Intestinimonas massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Intestinimonas massiliensis is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 60.7%. Intestinimonas massiliensis is a common gut coloniser. (Afouda2018; Durand2017a)



  • This organism has been recovered from human faeces (Afouda2018). Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • In 94% of Europeans (unseenbio.com).
    • GENERAL CHARACTERISTICS (Afouda2018); (Durand2017a);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin;
  • 🧂
  • Salt tolerance:
  • tolerates 0-0.5% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0-8.0; tolerates pH 8.5;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 28℃; grows at 37℃; doesn't grow at 45℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; D-fucose; galactose; ribose; sorbose; D-tagatose; xylose; L-xylose; amygdalin; aesculin; glycogen; inulin; starch; gentiobiose; D-lyxose; melezitose; melibiose; raffinose; trehalose; xylitol; 5-ketogluconate;
  • Active enzymes:
  • acid phosphatase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Afouda2018);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Afouda2018);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; imipenem; penicillin G;
  • Quinolines:
  • ofloxacin;
  • Vancomycins:
  • vancomycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Eubacteriales incertae sedi Genus:  Intestinimonas Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human faeces (Afouda2018)
    DNA G+C(%):  60.7
    Opt. T:  37℃
    Lower T(℃):  28(neg)
    Mid T(℃):  37(+)
    High T(℃):  45(neg)
    NaCl 0.5-2%:  0-0.5(+)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  8.5(+)
    		Gelatin:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  + L-Arabinose:  neg Fructose:  w Fucose:  neg D-Fucose:  + Galactose:  + Glucose:  neg Mannose:  neg D-Lyxose:  + Rhamnose:  neg Ribose:  + Sorbose:  + D-Tagatose:  + Xylose:  + L-Xylose:  + Cellubiose:  neg Gentiobiose:  + Lactose:  neg Maltose:  neg Melezitose:  + Melibiose:  + Sucrose:  neg Trehalose:  + Turanose:  neg Amygdalin:  + Aesculin:  + Glycogen:  + Inulin:  + Starch:  + Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  + Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  + Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Glucose:  neg Mannose:  neg Maltose:  neg N_Acetyl_glucosamine:  neg Gluconate:  neg Mannitol:  neg Adipate:  neg Caprate:  neg Citrate:  neg Malate:  neg Phenylacetate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg Chymotrypsin:  neg Trypsin:  neg LeuAA:  neg ValAA:  neg AlkalineP:  neg AcidP:  + Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  S(0.125)
    penicillin_G:  S(0.19)
    imipenem:  S(0.25)
    ofloxacin:  R(>32)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    vancomycin:  S(0.5)

    References

    SPECIFIC REFERENCES FOR INTESTINIMONAS MASSILIENSIS
  • Afouda2018 - Non-contiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2T, the second Intestinimonas species cultured from the human gut.
  • Durand2017a - 'Intestinimonas massiliensis' sp. nov, a new bacterium isolated from human gut.
  • Chen2020a - Featured Gut Microbiomes Associated With the Progression of Chronic Hepatitis B Disease
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR INTESTINIMONAS MASSILIENSIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • ...............................
    • GENERAL REFERENCES FOR INTESTINIMONAS MASSILIENSIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: July 12, 2021