Gracilibacillus massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Gracilibacillus massiliensis is a Gram-positive, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 36%. Gracilibacillus massiliensis is probably a rare gut coloniser. (Diop2016)



  • This organism has been recovered from cooking salt specimens. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Diop2016);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; urea;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 0% salt; tolerates 7.5% salt;
  • pH
  • Acidity tolerance:
  • Grows optimally at pH 7.0-8.0.
  • 🌡
  • Temperature tolerance:
  • Grows optimally at 37℃.
  • Active enzymes:
  • N-Ac β-glucosaminidase; catalase; esterase; α-galactosidase; β-glucosidase; β-glucuronidase; lipase; urease;
    • SPECIAL FEATURES (Diop2016);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Diop2016);
    Class Active Resistant
  • Penicillins:
  • imipenem;
  • amoxicillin; penicillin G;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline;
  • Quinolines:
  • ciprofloxacin;
  • Aminoglycosides:
  • gentamicin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;
  • metronidazole;
  • Vancomycins:
  • vancomycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Gracilibacillus Gram stain:  + O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  cooking salt specimens
    DNA G+C(%):  36
    Opt. T:  37℃
    NaCl 0.5-2%:  0(neg)
    NaCl >6%:  7.5(+)
    Opt. pH:  7.0-8.0
    		Aesculin:  + Urea:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Fructose:  neg Glucose:  neg Mannose:  neg Ribose:  neg Lactose:  neg Maltose:  neg Sucrose:  neg Glycerol:  neg Mannitol:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  + Ac-β-glcamnd:  + α-Galactosidase:  + β-Galactosidase:  neg β-Glucosidase:  + β-Glucuronidase:  + AlkalineP:  neg Esterase:  + Lipase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  Res
    penicillin_G:  Res
    imipenem:  Sens
    gentamicin:  Sens
    erythromycin:  Sens
    ciprofloxacin:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  Sens
    vancomycin:  Sens
    rifampicin:  Sens
    metronidazole:  Res
    SXT:  Sens

    References

    SPECIFIC REFERENCES FOR GRACILIBACILLUS MASSILIENSIS
  • Diop2016 - Microbial culturomics unravels the halophilic microbiota repertoire of table salt: description of Gracilibacillus massiliensis sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR GRACILIBACILLUS MASSILIENSIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • ...............................
    • Added: January 14, 2022