Fusobacterium massiliense

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Fusobacterium massiliense is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 27%. Fusobacterium massiliense is probably a rare gut coloniser. (Mailhe2016c)



  • This organism has been recovered from duodenum of a patient with iron deficiency. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Mailhe2016c);
    Character Response
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 0.5% salt; Grows optimally with 0% salt.
  • pH
  • Acidity tolerance:
  • tolerates pH 5.5; tolerates pH 8.0;
  • 🌡
  • Temperature tolerance:
  • grows at 28℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; D-tagatose; aesculin; gentiobiose; raffinose; 5-ketogluconate;
  • ⥅
  • Substrates assimilated or utilised:
  • aesculin; raffinose;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; chymotrypsin; esterase C4; esterase lipase C8;
    • SPECIAL FEATURES (Mailhe2016c);
    Character Response
  • ✓
  • Metabolites produced:
  • indole;
    • RESPONSE TO ANTIBIOTICS (Mailhe2016c);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; amoxicillin-clavulanic acid; imipenem; oxacillin; penicillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline;
  • Aminoglycosides:
  • tobramycin;
  • gentamicin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • metronidazole;
  • fosfomycin; trimethoprim-sulfamethoxazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; colistin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Fusobacteria Class:  Fusobacteriia Order:  Fusobacteriales Family:  Fusobacteriaceae Genus:  Fusobacterium Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  duodenum of a patient with iron deficiency
    DNA G+C(%):  27
    Opt. T:  37℃
    Lower T(℃):  28(+)
    NaCl 0.5-2%:  0.5(neg)
    pH 4.2-5.9:  5.5(+)
    pH 6.0-8.0:  8.0(+)
    		Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  + Fructose:  neg Glucose:  neg Mannose:  neg D-Tagatose:  + Gentiobiose:  + Lactose:  neg Maltose:  neg Aesculin:  + 5-Ketogluconate:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Fructose:  neg Glucose:  neg Mannose:  neg Lactose:  neg Maltose:  neg Raffinose:  + Aesculin:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg β-Galactosidase:  neg β-Glucuronidase:  neg Chymotrypsin:  + AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  S(25; disc)
    Augmentin:  S(30; disc)
    oxacillin:  S(5; disc)
    penicillin:  S(10U)
    imipenem:  S(10; disc)
    gentamicin:  R(15; disc)
    tobramycin:  S(10; disc)
    erythromycin:  R(15; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(30; disc)
    vancomycin:  R(30; disc)
    rifampicin:  S(30; disc)
    fosfomycin:  R(50; disc)
    metronidazole:  S(4; disc)
    SXT:  R(25; disc)
    clindamycin:  S(15; disc)
    colistin:  S(50; disc)

    References

    SPECIFIC REFERENCES FOR FUSOBACTERIUM MASSILIENSE
  • Mailhe2016c - Noncontiguous finished genome sequence and description of Fusobacterium massiliense sp. nov. isolated from human duodenum.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR FUSOBACTERIUM MASSILIENSE
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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    • Added: January 14, 2022