Leclercia adecarboxylata

(aka Escherichia adecarboxylata)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Leclercia adecarboxylata, (aka Escherichia adecarboxylata), is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 52.4-54.8%. Leclercia adecarboxylata is probably a rare gut coloniser. (Tamura1986; O'Hara2005aBergey)



  • This organism has been recovered from clinical sources (throat, blood, cerebrospinal fluid - CCUG) and human faeces (CCUG; including ulcerative colitis patients - Alkhalil2017). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. Could be a possible pathogen in humans, but unknown at this stage. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Tamura1986); (O'Hara2005aBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🌡
  • Temperature tolerance:
  • strain-variable at 4(d); grows at 37℃; doesn't grow at 42℃;
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; rhamnose; ribose; xylose; cellubiose; gentiobiose; lactose; maltose; melibiose; trehalose; adonitol; D-arabitol; dulcitol; mannitol; arbutin; gluconate; 2-ketogluconate; salicin;
  • ±
  • Strain-dependent acid from carbs:
  • mucate; raffinose; sucrose;
  • ⥅
  • Substrates assimilated or utilised:
  • adonitol; L-arabinose; d_arabitol; arbutin; cellubiose; dulcitol; aesculin; fructose; galactose; glucosamine; gentiobiose; glucose; lactose; maltose; mannitol; mannose; melibiose; rhamnose; ribose; salicin; trehalose; xylose; alanine; D-alanine; aspartate; glutamate; histidine; proline; serine; 2-ketogluconate; 2-ketoglutarate; DL-lactate; L-malate; mucate; pyruvate; succinate; glutamine; D-malate;
  • ±
  • Strain-dependent substrate utilisation:
  • amygdalin; D-lyxose; raffinose; sucrose; fumarate; DL-glycerate; phenylacetate; L-tartrate;
  • Active enzymes:
  • β-galactosidase; xylosidase;
    • SPECIAL FEATURES (Tamura1986); (O'Hara2005aBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • indole;
  • ✗
  • Metabolites not produced:
  • H₂S;
  • ✓
  • Methyl red test:
  • active
  • ✗
  • VP test:
  • not active
  • ✓
  • ONPG test:
  • active
  • KCN growth:
  • growth observed
  • ✓
  • NO3➔NO2:
  • reduced
  • Pigments:
  • yellow (atypical)
    • RESPONSE TO ANTIBIOTICS (Tamura1986); (O'Hara2005aBergey);
    Class Active Resistant
  • Penicillins:
  • ampicillin; carbenicillin;
  • penicillin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; kanamycin; streptomycin;
  • Heterocycles:
  • chloramphenicol; sulfadiazine;
  • Miscellaneous antibiotics:
  • colistin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Enterobacterales Family:  Enterobacteriaceae Genus:  Leclercia Alt. name:  Escherichia adecarboxylata Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  yellow (atypical)
    Health:  Unknown
    Source:  clinical sources (throat, blood, cerebrospinal fluid - CCUG) and human faeces (CCUG; including ulcerative colitis patients - Alkhalil2017)
    DNA G+C(%):  52.4-54.8
    Low T(℃):  4(d)
    Mid T(℃):  37(+)
    High T(℃):  42(neg)
    		Aesculin:  + Gelatin:  neg DNA:  neg Pectin:  neg Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  neg Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  + Gentiobiose:  + Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  + Sucrose:  d Trehalose:  + Turanose:  neg Amygdalin:  neg Adonitol:  + D-Arabitol:  + L-Arabitol:  neg Dulcitol:  + Erythritol:  neg Inositol:  neg Mannitol:  + Sorbitol:  neg Xylitol:  neg Arbutin:  + Gluconate:  + 2-Ketogluconate:  + 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  + Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + D-Lyxose:  d Mannose:  + Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  + Cellubiose:  + Gentiobiose:  d(+) Lactose:  d(+) Maltose:  + Melezitose:  neg Melibiose:  + Raffinose:  d Sucrose:  d D-Turanose:  neg Trehalose:  + Adonitol:  d(+) Amygdalin:  d D-Arabitol:  d(+) L-Arabitol:  neg Arbutin:  + Dulcitol:  d(+) Erythritol:  neg Aesculin:  + Ethanolamine:  neg Glucosamine:  + Inositol:  neg Mannitol:  + Me-α-D-Glc:  neg Salicin:  + Xylitol:  neg Ala:  + D-Ala:  + β-Ala:  neg 4-Aminobutyrate:  neg 5-Aminovalerate:  neg Arg:  neg Asp:  + L-Citrulline:  neg Cys:  neg Glu:  + Gly:  neg Histamine:  neg His:  + Ile:  neg DL-Kynurenine:  neg Leu:  neg Lys:  neg Met:  neg Norleucine:  neg Orn:  neg Phe:  neg Pro:  + Sarcosine:  neg Ser:  + Thr:  neg Trigonelline:  neg Tryptamine:  neg Trp:  neg Tyr:  neg Val:  neg Acetate:  neg Aconitate:  neg Adipate:  neg Azelate:  neg Benzoate:  neg Butyrate:  neg Caprate:  neg Caproate:  neg Caprylate:  neg Citraconate:  neg Citrate:  neg Fumarate:  d Glutarate:  neg DL-Glycerate:  d Glycolate:  neg Heptanoate:  neg 3-Hydroxybutyrate:  neg 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Isobutyrate:  neg Itaconate:  neg 2-Ketogluconate:  + 5-Ketogluconate:  neg DL-Lactate:  + Levulinate:  neg L-Malate:  + Maleate:  neg Malonate:  vr L-Mandelate:  neg Mesaconate:  neg Mucate:  d(+) Nonanoate:  neg Phenylacetate:  d Pimelate:  neg Propionate:  neg Pyruvate:  + Sebacate:  neg Suberate:  neg Succinate:  d(+) D-Tartrate:  neg L-Tartrate:  d(neg) Valerate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Urease:  neg β-Galactosidase:  + β-Glucuronidase:  neg Xylosidase:  + ArgDH:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  + Pigment:  yellow (atypical)
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  Sens
    carbenicil:  Sens
    penicillin:  Res
    gentamicin:  Sens
    kanamycin:  Sens
    streptomycin:  Sens
    nalidixic-acid:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  Sens
    chloramphenicol:  Sens
    sulfadiazine:  Sens
    colistin:  Sens

    References

    SPECIFIC REFERENCES FOR LECLERCIA ADECARBOXYLATA
  • Tamura1986 - Leclercia adecarboxylata Gen. Nov., Comb. Nov., formerly known as Escherichia adecarboxylata.
  • O'Hara2005aBergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Enterobacteriaceae, Genus XVIII. Leclercia
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR LECLERCIA ADECARBOXYLATA
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • ...............................
    • GENERAL REFERENCES FOR LECLERCIA ADECARBOXYLATA
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
  • Alkhalil2017 - Bacterial involvements in ulcerative colitis: molecular and microbiological studies
    • Added: July 12, 2021