Lancefieldella parvula

(aka Atopobium parvulum)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Lancefieldella parvula, (aka Atopobium parvulum), is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, coccus bacterium. It has been detected in at least 14 gut microbiome compilation studies or metastudies. The DNA G+C content is 35-41%. Lancefieldella parvula is probably a common, although minor, coloniser of the gut. (Olsen1991)



  • This organism has been recovered from dental disease, clinical sources (wound, blood, abscess - CCUG) and human faeces. Can cause opportunistic infections, particularly in immunocompromised people. A possible gut commensal.
    • QUIRKS
  • Also known as Anaerostipes sp 3 2 56FAA and Peptostreptococcus parvulus
    • GENERAL CHARACTERISTICS (Olsen1991);
    Character Response
  • ±
  • Strain-dependent hydrolysis or digestion:
  • aesculin; milk;
  • 🧂
  • Salt tolerance:
  • tolerates 6.5% salt;
  • 🌡
  • Temperature tolerance:
  • strain-variable at 45(d);
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; inulin; lactose; maltose; sucrose; trehalose;
  • ±
  • Strain-dependent acid from carbs:
  • ribose; xylose; aesculin; salicin;
    • SPECIAL FEATURES (Olsen1991);
    Character Response
  • ✓
  • Metabolites produced:
  • formate (minor); acetate; lactate (major); succinate (trace);
  • ✗
  • Metabolites not produced:
  • ammonia; Hâ‚‚; indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Olsen1991); (Goldstein2013); (Tyrrell2012);
    Class Active Resistant
  • Penicillins:
  • ampicillin; imipenem; penicillin G; piperacillin-tazobactam;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • moxifloxacin;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; linezolid;

  • Finegold, S. M., Howard, R. A., & Vera, L. S. (1974). Effect of diet on human intestinal fecal flora: comparison of Japanese and American diets. Am. J. Clin. Nutr, 27, 1456–1469.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Coriobacteriia Order:  Coriobacteriales Family:  Atopobiaceae Genus:  Lancefieldella Alt. name:  Atopobium parvulum Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus
    Health:  Unknown
    Source:  dental disease, clinical sources (wound, blood, abscess - CCUG) and human faeces
    DNA G+C(%):  35-41
    High T(℃):  45(d)
    NaCl >6%:  6.5(+)
    		Aesculin:  d Gelatin:  neg Starch:  neg Arginine:  neg DNA:  neg Hippurate:  neg Milk:  curdle Meat:  neg Tween:  80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  d(neg) Xylose:  d(neg) Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  neg Sucrose:  + Trehalose:  + Amygdalin:  neg Aesculin:  d(neg) Glycogen:  neg Inulin:  d(+) Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Salicin:  d
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  minor(+) Acetate:  + Lactate:  Major(+) Succinate:  trace(+) Ammonia:  neg H2:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.03–1)
    penicillin_G:  S(2U)
    piper-taz:  S(MIC50): 0.25, MIC90: 1, RNG: (0.015–1)
    imipenem:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.015–0.5)
    erythromycin:  S(3)
    linezolid:  S(MIC50): 1, MIC90: 4, RNG: (0.25–8)
    moxifloxacin:  S(MIC50): 0.5, MIC90: 1, RNG: (0.06–8)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(6)
    vancomycin:  S(MIC50): 1, MIC90: 2, RNG: (0.25–4.0)
    chloramphenicol:  S(12)
    metronidazole:  S(MIC50): 0.5, MIC90: 2, RNG: (0.125–4)
    clindamycin:  S(1.6, vr)
    daptomycin:  Var(MIC50): 0.5, MIC90: 16, RNG: (0.06–32)

    References

    SPECIFIC REFERENCES FOR LANCEFIELDELLA PARVULA
  • Olsen1991 - Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. from the Human Gingival Crevice and Emended Descriptions of Lactobacillus minutus and Streptococcus parvulus.
  • Jie2017 - The gut microbiome in atherosclerotic cardiovascular disease
  • Yachida2019 - Metagenomic and metabolomic analyses reveal distinct stage-specific phenotypes of the gut microbiota in colorectal cancer
  • Goldstein2013 - In vitro activity of Biapenem plus RPX7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria.
  • Tyrrell2012 - In vitro activity of TD-1792, a multivalent glycopeptide-cephalosporin antibiotic, against 377 strains of anaerobic bacteria and 34 strains of Corynebacterium species.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR LANCEFIELDELLA PARVULA
  • Benno1986 - Comparison of the fecal microflora in rural Japanese and urban Canadians.
  • Benno1989 - Comparison of fecal microflora of elderly persons in rural and urban areas of Japan.
  • Bik2006 - Molecular analysis of the bacterial microbiota in the human stomach.
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Finegold1974 - Effect of diet on human fecal flora: comparison of Japanese and American diets
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • Jie2017 - The gut microbiome in atherosclerotic cardiovascular disease
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • Added: April 08, 2021