Eubacterium tenue

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Eubacterium tenue is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. Eubacterium tenue is probably a common, although minor, coloniser of the gut. (Wade2011aBergeys)



  • This organism has been recovered from clinical sources (abscess, knee synovial fluid, blood) and human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Wade2011aBergeys);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; gelatin;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • milk;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 6.5% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 24℃; grows at 37℃;
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; maltose;
  • Active enzymes:
  • alkaline phosphatase; fucosidase; β-galactosidase; α-glucosidase; lecithinase;
  • ±
  • Strain-dependent active enzymes:
  • pyrrolidine arylamidase;
    • SPECIAL FEATURES (Wade2011aBergeys);
    Character Response
  • ✓
  • Metabolites produced:
  • formate; acetate; propionate (minor); isobutyrate (minor); ethanolminor; propanolminor; butanolminor; isovalerate (minor); H₂S; ammonia; H₂; indole;
  • ✗
  • Metabolites not produced:
  • butyrate;
  • ✗
  • VP test:
  • not active
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Wade2011aBergeys); (Goldstein2005);
    Class Active Resistant
  • Penicillins:
  • ampicillin; imipenem; penicillin; penicillin G; piperacillin-tazobactam;
  • Macrolides:
  • pristinamycin; quinupristin-dalfopristin;
  • Heterocycles:
  • metronidazole;
  • Vancomycins:
  • vancomycin; dalbavancin;
  • Miscellaneous antibiotics:
  • clindamycin; daptomycin; pristinamycin;

  • Distaso, A (1911). Sur les microbes protéolytiques de la flore intestinale de l'homme et des animaux. Zbl. Bakt. Parasit. 59: 97-103.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Eubacteriaceae Genus:  Eubacterium Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  clinical sources (abscess, knee synovial fluid, blood) and human faeces
    Lower T(℃):  24(+)
    Mid T(℃):  37(+)
    NaCl >6%:  6.5(neg)
    		Aesculin:  + Urea:  neg Gelatin:  + Starch:  neg Hippurate:  neg Milk:  d Meat:  vr
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Fructose:  neg Glucose:  w(+) Mannose:  neg Rhamnose:  neg Ribose:  neg Cellubiose:  neg Lactose:  neg Maltose:  w(+) Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Amygdalin:  neg Aesculin:  neg Glycogen:  neg Starch:  neg Mannitol:  neg Sorbitol:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  + α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  neg GluGluAA:  neg GlyAA:  neg LeuAA:  neg LeuGlyAA:  neg PyrrolidAA:  d AlkalineP:  + Lecithinase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  + Acetate:  + Propionate:  minor(+) Butyrate:  neg Isobutyrate:  minor(+) Ethanol:  minor Propanol:  minor Butanol:  minor Isovalerate:  minor(+) H2S:  + Ammonia:  + H2:  + Indole:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(MIC50): 0.03, MIC90: 0.06, RNG: (0.03-0.125)
    penicillin:  S(MIC50): 0.125, MIC90: 2, RNG: (0.02-2)
    penicillin_G:  S(2U)
    piper-taz:  S(MIC50): ≤0.03, MIC90: 0.5, RNG: (≤0.03-0.5)
    imipenem:  S(MIC50): 0.03, MIC90: 0.5, RNG: (≤0.015-0.5)
    clarithromycin:  Var(MIC50): 0.5, MIC90: >64, RNG: (0.03–>64)
    pristinamycin:  S(MIC50): 0.12, MIC90: 1, RNG: (0.03–1)
    quin-dalf:  S(MIC50): 0.5, MIC90: 1, RNG: (≤0.03-1)
    telithromycin:  Var(MIC50): 0.12, MIC90: >32, RNG: (0.03->32)
    linezolid:  Var(MIC50): 2, MIC90: 4, RNG: (0.25-8)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    dalbavancin:  S(MIC50): 0.25, MIC90: 1, RNG: (0.06-2)
    vancomycin:  S(MIC50): 0.5, MIC90: 2, RNG: (0.25-2)
    metronidazole:  S(MIC50): 0.25, MIC90: 1, RNG: (0.02-1)
    clindamycin:  S(MIC50): 0.06, MIC90: 0.5, RNG: (0.02-1)
    daptomycin:  S(MIC50): 0.5, MIC90: 0.5, RNG: (0.06-8)

    References

    SPECIFIC REFERENCES FOR EUBACTERIUM TENUE
  • Wade2011aBergeys - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Eubacteriaceae, Genus I. Eubacterium
  • Goldstein2005 - Comparative in vitro activities of XRP 2868, pristinamycin, quinupristin-dalfopristin, vancomycin, daptomycin, linezolid, clarithromycin, telithromycin, clindamycin, and ampicillin against anaerobic gram-positive species, actinomycetes, and lactobacilli.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR EUBACTERIUM TENUE
  • Finegold1974 - Effect of diet on human fecal flora: comparison of Japanese and American diets
  • Finegold1977 - Fecal microbial flora in Seventh Day Adventist populations and control subjects.
  • Holdeman1976 - Human fecal flora: variation in bacterial composition within individuals and a possible effect of emotional stress.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Nam2008a - Bacterial, archaeal, and eukaryal diversity in the intestines of Korean people.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR EUBACTERIUM TENUE
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021