Fusobacterium perfoetens

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Fusobacterium perfoetens is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, oval-shaped bacterium. The DNA G+C content is 28-30%. Fusobacterium perfoetens is probably a rare gut coloniser. (Gharbia2010Bergey; VanAssche1977)



  • This organism has been recovered from an infant with diarrhoea, and piglet faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Gharbia2010Bergey); (VanAssche1977);
    Character Response
  • 💧
  • Bile tolerance:
  • Sensitive
  • pH
  • Acidity tolerance:
  • tolerates pH 5.6;
  • 🌡
  • Temperature tolerance:
  • grows weakly at 30℃; grows at 45℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; sucrose;
  • ⥅
  • Substrates assimilated or utilised:
  • threonine;
  • Active enzymes:
  • N-Ac β-glucosaminidase; β-galactosidase;
    • SPECIAL FEATURES (Gharbia2010Bergey); (VanAssche1977);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate (trace); butyrate; lactate; H₂S; ammonia; CO₂;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • VP test:
  • not active
  • Haemolysis:
  • beta(absent)
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (VanAssche1977);
    Class Active Resistant
  • Penicillins:
  • ampicillin; carbenicillin; penicillin;
  • Macrolides:
  • erythromycin; oleandomycin;
  • Tetracyclines:
  • chlortetracycline; oxytetracycline;
  • Aminoglycosides:
  • kanamycin; neomycin;
  • Polypep/ketides:
  • bacitracin;
  • Heterocycles:
  • chloramphenicol;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Fusobacteria Class:  Fusobacteriia Order:  Fusobacteriales Family:  Fusobacteriaceae Genus:  Fusobacterium Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Oval-shaped
    Health:  Unknown
    Source:  an infant with diarrhoea, and piglet faeces
    DNA G+C(%):  28-30
    Opt. T:  37℃
    Lower T(℃):  30(w)
    High T(℃):  45(+)
    pH 4.2-5.9:  5.6(+)
    Bile reaction(%):  S
    		Aesculin:  neg Gelatin:  neg Milk:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  w Galactose:  w Glucose:  + Mannose:  w Rhamnose:  neg Ribose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Melibiose:  neg Sucrose:  + Trehalose:  w Amygdalin:  neg Dextrin:  neg Glycerol:  neg Mannitol:  neg Sorbitol:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Fructose:  w Glucose:  w Mannose:  neg Lactose:  neg Maltose:  neg Raffinose:  neg Sucrose:  w Thr:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Ac-β-glcamnd:  + α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg AlkalineP:  neg AcidP:  w Esterase(C4):  neg EstLip(C8):  neg Lecithinase:  neg Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Propionate:  trace(+) Butyrate:  + Lactate:  + H2S:  + Ammonia:  + CO2:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  R(10; disc)
    carbenicil:  R(50; disc)
    penicillin:  R(10U)
    kanamycin:  R(30; disc)
    neomycin:  R(30; disc)
    erythromycin:  R(15; disc)
    oleandomycin:  R(15; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    chlortetracycline:  S(30; disc)
    oxytetracycline:  S(30; disc)
    vancomycin:  R(30; disc)
    bacitracin:  R(10; disc)
    rifampicin:  Var(MIC50): 30; disc), MIC90: Var(30; disc
    chloramphenicol:  S(30; disc)
    clindamycin:  S(2; disc)
    colistin:  Var(MIC50): 10; disc), MIC90: Var(10; disc
    polymyxin_B:  Var(MIC50): 300U), MIC90: Var(300U

    References

    SPECIFIC REFERENCES FOR FUSOBACTERIUM PERFOETENS
  • Gharbia2010Bergey - Bergey's manual of systematic bacteriology. Vol. 4, The Fusobacteria. Family Fusobacteriaceae, Genus I. Fusobacterium
  • Saito2019 - Metagenomic analyses of the gut microbiota associated with colorectal adenoma
  • VanAssche1977 - Fusobacterium perfoetens (Tissier) Moore and Holdeman 1973: Description and Proposed Neotype Strain
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    • Added: September 19, 2022