Lancefieldella rimae

(aka Lactobacillus rimae)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Lancefieldella rimae, (aka Lactobacillus rimae), is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 5 gut microbiome compilation studies or metastudies. The DNA G+C content is 45-46%. Lancefieldella rimae is probably a common, although minor, coloniser of the gut. (Olsen1991)



  • This organism has been recovered from dental disease, clinical sources (blood, abscess - CCUG) and human faeces. It is an opportunistic pathogen. A possible gut commensal.
    • QUIRKS
  • In 11% of Europeans (unseenbio.com). Minor coloniser.
    • GENERAL CHARACTERISTICS (Olsen1991);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; arginine;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • starch;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 6.5% salt;
  • 🌡
  • Temperature tolerance:
  • strain-variable at 45(d);
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; cellubiose; maltose; sucrose; trehalose;
  • ±
  • Strain-dependent acid from carbs:
  • arabinose; ribose; inulin; salicin;
    • SPECIAL FEATURES (Olsen1991);
    Character Response
  • ✓
  • Metabolites produced:
  • formate (minor); acetate; lactate (major); succinate (trace); ammonia;
  • ✗
  • Metabolites not produced:
  • Hâ‚‚; indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Goldstein2013); (Tyrrell2012); (Olsen1991);
    Class Active Resistant
  • Penicillins:
  • ampicillin; imipenem; penicillin G; piperacillin-tazobactam;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • moxifloxacin;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; linezolid;
  • NOTES

    The type strain for Atopobium rimae was isolated from a human gingival crevice.

  • While ATCC lists Atopobium rimae as BSL1 there are literature reports of isolation of Atopobium rimae from patients. Therefore, this bacterium shoudl be treated as a potential pathogen.

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Coriobacteriia Order:  Coriobacteriales Family:  Atopobiaceae Genus:  Lancefieldella Alt. name:  Lactobacillus rimae Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  dental disease, clinical sources (blood, abscess - CCUG) and human faeces
    DNA G+C(%):  45-46
    High T(℃):  45(d)
    NaCl >6%:  6.5(neg)
    		Aesculin:  d(+) Gelatin:  neg Starch:  d(neg) Arginine:  d(+) DNA:  neg Hippurate:  neg Milk:  neg Meat:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  d Fructose:  + Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  d(neg) Xylose:  neg Cellubiose:  d(+) Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Sucrose:  + Trehalose:  + Amygdalin:  neg Aesculin:  neg Inulin:  d(neg) Starch:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Salicin:  d
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  minor(+) Acetate:  + Lactate:  Major(+) Succinate:  trace(+) Ammonia:  + H2:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.03–1)
    penicillin_G:  S(2U)
    piper-taz:  S(MIC50): 0.25, MIC90: 1, RNG: (0.015–1)
    imipenem:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.015–0.5)
    erythromycin:  S(3)
    linezolid:  S(MIC50): 1, MIC90: 4, RNG: (0.25–8)
    moxifloxacin:  S(MIC50): 0.5, MIC90: 1, RNG: (0.06–8)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(6)
    vancomycin:  S(MIC50): 1, MIC90: 2, RNG: (0.25–32)
    chloramphenicol:  S(12)
    metronidazole:  S(MIC50): 0.5, MIC90: 2, RNG: (0.125–4)
    clindamycin:  S(1.6)
    daptomycin:  Var(MIC50): 0.5, MIC90: 16, RNG: (0.06–32)

    References

    SPECIFIC REFERENCES FOR LANCEFIELDELLA RIMAE
  • Goldstein2013 - In vitro activity of Biapenem plus RPX7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria.
  • Tyrrell2012 - In vitro activity of TD-1792, a multivalent glycopeptide-cephalosporin antibiotic, against 377 strains of anaerobic bacteria and 34 strains of Corynebacterium species.
  • Olsen1991 - Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. from the Human Gingival Crevice and Emended Descriptions of Lactobacillus minutus and Streptococcus parvulus.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR LANCEFIELDELLA RIMAE
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • Added: April 08, 2021