Lentilactobacillus buchneri subsp. buchneri

(aka Lactobacillus buchneri)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Lentilactobacillus buchneri subsp. buchneri, (aka Lactobacillus buchneri), is a Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. The DNA G+C content is 44–46%. Lentilactobacillus buchneri subsp. buchneri is probably a common, although minor, coloniser of the gut. (Pederson1938; Hammes2011Bergey; Rossi2016)



  • This organism has been recovered from silage, dairy products, the oral cavity and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal. Robust growth can have positive consequences for gut health.
    • GENERAL CHARACTERISTICS (Pederson1938); (Hammes2011Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; arginine;
  • 🌡
  • Temperature tolerance:
  • grows at 10℃; grows at 45℃; Grows optimally at 30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; fructose; galactose; glucose; ribose; xylose; lactose; maltose; melezitose; melibiose; sucrose;
  • ⥅
  • Substrates assimilated or utilised:
  • melibiose;
  • Active enzymes:
  • acid phosphatase; arginine dihydrolase; cystine arylamidase; α-galactosidase; β-galactosidase; α-glucosidase; leucine aminopeptidase; Val arylamidase;
    • SPECIAL FEATURES (Pederson1938); (Hammes2011Bergey);
    Character Response
  • ✓
  • Metabolites produced:
  • lactate (major); ammonia;
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • VP test:
  • active
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • streptomycin;
  • Heterocycles:
  • chloramphenicol;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;

  • Mitsuoka, T, K Hayakawa & N Kimura (1975). The fecal flora of man. III. Communication: The composition of Lactobacillus flora of different age groups. Zentralbl. Bakteriol. 232: 499-501.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Lactobacillales Family:  Lactobacillaceae Genus:  Lentilactobacillus Alt. name:  Lactobacillus buchneri Gram stain:  + O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:   Positive
    Source:  silage, dairy products, the oral cavity and human faeces
    DNA G+C(%):  44–46
    Opt. T:  30℃
    Low T(℃):  10(+)
    High T(℃):  45(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Casein:  neg Arginine:  + Hippurate:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  vr D-Arabinose:  neg L-Arabinose:  + Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  neg D-Lyxose:  neg Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  + Maltose:  + Melezitose:  + Melibiose:  + Sucrose:  d(+) Trehalose:  neg Turanose:  vr Amygdalin:  neg Dextrin:  neg Glycogen:  neg Inulin:  vr Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  vr 2-Ketogluconate:  neg 5-Ketogluconate:  vr Me-α-D-Glc:  vr Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg β-Mannosidase:  neg ArgDH:  + LeuAP:  + AlaPheProAA:  neg CystineAA:  + PyrrolidAA:  neg ValAA:  + AlkalineP:  neg AcidP:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Lactate:  Major(+) H2S:  neg Ammonia:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  RNG: (0.25-1)
    ampicillin:  RNG: (0.5-2)
    penicillin_G:  RNG: (1-8)
    gentamicin:  RNG: (1-2)
    kanamycin:  RNG: (4-64)
    neomycin:  RNG: (1-8)
    streptomycin:  R(8-32)
    erythromycin:  SensRNG: (≤0.125-0.25)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  R(32-128)
    vancomycin:  R(≥256-)
    chloramphenicol:  SensRNG: (4-)
    trimethoprim:  RNG: (0.5-128)
    clindamycin:  SensRNG: (≤0.125-1)
    lincomycin:  RNG: (2-32)

    References

    SPECIFIC REFERENCES FOR LENTILACTOBACILLUS BUCHNERI SUBSP. BUCHNERI
  • Anzengruber2014 - Biochemical characterization of the major N-acetylmuramidase from Lactobacillus buchneri.
  • Pederson1938 - The Gas-producing Species of the Genus Lactobacillus.
  • Tanizawa2020 - Lactobacillus buchneri subsp. silagei subsp. nov., isolated from rice grain silage.
  • Hammes2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Lactobacillaceae, Genus I. Lactobacillus
  • Liu2020a - Proposal to reclassify Lactobacillus zhaodongensis, Lactobacillus zeae, Lactobacillus argentoratensis and Lactobacillus buchneri subsp. silagei as Lacticaseibacillus zhaodongensis comb. nov., Lacticaseibacillus zeae comb. nov., Lactiplantibacillus argentoratensis comb. nov. and Lentilactobacillus buchneri subsp. silagei comb. nov., respectively and Apilactobacillus kosoi as a later heterotypic synonym of Apilactobacillus micheneri
  • Rossi2016 - Mining metagenomic whole genome sequences revealed subdominant but constant Lactobacillus population in the human gut microbiota.
  • Gryp2020 - Isolation and Quantification of Uremic Toxin Precursor-Generating Gut Bacteria in Chronic Kidney Disease Patients
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR LENTILACTOBACILLUS BUCHNERI SUBSP. BUCHNERI
  • Benno1986 - Comparison of the fecal microflora in rural Japanese and urban Canadians.
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Tyakht2013 - Human gut microbiota community structures in urban and rural populations in Russia.
  • Woodmansey2004 - Comparison of compositions and metabolic activities of fecal microbiotas in young adults and in antibiotic-treated and non-antibiotic-treated elderly subjects.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR LENTILACTOBACILLUS BUCHNERI SUBSP. BUCHNERI
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021