been detected in human faeces (New2022). The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Xing2006);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; gelatin; urea;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • milk;
  • 🧂
  • Salt tolerance:
  • tolerates 0.5-2.0% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 3.5; tolerates pH 6.0-8.0; tolerates pH 9; Grows optimally at pH 4.5-5.0.
  • 🌡
  • Temperature tolerance:
  • grows at 20℃; grows at 44℃; Grows optimally at 35℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; ribose; cellubiose; lactose; maltose; melibiose; raffinose; sucrose; trehalose; D-turanose; salicin;
  • Active enzymes:
  • N-Ac β-glucosaminidase; chymotrypsin; esterase C4; esterase lipase C8; fucosidase; β-glucuronidase; lipase C14; α-mannosidase; trypsin; urease; Val arylamidase;
    • SPECIAL FEATURES (Xing2006);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; ethanolMajor; H₂; indole;
  • ✗
  • Metabolites not produced:
  • H₂S;
  • ✓
  • Methyl red test:
  • active
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Sulfate:
  • not reduced
  • ✗
  • Thiosulfate:
  • not reduced

  • N/A

  • E. harbinense hydrolyses gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. Fermentation end products formed from glucose are acetate, ethanol, hydrogen and carbon dioxide. [UP000001551]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    Ethanoligenens harbinense (strain DSM 18485 / JCM 12961 / CGMCC 1.5033 / YUAN-3) is a strictly anaerobic, motile, chemo-organotrophic Gram-positive bacterium isolated from anaerobic activated sludge of molasses wastewater in a continuous stirred-tank reactor. Cells are regular rods and occur singly, in pairs and sometimes in chains of up to eight. Autoaggregative and autofluorescent growth is observed at 20-44 degrees Celsius and at pH between 3.5-9.0, with optimum growth at 35 degrees Celsius and approximately at pH 4.5-5-0. It is able to grow in the presence of 0-2% (w/v) NaCl. E. harbinense hydrolyses gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. Fermentation end products formed from glucose are acetate, ethanol, hydrogen and carbon dioxide. (Adapted from PMID: 16585689). [UP000001551]

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Oscillospiraceae Genus:  Ethanoligenens Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  wastewater. Has been detected in human faeces (New2022)
    DNA G+C(%):  47.8
    Opt. T:  35℃
    Lower T(℃):  20(+)
    High T(℃):  44(+)
    NaCl 0.5-2%:  0.5-2.0(+)
    Opt. pH:  4.5-5.0
    pH 4.2-5.9:  3.5(+)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  9(+)
    		Aesculin:  + Urea:  + Gelatin:  + Starch:  w Milk:  curdle
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  neg Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  neg Cellubiose:  + Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  + Sucrose:  + Trehalose:  + Turanose:  + Glycogen:  neg Inulin:  neg Starch:  w Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Inositol:  neg Mannitol:  neg Xylitol:  neg Ethanol:  neg Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Ethanol:  neg Methanol:  neg Propanol:  neg Xylan:  neg Hippurate:  neg Fumarate:  neg 3-Hydroxybutyrate:  neg Malate:  neg Malonate:  neg Phenylacetate:  neg Pyruvate:  neg Succinate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  + Ac-β-glcamnd:  + α-Fucosidase:  + α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucuronidase:  + α-Mannosidase:  + ArgDH:  neg Chymotrypsin:  + Trypsin:  + CystineAA:  neg ValAA:  + AlkalineP:  neg AcidP:  neg Esterase(C4):  + EstLip(C8):  + Lecithinase:  neg Lipase:  neg Lipase(C14):  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Ethanol:  Major H2S:  neg H2:  + Indole:  +

    References

    SPECIFIC REFERENCES FOR ETHANOLIGENENS HARBINENSE
  • Xing2006 - Ethanoligenens harbinense gen. nov., sp. nov., isolated from molasses wastewater.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ETHANOLIGENENS HARBINENSE
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • Qin2012 - Metagenome-wide association study of gut microbiota in type 2 diabetes
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR ETHANOLIGENENS HARBINENSE
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: February 08, 2021