Klebsiella pneumoniae subsp. ozaenae

(aka Klebsiella ozaenae)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Klebsiella pneumoniae subsp. ozaenae, (aka Klebsiella ozaenae), is a Gram-negative, non-spore-forming, facultatively anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. Klebsiella pneumoniae subsp. ozaenae is probably a rare gut coloniser. (Grimont2005aBergey)



  • This organism has been recovered from clinical sources (blood, nasopharynx- CCUG) and has been detected in human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. Could be a possible pathogen in humans, but unknown at this stage. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Grimont2005aBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • urea;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 5℃; grows at 41℃;
  • H+
  • Acid from carbohydrates usually produced:
  • glucose;
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; adonitol; d_arabitol; cellubiose; fructose; fucose; galactose; gluconate; glucosamine; gentiobiose; glucose; glycerol; inositol; lactose; maltose; maltotriose; mannitol; mannose; melibiose; methyl-α-glucoside; ribose; raffinose; trehalose; xylose; alanine; D-alanine; aspartate; proline; serine; citrate; fumarate; DL-glycerate; 2-ketogluconate; DL-lactate; malate; L-malate;
  • ±
  • Strain-dependent substrate utilisation:
  • rhamnose; sorbitol; sorbose; sucrose; mucate; phenylacetate; quinate; saccharate;
  • ±
  • Strain-dependent active enzymes:
  • lycine decarboxylase; urease;
    • SPECIAL FEATURES (Grimont2005aBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • formate; lactate; ethanol;
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✓
  • ONPG test:
  • active
  • KCN growth:
  • growth observed
    • RESPONSE TO ANTIBIOTICS (Citron1997);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; imipenem; piperacillin-tazobactam;
  • Cephalosporins:
  • cefotetan; cefoxitin;
  • Quinolines:
  • ciprofloxacin; levofloxacin; ofloxacin; trovafloxacin;
  • Aminoglycosides:
  • gentamicin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Enterobacterales Family:  Enterobacteriaceae Genus:  Klebsiella Alt. name:  Klebsiella ozaenae Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  clinical sources (blood, nasopharynx- CCUG) and has been detected in human faeces
    Low T(℃):  5(neg)
    High T(℃):  41(+)
    		Aesculin:  + Urea:  d DNA:  neg Pectin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  + Fucose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  d Ribose:  + Sorbose:  d D-Tagatose:  neg Xylose:  + Cellubiose:  + Gentiobiose:  + Lactose:  + Maltose:  + Maltotriose:  + Melezitose:  neg Melibiose:  + Raffinose:  + Sucrose:  d D-Turanose:  neg Trehalose:  + N_Acetyl_glucosamine:  + Adonitol:  + D-Arabitol:  + Dulcitol:  neg Erythritol:  neg Gluconate:  + Glucosamine:  + Glycerol:  + Inositol:  + Mannitol:  + Me-α-D-Glc:  + Sorbitol:  d Xylitol:  neg Ala:  + D-Ala:  + Asp:  + Betaine:  neg Pro:  + Ser:  + Trigonelline:  neg Tryptamine:  neg Tyr:  neg Benzoate:  neg Caprate:  neg Caprylate:  neg Citrate:  + Fumarate:  + Glutarate:  neg DL-Glycerate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Itaconate:  neg 2-Ketogluconate:  + DL-Lactate:  + Malate:  + L-Malate:  + Malonate:  neg Mucate:  d Phenylacetate:  d Phenylpropionate:  neg Propionate:  neg Quinate:  d Saccharate:  d D-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Urease:  d β-Glucuronidase:  neg LysDC:  d OrnDC:  neg Phe deaminase:  neg TrpAA:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  + Lactate:  + Ethanol:  + H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 4, MIC90: 8, RNG: (0.5–8)
    piper-taz:  S(MIC50): 0.5, MIC90: 0.5, RNG: (0.5–64)
    imipenem:  S(MIC50): 0.125, MIC90: 0.125, RNG: (0.125–0.25)
    cefotetan:  S(MIC50): 0.5, MIC90: 0.5, RNG: (0.5)
    cefoxitin:  S(MIC50): 2, MIC90: 2, RNG: (1–2)
    amikacin:  Var(MIC50): ≤0.25->32), MIC90: Var(≤0.25->32
    gentamicin:  S(MIC50): 0.5, MIC90: 0.5, RNG: (0.5)
    ciprofloxacin:  S(MIC50): 0.015, MIC90: 0.03, RNG: (0.015–0.03)
    levofloxacin:  S(MIC50): 0.015, MIC90: 0.015, RNG: (0.15–0.06)
    ofloxacin:  S(MIC50): 0.03, MIC90: 0.06, RNG: (0.015–0.125)
    trovafloxacin:  S(MIC50): 0.03, MIC90: 0.06, RNG: (0.015–0.06)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)

    References

    SPECIFIC REFERENCES FOR KLEBSIELLA PNEUMONIAE SUBSP. OZAENAE
  • Grimont2005aBergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Enterobacteriaceae, Genus XVI. Klebsiella
  • Citron1997 - Comparative in vitro activities of trovafloxacin (CP-99,219) against 221 aerobic and 217 anaerobic bacteria isolated from patients with intra-abdominal infections.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR KLEBSIELLA PNEUMONIAE SUBSP. OZAENAE
  • Finegold1974 - Effect of diet on human fecal flora: comparison of Japanese and American diets
  • Finegold1977 - Fecal microbial flora in Seventh Day Adventist populations and control subjects.
  • Zou2019 - 1,520 reference genomes from cultivated human gut bacteria enable functional microbiome analyses.
  • ...............................
    • Added: January 06, 2022