Kushneria sinocarnis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Kushneria sinocarnis is a Gram-negative, non-spore-forming, aerobic, non-motile, oval-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 59.1%. Kushneria sinocarnis is likely to be transient and not a long-term gut coloniser. (Zou2010)



  • This organism has been recovered from cured meat from China. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Unlikely gut coloniser because its optimum growth is with high salt.
    • GENERAL CHARACTERISTICS (Zou2010);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 0% salt; tolerates 20% salt; Grows optimally with 10% salt.
  • 🌡
  • Temperature tolerance:
  • grows at 4℃; grows at 42℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; fructose; glucose; mannose; rhamnose; sorbose; xylose; lactose; maltose; trehalose; adonitol; mannitol; sorbitol; salicin;
  • ⥅
  • Substrates assimilated or utilised:
  • L-arabinose; fructose; galactose; gluconate; glucose; glycerol; maltose; mannose; melibiose; ribose; sorbitol; sucrose; trehalose; xylose; glutamate; isoleucine; proline; serine; citrate; fumarate; lactate; pyruvate; succinate; glutamine;
  • Active enzymes:
  • catalase; β-galactosidase;
    • SPECIAL FEATURES (Zou2010);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✓
  • Nitrate:
  • reduced
  • ✗
  • Nitrite:
  • not reduced
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Zou2010);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; ampicillin;
  • Cephalosporins:
  • cefotaxime; cefoxitin;
  • Macrolides:
  • erythromycin;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • kanamycin; streptomycin; tobramycin;
  • Heterocycles:
  • nitrofurantoin;
  • chloramphenicol; trimethoprim-sulfamethoxazole;
  • Miscellaneous antibiotics:
  • polymyxin B;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Planococcaceae Genus:  Kushneria Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Oval-shaped
    Health:  Unknown
    Source:  cured meat from China
    DNA G+C(%):  59.1
    Opt. T:  37℃
    Low T(℃):  4(+)
    High T(℃):  42(+)
    NaCl 0.5-2%:  0(neg)
    NaCl >6%:  20(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Starch:  neg Casein:  neg DNA:  neg Tyrosine:  neg Tween:  20(+) 80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  + Fructose:  + Glucose:  + Mannose:  + Rhamnose:  + Ribose:  neg Sorbose:  + Xylose:  + Lactose:  + Maltose:  + Melezitose:  neg Sucrose:  neg Trehalose:  + Adonitol:  + Inositol:  neg Mannitol:  + Sorbitol:  + Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  + Fructose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  + Xylose:  + Cellubiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  + Raffinose:  neg Sucrose:  + Trehalose:  + N_Acetyl_glucosamine:  neg Adonitol:  neg Aesculin:  neg Ethanol:  neg Gluconate:  + Glycerol:  + Inositol:  neg Methanol:  neg Salicin:  neg Sorbitol:  + Starch:  neg Ala:  neg Arg:  neg Asp:  neg Cys:  neg Glu:  + His:  neg Ile:  + Leu:  neg Lys:  neg Met:  neg Orn:  neg Phe:  neg Pro:  + Ser:  + Thr:  neg Trp:  neg Val:  neg Acetate:  neg Benzoate:  neg Caprylate:  neg Citrate:  + Formate:  neg Fumarate:  + 3-Hydroxybutyrate:  neg Lactate:  + Malate:  neg Malonate:  neg Propionate:  neg Pyruvate:  + Succinate:  + D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg β-Galactosidase:  + ArgDH:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg DNAse:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  R(20; disc)
    ampicillin:  R(10U)
    cefotaxime:  R(30; disc)
    cefoxitin:  R(30; disc)
    kanamycin:  R(30; disc)
    streptomycin:  R(10; disc)
    tobramycin:  R(10; disc)
    erythromycin:  S(15; disc)
    nalidixic-acid:  R(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    chloramphenicol:  R(30; disc)
    nitrofurantoin:  S(300; disc)
    SXT:  R(5; disc)
    polymyxin_B:  R(25; disc)

    References

    SPECIFIC REFERENCES FOR KUSHNERIA SINOCARNIS
  • Zou2010 - Kushneria sinocarnis sp. nov., a moderately halophilic bacterium isolated from a Chinese traditional cured meat.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR KUSHNERIA SINOCARNIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • ...............................
    • Added: January 14, 2022