Hydrogenophaga pseudoflava

(aka Pseudomonas pseudoflava)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Hydrogenophaga pseudoflava, (aka Pseudomonas pseudoflava), is a Gram-negative, non-spore-forming, strictly aerobic, motile, oval-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 66.5-68.0%. Hydrogenophaga pseudoflava is likely to be transient and not a long-term gut coloniser. (Willems1989; Auling1978)



  • This organism has been recovered from soil, mud and water. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Autotrophic, using H2/CO2/O2 only. Also Heterotrophic using >42 different organic substrates. Obligate aerobe; unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Willems1989); (Auling1978);
    Character Response
  • 🌡
  • Temperature tolerance:
  • Grows optimally at 35-38℃.
  • H+
  • Acid from carbohydrates usually produced:
  • arabinose; fructose; galactose; glucose; mannose; xylose; cellubiose; maltose; sucrose; trehalose;
  • ⥅
  • Substrates assimilated or utilised:
  • L-arabinose; d_arabitol; cellubiose; ethanolamine; fructose; galactose; gluconate; glucose; lactose; maltose; mannitol; mannose; sorbitol; sucrose; trehalose; D-turanose; xylose; alanine; D-alanine; γ-aminobutyrate; δ-aminovalerate; glutamate; histidine; isoleucine; leucine; lysine; ornithine; phenylalanine; proline; tryptophan; tyrosine; valine; acetate; aconitate; azelate; caproate; fumarate; glycolate; 3-hydroxybutyrate; 3-hydroxybenzoate; 4-hydroxybenzoate; lactate; DL-lactate; L-malate; phenol; pyruvate; quinate; glutamine; L-asparagine; D-malate; benzylamine; butylamine;
  • ±
  • Strain-dependent substrate utilisation:
  • ethanol; inositol; D-lyxose; propanol; rhamnose; 2-aminobutyrate; arginine; aspartate; glycine; norleucine; threonine; butyrate; citrate; DL-glycerate; isovalerate; L-mandelate; pimelate; saccharate; suberate; D-tartrate; L-tartrate; valerate; butanol; norvaline;
  • Active enzymes:
  • oxidase;
  • ±
  • Strain-dependent active enzymes:
  • alkaline phosphatase; α-glucosidase; β-glucosidase; lipase C14;
    • SPECIAL FEATURES (Auling1978);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✓
  • Nitrite:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • Pigments:
  • yellow
    • RESPONSE TO ANTIBIOTICS (Auling1978);
    Class Active Resistant
  • Penicillins:
  • ampicillin; methicillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • kanamycin; neomycin; streptomycin;
  • Heterocycles:
  • chloramphenicol;
  • Miscellaneous antibiotics:
  • novobiocin; polymyxin B;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Comamonadaceae Genus:  Hydrogenophaga Alt. name:  Pseudomonas pseudoflava Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Oval-shaped Pigment:  yellow
    Health:  Unknown
    Source:  soil, mud and water
    DNA G+C(%):  66.5-68.0
    Opt. T:  35-38℃
    		Aesculin:  neg Urea:  neg Gelatin:  neg Starch:  neg Casein:  neg Tween:  80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  + Fructose:  + Fucose:  neg Galactose:  + Glucose:  + Mannose:  + Ribose:  neg Xylose:  + Cellubiose:  + Maltose:  + Sucrose:  + Trehalose:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  + Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + D-Lyxose:  d Mannose:  + Rhamnose:  d Ribose:  neg D-Tagatose:  neg Xylose:  + Cellubiose:  + Gentiobiose:  neg Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  + D-Turanose:  + Trehalose:  + N_Acetyl_glucosamine:  neg Adonitol:  neg Amygdalin:  neg D-Arabitol:  + L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  neg Aesculin:  neg Ethanol:  d Ethanolamine:  + Gluconate:  + Glycogen:  neg Inositol:  d Inulin:  neg Mannitol:  + Me-α-D-Glc:  neg Propanol:  d Sorbitol:  + Starch:  neg Xylitol:  neg Ala:  + D-Ala:  + β-Ala:  neg 4-Aminobutyrate:  + 5-Aminovalerate:  + Arg:  d Asp:  d Betaine:  neg L-Citrulline:  neg Cys:  neg Glu:  + Gly:  d Histamine:  neg His:  + Ile:  d(+) DL-Kynurenine:  neg Leu:  + Lys:  + Met:  neg Norleucine:  d(neg) Orn:  + Phe:  + Pro:  + Sarcosine:  neg Thr:  d Trigonelline:  neg Tryptamine:  neg Trp:  + Tyr:  + Val:  d(+) Acetate:  + Aconitate:  cis(+) Adipate:  neg Azelate:  + Benzoate:  neg Butyrate:  d Caprate:  neg Caproate:  d(+) Caprylate:  neg Citraconate:  neg Citrate:  d Formate:  neg Fumarate:  + DL-Glycerate:  d Glycolate:  + Heptanoate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  d(+) 4-Hydroxybenzoate:  + Isobutyrate:  neg Isovalerate:  d Itaconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Lactate:  + DL-Lactate:  + L-Malate:  + Maleate:  neg Malonate:  neg L-Mandelate:  d Mesaconate:  neg Nonanoate:  neg Phenol:  + Phenylacetate:  neg Pimelate:  d Propionate:  neg Pyruvate:  + Quinate:  + Saccharate:  d Suberate:  d D-Tartrate:  d L-Tartrate:  d(neg) Valerate:  d
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  w α-Glucosidase:  d β-Glucosidase:  d AlkalineP:  d Lipase(C14):  d(neg)
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg Pigment:  yellow
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(10; disc)
    methicillin:  S(5; disc)
    kanamycin:  S(30; disc)
    neomycin:  S(30; disc)
    streptomycin:  S(10; disc)
    erythromycin:  S(15; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(30; disc)
    chloramphenicol:  S(30; disc)
    novobiocin:  S(30; disc)
    polymyxin_B:  S(300U)

    References

    SPECIFIC REFERENCES FOR HYDROGENOPHAGA PSEUDOFLAVA
  • Willems1989 - Hydrogenophaga, a New Genus of Hydrogen-Oxidizing Bacteria That Includes Hydrogenophaga flava comb. nov. (Formerly Pseudomonas flava), Hydrogenophaga palleronii (Formerly Pseudomonas palleronii), Hydrogenophaga pseudoflava (Formerly Pseudomonas pseudoflava and “Pseudomonas carboxydoflava”), and Hydrogenophaga taeniospiralis (Formerly Pseudomonas taeniospiralis)
  • Auling1978 - Pseudomonas pseudoflava, a New Species of Hydrogen-Oxidizing Bacteria: Its Differentiation from Pseudomonas flava and Other Yellow-Pigmented, Gram-Negative, Hydrogen-Oxidizing Species.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR HYDROGENOPHAGA PSEUDOFLAVA
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
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    • Added: February 09, 2022