Holdemania massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Holdemania massiliensis is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. The DNA G+C content is 47.1%. Holdemania massiliensis is probably a common, although minor, coloniser of the gut. (Mishra2013b; Pfleiderer2013; Pfleiderer2018)



  • This organism has been recovered from human faeces (anorexia nervosa patient). Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Mishra2013b); (Pfleiderer2013); (Pfleiderer2018);
    Character Response
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; ribose; D-tagatose; amygdalin; aesculin; cellubiose; gentiobiose; lactose; maltose; melezitose; sucrose; glycerol; inositol; mannitol; sorbitol; arbutin; gluconate; N-Ac glucosamine; salicin;
  • Active enzymes:
  • fucosidase; β-galactosidase; α-glucosidase; oxidase; pyroGlu arylamidase;
    • SPECIAL FEATURES (Mishra2013b);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • Haemolysis:
  • alpha
  • ✗
  • Nitrate:
  • not reduced
  • ✓
  • Pigments:
  • produced
    • RESPONSE TO ANTIBIOTICS (Mishra2013b);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; imipenem;
  • Heterocycles:
  • metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;

  • Pfleiderer, A., Lagier, J.-C., Armougom, F., Robert, C., Vialettes, B., & Raoult, D. (2013). Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample. European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology, 32(11), 1471–1481.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Erysipelotrichia Order:  Erysipelotrichales Family:  Erysipelotrichaceae Genus:  Holdemania Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod Pigment:  +
    Health:  Unknown
    Source:  human faeces (anorexia nervosa patient)
    DNA G+C(%):  47.1
    Opt. T:  37℃
    Lower T(℃):  25(+)
    		Urea:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  + Galactose:  + Glucose:  + Mannose:  + Ribose:  + D-Tagatose:  + Cellubiose:  + Gentiobiose:  + Lactose:  + Maltose:  + Melezitose:  + Sucrose:  + Amygdalin:  + Aesculin:  + Glycerol:  + Inositol:  + Mannitol:  + Sorbitol:  + Arbutin:  + Gluconate:  + NAc-α-GA:  + Salicin:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  + α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  neg GluGluAA:  neg GlyAA:  neg LeuAA:  neg LeuGlyAA:  vr PyrogluAA:  + AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg Pigment:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  Sens
    imipenem:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    vancomycin:  Sens
    metronidazole:  Sens
    clindamycin:  Sens

    References

    SPECIFIC REFERENCES FOR HOLDEMANIA MASSILIENSIS
  • Mishra2013b - Non-contiguous finished genome sequence and description of Holdemania massiliensis sp. nov.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • Pfleiderer2018 - Diversität Der Intestinalen Mikrobiota Am Beispiel Der Darmflora Einer Anorexia Patientin.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR HOLDEMANIA MASSILIENSIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Jeong2021 - The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR HOLDEMANIA MASSILIENSIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021