Grimontia hollisae

(aka Vibrio hollisae)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Grimontia hollisae, (aka Vibrio hollisae), is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 49.3-51.0%. Grimontia hollisae is probably a rare gut coloniser. (Thompson2003; Hickman1982; Farmer2005gBergey)



  • This organism has been recovered from human faeces and is a likely cause of diarrhoea. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread (notes: zoonotic pathogen). It is an opportunistic pathogen. A possible gut commensal.
    • QUIRKS
  • Requires Na+ for growth.
    • GENERAL CHARACTERISTICS (Thompson2003); (Hickman1982); (Farmer2005gBergey);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 1% salt; tolerates 5% salt; strain-variable at 6(d)%;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 36℃;
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; galactose; glucose; mannose;
  • ⥅
  • Substrates assimilated or utilised:
  • L-arabinose; galactose; mannose;
  • Active enzymes:
  • oxidase;
    • SPECIAL FEATURES (Thompson2003); (Hickman1982); (Farmer2005gBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • indole;
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✗
  • ONPG test:
  • not active
  • KCN growth:
  • inhibited
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Farmer2005gBergey);
    Class Active Resistant
  • Penicillins:
  • ampicillin; carbenicillin; penicillin;
  • Cephalosporins:
  • cephalothin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; kanamycin; streptomycin;
  • Heterocycles:
  • chloramphenicol; sulfadiazine;
  • Miscellaneous antibiotics:
  • colistin; polymyxin B;

  • Hickman, F. W., Farmer, J. J., 3rd, Hollis, D. G., Fanning, G. R., Steigerwalt, A. G., Weaver, R. E., & Brenner, D. J. (1982). Identification of Vibrio hollisae sp. nov. from patients with diarrhea. Journal of Clinical Microbiology, 15(3), 395–401.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Vibrionales Family:  Vibrionaceae Genus:  Grimontia Alt. name:  Vibrio hollisae Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and is a likely cause of diarrhoea
    DNA G+C(%):  49.3-51.0
    Lower T(℃):  25(+)
    Mid T(℃):  36(+)
    NaCl 0.5-2%:  1(+)
    NaCl 3-5%:  5(+)
    NaCl >6%:  6(d)
    		Aesculin:  neg Urea:  neg Gelatin:  neg DNA:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Amygdalin:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  + Galactose:  + Mannose:  + Xylose:  neg Cellubiose:  neg Lactose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Inositol:  neg Mannitol:  neg Salicin:  neg Sorbitol:  neg Tyr:  neg Acetate:  neg Citrate:  neg Malonate:  neg Mucate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Urease:  neg Ac-β-glcamnd:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  neg ArgDC:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg DNAse:  neg Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(10; disc)
    carbenicil:  S(100; disc)
    penicillin:  S(10U)
    cephalothin:  S(30; disc)
    gentamicin:  S(10; disc)
    kanamycin:  S(30; disc)
    streptomycin:  S(10; disc)
    nalidixic-acid:  S(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(30; disc)
    chloramphenicol:  S(30; disc)
    sulfadiazine:  S(250; disc)
    colistin:  S(10; disc)
    polymyxin_B:  S(50U)

    References

    SPECIFIC REFERENCES FOR GRIMONTIA HOLLISAE
  • Thompson2003 - Reclassification of Vibrio hollisae as Grimontia hollisae gen. nov., comb. nov.
  • Hickman1982 - Identification of Vibrio hollisae sp. nov. from patients with diarrhea.
  • Farmer2005gBergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Vibrionaceae, Genus I. Vibrio
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR GRIMONTIA HOLLISAE
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR GRIMONTIA HOLLISAE
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: April 08, 2021